2020
DOI: 10.1042/bcj20190050
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Abstract: Physiological membrane vesicles are built to separate reaction spaces in a stable manner, even when they accidentally collide or are kept in apposition by spatial constraints in the cell. This requires a natural resistance to fusion and mixing of their content, which originates from substantial energetic barriers to membrane fusion [1]. To facilitate intracellular membrane fusion reactions in a controlled manner, proteinaceous fusion machineries have evolved. An important open question is whether protein fusio… Show more

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Cited by 24 publications
(23 citation statements)
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“…Unlike LotA, LotB has a clear host target resulting in modulation of the early secretory pathway [112]. Membrane fusion in eukaryotes requires the pairing of proteins called soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) [113]. The host vesicular SNARE, Sec22B is recruited to the LCV and binds to the t-SNARE, Stx3 to help establish the early LCV membrane [114][115][116].…”
Section: Deubiquitinasesmentioning
confidence: 99%
“…Unlike LotA, LotB has a clear host target resulting in modulation of the early secretory pathway [112]. Membrane fusion in eukaryotes requires the pairing of proteins called soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) [113]. The host vesicular SNARE, Sec22B is recruited to the LCV and binds to the t-SNARE, Stx3 to help establish the early LCV membrane [114][115][116].…”
Section: Deubiquitinasesmentioning
confidence: 99%
“…Even the presence of three Yeast SNARE complexes at the fusion site still yields a residual barrier of about 70 k B T against expansion of the stalk (D'Agostino et al 2017). Fusion within the low curvature regime may thus rely on the local generation of high membrane curvature (Risselada and Mayer 2020). Interestingly, the additional presence of a single voluminous SNARE-associated tether complex can already reduce the free energy barrier against stalk widening to about 35 k B T by imposing steric repulsions on the site of hemifusion (Fig.…”
Section: Expansion Of the Stalk In Snare-mediated Fusionmentioning
confidence: 99%
“…In the last decades, much understanding has been gained about the intermediate steps of the biological fusion reaction due to extensive theoretical and experimental research. It has now become widely accepted that the fusion reaction must proceed through a hemifusion state before the fusion reaction can progress into the formation of a fusion pore Kozlov 2005, 2008;Risselada and Mayer 2020).…”
Section: Introductionmentioning
confidence: 99%
“…GEFs catalyze Rabs' GTP–GDP exchange, thus activating Rabs (Lamber et al, 2019). A GTP‐bound, activated Rab would, in turn, engage its effectors, which may include a myriad of membrane trafficking‐associated molecules such as coat proteins (Angers & Merz, 2011), cytoskeletal motor proteins (Delevoye & Goud, 2015), as well as SNAREs and membrane tethering factors (Risselada & Mayer, 2020). Rabs have generally low intrinsic GTPase activities, and GTP hydrolysis leading to their inactivation is typically accelerated through the engagement of GAPs (Lamber et al, 2019).…”
Section: Introductionmentioning
confidence: 99%