High-Performance Three-Dimensional Tubular Nanomembrane Sensor for DNA Detection

Abstract

We report an ultrasensitive label-free DNA biosensor with fully on-chip integrated rolled-up nanomembrane electrodes. The hybridization of complementary DNA strands (avian influenza virus subtype H1N1) is selectively detected down to attomolar concentrations, an unprecedented level for miniaturized sensors without amplification. Impedimetric DNA detection with such a rolled-up biosensor shows 4 orders of magnitude sensitivity improvement over its planar counterpart. Furthermore, it is observed that the impedance response of the proposed device is contrary to the expected behavior due to its particular geometry. To further investigate this difference, a thorough model analysis of the measured signal and the electric field calculation is performed, revealing enhanced electron hopping/tunneling along the DNA chains due to an enriched electric field inside the tube. Likewise, conformational changes of DNA might also contribute to this effect. Accordingly, these highly integrated three-dimensional sensors provide a tool to study electrical properties of DNA under versatile experimental conditions and open a new avenue for novel biosensing applications (i.e., for protein, enzyme detection, or monitoring of cell behavior under in vivo like conditions).

Assembly and Self‐Assembly of Nanomembrane Materials—From 2D to 3D

“…Its performance (characterized by electrochemical impedance spectroscopy) is demonstrated in panel (ii) of Figure d, where performance of planar device is also shown for comparison. For the planar device, impedance value increases with increasing DNA concentration, whereas for the rolled‐up device impedance value decreases . The charge transfer resistance R CT is very sensitive to the variation of DNA concentration in the a m –p m range for rolled‐up device, while the planar device is sensitive only in the p m range (panel (ii) of Figure d).…”

“…The metal stripes rolled with nanomembrane may also be used to transmit electrical signals and thus, complex 3D electrical devices can be obtained . The electrical device may be further incorporated with microfluidic functions to achieve a multifunctional sensor.…”

Whispering Gallery Mode Optical Microresonators: Structures and Sensing Applications

“…Previous researchers used the hollow characteristics of microtubulars and combined them with microfluidic channels, which laid the foundation for unmarked detection with good selectivity and specificity . In 2007, Suter et al.…”

Advanced nanotechnologies in avian influenza: Current status and future trends – A review

“…Together with quartz crystal microbalance (QCM) aptasensors, the magnetic nanobead-amplified QCM immunosensors have been used to detect the H5N1 protein [38]. In addition to these NPs, some other nanomaterialbased techniques, including nanowell array electrode-based electrochemical quantitative systems [44] and fully integrated on-chip tubular nanomembrane sensors [45], have also been developed to efficiently detect viral DNA. Many of these particles are commercially available and/or were prepared using published procedures, with some modifications [39e42].…”