Protein delivery into cells using inorganic nanoparticle–protein supramolecular assemblies

Scaletti, Federica; Hardie, Joseph; Lee, Yi-Wei; Luther, David C.; Ray, Moumita; Rotello, Vincent M.

doi:10.1039/c8cs00008e

Cited by 160 publications

(123 citation statements)

References 53 publications

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“…135,136 AuNPs can be an ideal carrier beneting from their chemical functionality and biological inertness. 137,138 Researchers have developed cationic arginine-modied gold nanoparticles to form nanoassemblies for co-delivery of Cas9 protein and sgRNA into cells. 139 This construct provides high efficiency in vitro cell gene editing.…”

Section: Functionalized-aunps For Cancer Therapymentioning

confidence: 99%

Surface chemistry of gold nanoparticles for health-related applications

2020

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Section: Functionalized-aunps For Cancer Therapymentioning

confidence: 99%

Surface chemistry of gold nanoparticles for health-related applications

2020

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“…Therefore, direct penetration and cytosol delivery strategies that allow avoidance of endolysosomes are attracting increasing interest. [ 41 ]…”

Section: Cellular Uptake Mechanism For Nanoparticlesmentioning

confidence: 99%

Nanocarrier‐Mediated Cytosolic Delivery of Biopharmaceuticals

Xing

Wang

et al. 2020

Adv Funct Materials

103

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Biopharmaceuticals have emerged to play a vital role in disease treatment and have shown promise in the rapidly expanding pharmaceutical market due to their high specificity and potency. However, the delivery of these biologics is hindered by various physiological barriers, owing primarily to the poor cell membrane permeability, low stability, and increased size of biologic agents. Since many biological drugs are intended to function by interacting with intracellular targets, their delivery to intracellular targets is of high relevance. In this review, the authors summarize and discuss the use of nanocarriers for intracellular delivery of biopharmaceuticals via endosomal escape and, especially, the routes of direct cytosolic delivery by means including the caveolae‐mediated pathway, contact release, intermembrane transfer, membrane fusion, direct translocation, and membrane disruption. Strategies with high potential for translation are highlighted. Finally, the authors conclude with the clinical translation of promising carriers and future perspectives.

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“…[18] Either by noncovalent interaction or covalent attachment, proteins could be encapsulated or stabilized by these NPs.…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

“…[15] A wide array of nanocarrier systems including lipid, polymers, protein complex, inorganic materials have been shown to be promising strategies. [16][17][18][19] In these strategies, different methods are applied to load the delivery system with the targeted proteins, such as genetic modification, physical adsorption, or chemical conjugation. In addition, these protein delivery systems based on NPs could be engineered with various ligands for targeting to the desired subcellular compartments like cytosol, nucleus and mitochondria.…”

Section: Strategies For Intracellular Protein Deliverymentioning

confidence: 99%

Rational Design of Nanocarriers for Intracellular Protein Delivery

et al. 2019

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Protein/antibody therapeutics have exhibited the advantages of high specificity and activity even at an extremely low concentration compared to small molecule drugs. However, they are accompanied by unfavorable physicochemical properties such as fragile tertiary structure, large molecular size, and poor penetration of the membrane, and thus the clinical use of protein drugs is hindered by inefficient delivery of proteins into the host cells. To overcome the challenges associated with protein therapeutics and enhance their biopharmaceutical applications, various protein‐loaded nanocarriers with desired functions, such as lipid nanocapsules, polymeric nanoparticles, inorganic nanoparticles, and peptides, are developed. In this review, the different strategies for intracellular delivery of proteins are comprehensively summarized. Their designed routes, mechanisms of action, and potential therapeutics in live cells or in vivo are discussed in detail. Furthermore, the perspective on the new generation of delivery systems toward the emerging area of protein‐based therapeutics is presented as well.

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Protein delivery into cells using inorganic nanoparticle–protein supramolecular assemblies

Cited by 160 publications

References 53 publications

Surface chemistry of gold nanoparticles for health-related applications

Surface chemistry of gold nanoparticles for health-related applications

Nanocarrier‐Mediated Cytosolic Delivery of Biopharmaceuticals

Rational Design of Nanocarriers for Intracellular Protein Delivery

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