2012
DOI: 10.1038/mt.2012.69
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Abstract: In this study, we tested the hypothesis that DNA vaccination in the skin using microneedles improves protective immunity compared to conventional intramuscular (i.m.) injection of a plasmid DNA vaccine encoding the influenza hemagglutinin (HA). In vivo fluorescence imaging demonstrated the expression of a reporter gene delivered to the skin using a solid microneedle patch coated with plasmid DNA. Vaccination at a low dose (3 µg HA DNA) using microneedles generated significantly stronger humoral immune response… Show more

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Cited by 66 publications
(47 citation statements)
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“…The discrepancy might be explained by the current study employing only a single immunization, a low injection volume, and a low DNA dose. By contrast, previous studies by others describe protection first after several immunizations (6,7,10), larger volumes (7,9,10,56,57), and larger amounts of DNA (9,(56)(57)(58). Another factor that may explain the discrepancy is that we have used a truncated HA in which most of the transmembrane and intracellular parts have been deleted.…”
Section: Discussionmentioning
confidence: 63%
See 1 more Smart Citation
“…The discrepancy might be explained by the current study employing only a single immunization, a low injection volume, and a low DNA dose. By contrast, previous studies by others describe protection first after several immunizations (6,7,10), larger volumes (7,9,10,56,57), and larger amounts of DNA (9,(56)(57)(58). Another factor that may explain the discrepancy is that we have used a truncated HA in which most of the transmembrane and intracellular parts have been deleted.…”
Section: Discussionmentioning
confidence: 63%
“…However, the induction of adequate immune responses against influenza require large doses of DNA even in mice, and often in combination with adjuvants or several booster injections (4)(5)(6)(7)(8)(9)(10). Also, DNA vaccines often have a tendency of skewing immune responses toward T cell immunity, whereas Abs are of major importance in influenza prevention.…”
mentioning
confidence: 99%
“…DNA vaccine delivery to skin have been accomplished in the past using various methods such a tape-stripping, microdermabrasion, ballistic penetration, electroporation and microneedles to breach the skin barrier function [13][14][15][16][17][18][19][20][21][22][23][24] to allow DNA vaccines to diffuse within the skin into the APCs in the epidermis and dermis. Gene gun has been a popular method of delivering DNA vaccines to skin in the past [25][26][27][28][29][30].…”
Section: Introductionmentioning
confidence: 99%
“…There are several types of microneedle delivery systems, including array-type microneedles, coated with antigen, or a single hollow microneedle attached to an injector device that dispenses liquid antigen (121). Microneedles have shown promise in the delivery of vaccines and are reportedly easy to use, painless, and effective, even improving the immunogenicity of DNA vaccines (122). Intanza (or FluzoneIntradermal in the United States), marketed by Sanofi Pasteur, which uses the hollow-type microneedles attached to a microinjection system with a lower dose of HA, has been licensed for use in the elderly in several countries (123)(124)(125) and in the United States since 2011 (Table 1).…”
Section: Route Of Vaccine Deliverymentioning
confidence: 99%