Luminescence Resonance Energy Transfer

Selvin, Paul R.; Rana, Tariq M.; Hearst, John E.

doi:10.1021/ja00092a088

Cited by 201 publications

(177 citation statements)

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“…These lifetime data are consistent with data recorded for analogous lanthanide systems such as europium chelates and Cy5 organic dye, suggesting that the fast decay component may be related to intramolecular energy transfer while the long component is related to the nanoparticle donor only [36]. In addition to lack of an AF fluorescence peak from the detection complex in the absence of okadaic acid, no shortening of luminescence lifetimes was noted for the nanoparticles bound only to antibodies or for nanoparticles bound to F ab -AF labelled antibodies.…”

Section: Photoluminescence and Fluorescence Spectrasupporting

confidence: 88%

Antibody-based donor–acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin

Stipić

Burić

Jakšić

et al. 2015

Colloids and Surfaces B: Biointerfaces

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With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore : nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37-3.97 μM okadaic acid range with a limit of detection of 0.25 μM. This work may have important implications for the development of new, cheap and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.

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Section: Photoluminescence and Fluorescence Spectrasupporting

confidence: 88%

Antibody-based donor–acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin

Stipić

Burić

Jakšić

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…111 Some of the most striking systems in terms of architecture design, easy synthesis, and multifunctional applications 122 are summarized below. Lanthanide chelates have been studied since the 1990s [123][124][125] as extremely promising luminescence probes for distance measurements using luminescence resonance energy transfer techniques. Chelated lanthanide labels offer enhancement of the molar absorption coefficients of the lanthanides by the donor units, as already mentioned (see Fig.…”

Section: Lanthanide-doped Hybrid Materials As Sensors In Biological Mmentioning

confidence: 99%

Photonic and nanobiophotonic properties of luminescent lanthanide-doped hybrid organic–inorganic materials

et al. 2008

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Research into lanthanide-doped organic-inorganic hybrid materials emerged in the 1990s with the development of interesting materials for optics: high efficiency and stable solid-state lasers, new fiber amplifiers and sensors, devices with upconversion, fast photochromic and non-linear responses, etc. Their interest relies on the possibility of combining properties of sol-gel host materials (shaping, tunable refractive index and mechanical properties, corrosion protection, specific adhesion, etc.) and the well-known luminescence of lanthanide ions (Ln). The fast development of photonic hybrids allowed the commercial exploitation of products with new or enhanced characteristics (megajoule pulsed Nd-YAG laser, protective coatings of glasses, screens or glasswares). However, recently, Ln-hybrid nanocomposites have found new applications in bio-sensors, bio-analytics and even clinical imaging diagnostics. These applications make use of the fluorescence properties of lanthanides that make luminescent hybrids ideal candidates for time-resolved fluoroimmunoassays, DNA hybridation assays, fluorescence imaging microscopy, or in vivo imaging. As a consequence, the goal of this review is twofold: (i) as a reminder of some general considerations that must be taken into account to design new optically active Ln-doped nanocomposites whatever the application field, and (ii) to show the most important advances achieved in the past years in different areas, paying special attention to bio-medical applications.

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“…The R 0 was calculated assuming a completely randomized orientations of donor and acceptor. This assumption is particularly valid in the case of long-lived europium chelate donors (47)(48)(49) and practically eliminates the uncertainty of distance measurement due to unknown mobility of fluorochromes.…”

Section: Architecture Of the Hmgi/y⅐dna Complexesmentioning

confidence: 99%

Distinct Organization of DNA Complexes of Various HMGI/Y Family Proteins and Their Modulation upon Mitotic Phosphorylation

Piekiełko¹,

Drung²,

Rogalla³

et al. 2001

Journal of Biological Chemistry

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High mobility group (HMG) proteins HMGI, HMGY, HMGI-C, and Chironomus HMGI are DNA-binding proteins thought to modulate the assembly and the function of transcriptional complexes. Each of these proteins contains three DNA-binding domains (DBD), properties of which appear to be regulated by phosphorylation. High levels of these proteins are characteristic for rapidly dividing cells in embryonic tissues and tumors. On the basis of their occurrence, specific functions for each of these proteins have been postulated. In this study we demonstrate differences in the nature of contacts of these proteins with promoter region of the interferon-␤ gene. We show that HMGI and HMGY interact with this DNA via three DBDs, whereas HMGI-C and Chironomus HMGI bind to this DNA using only two domains. Phos- Despite continuously increasing interest in the function(s) of this group of proteins, the nature of the interaction of these proteins with DNA is still not sufficiently understood. The proteins of the HMGI/Y family are 10 -11 kDa in size, are highly charged, are rich both in acidic and basic residues, are proline-rich, and contain only few residues with bulky hydrophobic side chains. This unusual amino acid composition inhibits folding of the polypeptide backbone of these proteins into any defined secondary structure. Common for HMGI/Y proteins is the presence of three putative DNA-binding domains (DBD), so called . NMR analysis of a complex of a peptide derived from HMGI(Y) bound to a short DNA fragment revealed that the centrally located RGR residues are essentially for binding and responsible for contacts of the protein with the bases and phosphate-sugar backbone (27). Alternative approaches, which used deletion and point-mutated proteins, revealed that two or three DBDs of the protein bind to DNA in a cooperative way (28 -30). Application of the protein-footprinting method for mapping of protein regions interacting with DNA (31) allowed more detailed characterization of the binding of HMGI(Y) proteins to DNA (32). A combination of this method

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Luminescence Resonance Energy Transfer

Cited by 201 publications

References 0 publications

Antibody-based donor–acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin

Antibody-based donor–acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin

Photonic and nanobiophotonic properties of luminescent lanthanide-doped hybrid organic–inorganic materials

Distinct Organization of DNA Complexes of Various HMGI/Y Family Proteins and Their Modulation upon Mitotic Phosphorylation

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