Designed Signaling Aptamers that Transduce Molecular Recognition to Changes in Fluorescence Intensity

Jhaveri, Sulay D.; Kirby, Romy; Conrad, Rick; Maglott, Emily J.; Bowser, Michael T.; Kennedy, Robert T.; Glick, Gary D.; Ellington, Andrew D.

doi:10.1021/ja992393b

Cited by 271 publications

(237 citation statements)

References 20 publications

(35 reference statements)

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“…Combining the aptamer affinity and easy signal transduction aptamer-based sensors have attracted wide and increasing interest [2][3][4]. In the aptamer-based sensors, the socalled signaling aptamers rely on the specific recognition of the targets by the aptamers and the binding-induced signal generations [5][6][7][8]. The signal responses of the signaling aptamers are unique properties of the aptamers.…”

Section: Introductionmentioning

confidence: 99%

“…The signal responses of the signaling aptamers are unique properties of the aptamers. The generated signals can be electrochemical, colorimetric, or fluorescent output [3][4][5][6][7][8]. By using fluorophores on aptamers, the fluorescent signaling aptamers exhibited benefits in sensitivity, simplicity, convenience of detection, diverse detection approaches, and usual homogeneous binding process [5][6][7][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…The generated signals can be electrochemical, colorimetric, or fluorescent output [3][4][5][6][7][8]. By using fluorophores on aptamers, the fluorescent signaling aptamers exhibited benefits in sensitivity, simplicity, convenience of detection, diverse detection approaches, and usual homogeneous binding process [5][6][7][9][10][11][12][13][14]. Usually, fluorescent dyes can be readily introduced on the ends of the aptamers through chemical synthesis to fabricate the aptamer switch [5][6][7][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…By using fluorophores on aptamers, the fluorescent signaling aptamers exhibited benefits in sensitivity, simplicity, convenience of detection, diverse detection approaches, and usual homogeneous binding process [5][6][7][9][10][11][12][13][14]. Usually, fluorescent dyes can be readily introduced on the ends of the aptamers through chemical synthesis to fabricate the aptamer switch [5][6][7][9][10][11][12][13][14]. It is also feasible to conjugate fluorophores on the internal bases or to use the fluorescent analogue of the bases in oligonucleotides for sensing [5][6][7][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer

2013

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We explored a fluorescent strategy for sensing ochratoxin A (OTA) by using a single fluorophore-labeled aptamer for detection of OTA. This method relied on the change of the fluorescence intensity of the labeled dye induced by the specific binding of the fluorescent aptamer to OTA. Different fluorescein labeling sites of aptamers were screened, including the internal thymine bases, 3′-end, and 5′-end of the aptamer, and the effect of the labeling on the aptamer affinity was investigated. Some fluorophorelabeled aptamers showed a signal-on or signal-off response. With the fluorescent aptamer switch, simple, rapid, and selective sensing of OTA at nanomolar concentrations was achieved. OTA spiked in diluted red wine could be detected, showing the feasibility of the fluorescent aptamer for a complex matrix. This method shows potential for designing aptamer sensors for other targets.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer

2013

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“…Among the various signal transduction methods, fluorescence has been the most often used due to its high sensitivity and versatility. 5,[20][21][22][23][24][25][26][27] In addition to intensity-based detection, fluorescence wavelength shift, anisotropy, lifetime, and energy transfer have all been reported for designing aptamer-based sensors. 5 Most fluorescent aptamer sensors involve cuvette-based measurements, in which the sensor and target analyte are mixed in a cuvette and the change of fluorescence signal is monitored with a fluorometer.…”

Section: Introductionmentioning

confidence: 99%

Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor

Huang

Liu

2010

Anal. Chem.

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Aptamers are single-stranded nucleic acids that can selectively bind to essentially any molecule of choice. Because of their high stability, low cost, ease of modification and availability through selection, aptamers hold great promise in addressing key challenges in bioanalytical chemistry. In the past fifteen years many highly sensitive fluorescent aptamer sensors have been reported. However, few such sensors showed high performance in serum samples. Further challenges related to practical applications include detection in a very small sample volume and a low dependence of sensor performance on ionic strength.We report the immobilization of an aptamer sensor on a magnetic microparticle and the use of flow cytometry for detection. Flow cytometry allows the detection of individual particles in a capillary and can effectively reduce the light scattering effect of serum. Since DNA immobilization generated a highly negatively charged surface and caused an enrichment of counter ions, the sensor performance showed a lower salt dependence. The detection limits for adenosine are determined to be 178 and 167 M in buffer and in 30% serum, respectively. Finally, we demonstrated that the detection can be carried out in 10 L of 90% human blood serum.3

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Aptameric Biosensors

Meir

Marks

Stojanović

2007

Handbook of Biosensors and Biochips

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Aptamers are synthetic single stranded DNA or RNA molecules that can bind with high affinity and specificity to their non‐nucleic acid target molecules. The artificial nucleic acid ligands can be generated against proteins, small molecules, ions, whole cells, tissues and organs. The process of aptamers isolation, their affinity properties and applications thereof are described. In addition, the particular case of how structural changes in aptamers can be used to construct fluorescent molecular sensors coupling both recognition and signaling properties is explained and their use finally as recognition elements in various platforms of biosensors are herein illustrated.

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Designed Signaling Aptamers that Transduce Molecular Recognition to Changes in Fluorescence Intensity

Cited by 271 publications

References 20 publications

Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer

Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer

Flow Cytometry-Assisted Detection of Adenosine in Serum with an Immobilized Aptamer Sensor

Aptameric Biosensors

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