Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Arora, Sanu; Steuernagel, Burkhard; Gaurav, Kumar; Chandramohan, Sutha; Long, Yunming; Matny, Oadi; Johnson, Ryan C.; Enk, Jacob; Periyannan, Sambasivam; Singh, Narinder; Hatta, Muhammad Asyraf Md; Athiyannan, Naveenkumar; Cheema, Jitender; Yu, Guotai; Kangara, Ngonidzashe; Ghosh, Sreya; Szabo, Les J.; Poland, Jesse; Bariana, Harbans; Jones, Jonathan D. G.; Bentley, Alison R.; Ayliffe, Mick; Olson, Eric; Xu, Steven S.; Steffenson, Brian J.; Lagudah, Evans; Wulff, Brande B. H.

doi:10.1038/s41587-018-0007-9

Cited by 264 publications

(223 citation statements)

References 57 publications

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“…Novel molecular approaches such as engineering “decoys” (12) and protein resurfacing, as described here, combined with modern transformation (36) and breeding pipelines (37), offers the opportunity for more targeted approaches to disease resistance breeding. These will complement other emerging technologies in NLR identification (38) and NLR stacking (4) as methods to develop improved crops for the future.…”

Section: Discussionmentioning

confidence: 99%

Protein engineering expands the effector recognition profile of a rice NLR immune receptor

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Franceschetti

Terauchi

et al. 2019

Preprint

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22Plant NLR receptors detect pathogen effectors and initiate an immune response. Since 23 their discovery, NLRs have been the focus of protein engineering to improve disease 24 resistance. However, this has proven challenging, in part due to their narrow response 25 specificity. Here, we used structure-guided engineering to expand the response 26 profile of the rice NLR Pikp to variants of the rice blast pathogen effector AVR-Pik. A 27 mutation located within an effector binding interface of the integrated Pikp-HMA 28 domain increased the binding affinity for AVR-Pik variants in vitro and in vivo. This 29 translates to an expanded cell death response to AVR-Pik variants previously 30 unrecognized by Pikp in planta. Structures of the engineered Pikp-HMA in complex 31 with AVR-Pik variants revealed the mechanism of expanded recognition. These 32 results provide a proof-of-concept that protein engineering can improve the utility of 33 plant NLR receptors where direct interaction between effectors and NLRs is 34 established, particularly via integrated domains. 35 36Protein engineering offers opportunities to develop new or improved molecular 37 recognition capabilities that have applications in basic research, health and 38 agricultural settings. Protein resurfacing, where the properties of solvent-exposed 39 regions are changed (often by mutation), has been used extensively in diverse areas 40 from antibody engineering for clinical use, to production of more stable, soluble 41 proteins for biotechnology applications (1). 42

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Section: Discussionmentioning

confidence: 99%

Protein engineering expands the effector recognition profile of a rice NLR immune receptor

Concepcion

Franceschetti

Terauchi

et al. 2019

Preprint

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“…Cataloging NLR gene diversity in plants is of interest for resistance gene discovery, for insight into NLR gene evolution, and for clues regarding the functions of IDs. Sequence capture by hybridization approaches, such as RenSeq, have been developed and applied to catalog NLR genes in representative varieties of several plant species [65–71], but these depend on a priori knowledge to design the capture probes and thus may miss structural variants. Also, they do not reveal genomic location, recent duplications, or arrangement of the genes, information necessary to investigate evolutionary patterns.…”

Section: Discussionmentioning

confidence: 99%

Genome assembly and characterization of a complex zfBED-NLR gene-containing disease resistance locus in Carolina Gold Select rice with Nanopore sequencing

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Moscou

Zimin³

et al. 2019

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“…(2016) applied MutRenSeq for isolation of two R genes ( Sr22 and Sr45 ) that confer stem rust resistance in wheat. More recently, a speed cloning approach using high‐throughput DNA sequencing (AgRenSeq) for NLR gene enrichment was reported to identify and isolate four wheat stem rust R genes from the wheat wild progenitor Aegilops tauschii (Arora et al , 2019), the D genome donor of bread or hexaploidy wheat. These state‐of‐the‐art genomic technologies effectively compensate for the reduced recombination during introgression of foreign sources of disease resistance to wheat (Wulff and Moscou, 2014), providing the way for fast‐track identification of resistance loci and the utilization of crop wild relatives.…”

Section: Advances In Genomics and High‐throughput Genotyping Technolomentioning

confidence: 99%

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

et al. 2020

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Powdery mildew (Blumeria graminis f. sp. tritici) results in serious economic loss in wheat production. Exploration of plant resistance to wheat powdery mildew over several decades has led to the discovery of a wealth of resistance genes and quantitative trait loci (QTLs). We have provided a comprehensive summary of over 200 powdery mildew genes (permanently and temporarily designated genes) and QTLs reported in common bread wheat. This highlights the diverse and rich resistance sources that exist across all 21 chromosomes. To manage different data for breeders, here we also present a bridged mapping result from previously reported powdery mildew resistance genes and QTLs with the application of a published integrated wheat map. This will provide important insights to empower further breeding of powdery mildew resistant wheat via marker‐assisted selection (MAS).

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Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Cited by 264 publications

References 57 publications

Protein engineering expands the effector recognition profile of a rice NLR immune receptor

Protein engineering expands the effector recognition profile of a rice NLR immune receptor

Genome assembly and characterization of a complex zfBED-NLR gene-containing disease resistance locus in Carolina Gold Select rice with Nanopore sequencing

Mechanisms of powdery mildew resistance of wheat – a review of molecular breeding

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