A fluorometric and colorimetric dual-signal nanoplatform for ultrasensitive visual monitoring of the activity of alkaline phosphatase

Abstract

Considering the limited sensitivity and accuracy of single-signal assay strategies, the multi-signal assay strategy has sparked significant excitement in recent years.

High-quality full-color carbon quantum dots synthesized under an unprecedentedly mild condition

“…Thus, DETA together with the other three phenylenediamines were used for the synthesis of CQDs in the followed experiment. It is notable that the use of DETA and another precursor containing only sp 2 carbon ( p -aminophenol) in preparing bright blue CQDs under mild conditions also succeeded before ( An et al., 2021 ).…”

“…The strong vibration bands of C=N were observed at 1510 cm −1 ( Chen et al., 2021 ; Weetman et al., 2019 ). Bands at 3435, 1651, 1380, and 1100 cm −1 were ascribed to the stretching vibration of N-H, C=O, C-N, and C-O ( An et al., 2021 ; Chen et al., 2021 ), respectively, which also demonstrated that the dehydrate condensation occurred between the carboxyl and amine groups. X-ray photoelectron spectroscopy (XPS) further verified the presence of carboxyl and amine in the CQDs ( Figure S17 ) ( Weetman et al., 2019 ).…”

“…Even the electrochemical methods are much greener and safer than their counterparts ( Wang et al., 2014 ), and extra separation steps for obtaining optically homogeneous CQDs are required ( Bao et al., 2011 ). Besides, the recently reported mild “bottom-up” methods by using phenols and ethylenediamine/diethylenetriamine as the precursors only produced blue and green CQDs ( An et al., 2021 ; Liu et al., 2020 ; Zhang et al., 2018 ).…”

A label-free dual-modal aptasensor for colorimetric and fluorescent detection of sulfadiazine

“…26 In contrast, this sensor based on dual-modal response can provide more reliable results on account of two independent readout signals. 27 In recent years, to rapid and accurate detect the analytes dual-modal aptasensors have been fabricated by combining the visualization of colorimetric signals with the high sensitivity of fluorescence signals. As reported by Wang et al , a colorimetric-fluorescent dual-modal aptasensor based on aptamer-modified AuNPs and fluorescein (FAM)-labeled complementary strands was developed to detect the parvalbumin.…”

A smartphone-based device for simultaneous measurement of ratiometric fluorescence and absorbance demonstrated by the determination of hypochlorous acid

“…1–4 In the widely used fluorometric and colorimetric dual-mode, the fluorescence intensity and absorbance signals are measured, respectively, using a spectrofluorophotometer and a spectrophotometer. 5–11 It is beneficial to develop a simple analytical device in dual-mode to measure simultaneously the fluorescence intensity and absorbance, which is important for field analysis.…”