Development of potent inhibitors for strigolactone receptor DWARF 14

Yoshimura, Masahiko; Kim, Sojung F.; Takise, Ryosuke; Kusano, Shuhei; Nakamura, Sakuya; Izumi, Masanori; Yagi, Akiko; Itami, Kenichiro; Hagihara, Shinya

doi:10.1039/d0cc01989e

Cited by 5 publications

(4 citation statements)

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“…Synthetic inhibitors KK094 (Nakamura et al, 2019 ), TFA (Hamiaux et al, 2018 ), and DL1b (Yoshimura et al, 2020 ) of D14 SL receptors have been described in Arabidopsis and petunia. Their discovery was based especially on their aptitude to inhibit the hydrolysis of (±)-YLG in competition assays with SL receptors.…”

Section: Discussionmentioning

confidence: 99%

“…The development of a variant of YLG (YLGW) allowed for the visualization of SL receptor activity in germinating Striga hermonthica seeds (Tsuchiya et al, 2018 ). The YLG is commercially available and has been used thereafter to identify SL receptor antagonists as tolfenamic acid (Hamiaux et al, 2018 ), KK094, and DL1b (Nakamura et al, 2019 ; Yoshimura et al, 2020 ) toward DAD2 and AtD14 proteins, respectively, highlighting the usefulness of this probe. Very recently, a novel resorufin-based SL profluorescent probe, Xilatone Red (XLR) based on a resorufin moiety has been developed (Wang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

et al. 2022

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Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

et al. 2022

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“…67) In addition, by performing structural optimization of DL1, DL1b was found to increase the branching of Arabidopsis and rice at a lower concentration. 68) Al-Babilli et al found Triton X-100 acts as a selective inhibitor of ShHTL/ShKAI2d. 69) They had used Triton X-100 as a detergent during ShHTL7 purification.…”

Section: Receptor Inhibition In Parasitic Plantsmentioning

confidence: 98%

“… 67 ) In addition, by performing structural optimization of DL1, DL1b was found to increase the branching of Arabidopsis and rice at a lower concentration. 68 )…”

Section: Receptor Inhibition In Parasitic Plantsmentioning

confidence: 99%

Emerging technologies for the chemical control of root parasitic weeds

et al. 2022

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Parasitic plants in the Orobanchaceae family include devastating weed species, such as Striga, Orobanche, and Phelipanche, which parasitize major crops, drastically reduces crop yields and cause economic losses of over a billion US dollars worldwide. Advances in basic research on molecular and cellular processes responsible for parasitic relationships has now achieved steady progress through advances in genome analysis, biochemical analysis and structural biology. On the basis of these advances it is now possible to develop chemicals that control parasitism and reduce agricultural damage. In this review we summarized the recent development of chemicals that can control each step of parasitism from strigolactone biosynthesis in host plants to haustorium formation.

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Design, Synthesis and Biological Evaluation of Novel 1H-1,2,4-Triazole Derivatives as Strigolactone Biosynthesis Inhibitors

Du,

Yan,

et al. 2023

J Plant Growth Regul

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Development of potent inhibitors for strigolactone receptor DWARF 14

Abstract: Strigolactones (SLs) are plant hormones that suppress shoot branching through perception by their receptor protein DWARF 14 (D14). Based on the binding model of DL1, a small-molecule D14 inhibitor, more potent compounds were developed.

Cited by 5 publications

References 20 publications

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

Emerging technologies for the chemical control of root parasitic weeds

Design, Synthesis and Biological Evaluation of Novel 1H-1,2,4-Triazole Derivatives as Strigolactone Biosynthesis Inhibitors

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