PEG Molecular Net-Cloth Grafted on Polymeric Substrates and Its Bio-Merits

Zhao, Changwen; Lin, Zhifeng; Yin, Huabing; Ma, Yuhong; Xu, Fu‐Jian; Yang, Wantai

doi:10.1038/srep04982

Cited by 21 publications

(20 citation statements)

References 35 publications

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“…In our previous work, we demonstrated that the surface-attached highly crosslinked PEG hydrogel exhibited a nonswelling property. 32 The hydrogel film prepared in the present work composed of 90% crosslinker (PEGDA) also is highly crosslinked and nonswelling, which can maintain stable spot shape and size during complex operation processes.…”

Section: Resultsmentioning

confidence: 96%

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Preparation of a poly (PEGDA‐co‐GMA) thin hydrogel matrix for oligonucleotide microarray applications

Yuan

Zhao

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND: Fabricating oligonucleotide microarrays with high immobilization efficiency and sensitivity by a simple surface modification route remains highly desirable in the biochip field. Here we develop a facile method to prepare a 3D crosslinking thin hydrogel film on glass slides for high-density immobilization of DNA probes. RESULTS: Thin hydrogel film using poly (ethylene glycol) diacrylate (PEGDA) as crosslinker and glycidyl methacrylate (GMA) as functional monomer was attached on vinyl group-modified glass slides by a photopolymerization technique. The effect of monomer ratios (PEGDA:GMA) on the surface hydrophilicity of the hydrogel layer and the immobilization density of DNA probes were investigated and an optimal monomer ratio of 10:1 and te maximum immobilization density of 1.4 pmol cm -2 were found. Adjusting the hydrophilicity of the hydrogel film effectively suppressed its swelling and produced a typical dense but not porous structure with a flat surface favorable for printing microarrays. The maximum immobilization density and efficiency of oligonucleotide probes on the 3D hydrogel film were 1.4 pmol cm -2 and 83%, respectively, which are superior values to those on a 1D support. The microarray had a wide detection range of target nucleotides from 15.3 pmol L -1 to 62.5 nmol L -1 . In addition, the hybridization assay demonstrated that the microarray showed high sensitivity with a limit of detection of 30.5 pmol L -1 . CONCLUSION:The poly (PEGDA-co-GMA) hydrogel film could be grafted on the glass slide by the photopolymerization technique and the DNA probe was effectively immobilized on the thin hydrogel by reacting with the epoxy groups. This strategy of attaching a thin cross-linked poly(PEGDA-co-GMA) hydrogel layer on glass slides has promising prospects in the fields of bioanalysis and microarrays.

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Section: Resultsmentioning

confidence: 96%

“…The absorption peak at 1722 cm −1 belongs to the C O stretching vibration of ester groups derived from PEGDA and GMA. 31,32 A new absorption band at 910 cm −1 appeared [Fig. 1 (a)], ascribed to the epoxy ring from GMA, 33 indicating that the epoxy groups have been successfully incorporated into the hydrogel film.…”

Section: Resultsmentioning

confidence: 99%

Preparation of a poly (PEGDA‐co‐GMA) thin hydrogel matrix for oligonucleotide microarray applications

Yuan

Zhao

et al. 2021

J of Chemical Tech & Biotech

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“…Conversely, the higher degree of crosslinking in PEGDA 700 results in lower swelling. 50,51 Due to effective adhesion between the two layers, this differential swelling caused the PEGDA 700/4000 bilayers to curve spontaneously. The magnitude of the radius of curvature is a result of the balance between this differential swelling and the bending rigidity of the composite structure-a property that is dependent upon the modulus and thickness of each of the layers.…”

Section: Resultsmentioning

confidence: 99%

A Self-Folding Hydrogel In Vitro Model for Ductal Carcinoma

Kwag

Serbo

Korangath

et al. 2016

Tissue Engineering Part C: Methods

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A significant challenge in oncology is the need to develop in vitro models that accurately mimic the complex microenvironment within and around normal and diseased tissues. Here, we describe a self-folding approach to create curved hydrogel microstructures that more accurately mimic the geometry of ducts and acini within the mammary glands, as compared to existing three-dimensional block-like models or flat dishes. The microstructures are composed of photopatterned bilayers of poly (ethylene glycol) diacrylate (PEGDA), a hydrogel widely used in tissue engineering. The PEGDA bilayers of dissimilar molecular weights spontaneously curve when released from the underlying substrate due to differential swelling ratios. The photopatterns can be altered via AutoCAD-designed photomasks so that a variety of ductal and acinar mimetic structures can be mass-produced. In addition, by co-polymerizing methacrylated gelatin (methagel) with PEGDA, microstructures with increased cell adherence are synthesized. Biocompatibility and versatility of our approach is highlighted by culturing either SUM159 cells, which were seeded postfabrication, or MDA-MB-231 cells, which were encapsulated in hydrogels; cell viability is verified over 9 and 15 days, respectively. We believe that self-folding processes and associated tubular, curved, and folded constructs like the ones demonstrated here can facilitate the design of more accurate in vitro models for investigating ductal carcinoma.

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“…In this study, an extremely simple and mild technique, called visible light induced graft polymerization [31][32][33], was used to immobilize two enzymes on polymer substrate separately and simultaneously. The mechanism of this technique composed of two chemical steps (Scheme 1).…”

Section: Introductionmentioning

confidence: 99%