Effects of electron transport inhibitors on iron reduction in Aeromonas hydrophila strain KB1

Woźnica, Andrzej; Dzirba, J; Mańka, D; Labuzek, S.

doi:10.1016/s1075-9964(03)00059-3

Cited by 30 publications

(16 citation statements)

References 21 publications

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“…The mitochondrial electron transport chain has been implicated in the reduction and intracellular transport of Fe 3ϩ in bacteria (32). Our data demonstrate that multiple inhibitors of the electron transport chain decrease iron transport in HBE cells.…”

Section: L278 Dcytb: a Novel Ferrireductase In The Lungmentioning

confidence: 54%

Duodenal cytochrome b: a novel ferrireductase in airway epithelial cells

Turi¹,

Wang²,

McKie³

et al. 2006

American Journal of Physiology-Lung Cellular and Molecular Phys

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Catalytically active iron in the lung causes oxidative stress and promotes microbial growth that can be limited by intracellular sequestration of iron within ferritin. Because cellular iron uptake requires membrane ferrireductase activity that in the gut can be provided by duodenal cytochrome b (Dcytb), we sought Dcytb in the lung to test the hypothesis that it contributes to epithelial iron regulation by reducing Fe(3+) for cellular iron transport. Dcytb expression was found in respiratory epithelium in vitro and in vivo and was responsive to iron concentration. Iron transport was measured in human bronchial epithelial (HBE) cells using inductively coupled plasma atomic emission spectroscopy and was demonstrated to be partially inhibited in the presence of Dcytb-blocking antibody, suggesting that Dcytb reduces Fe(3+) for cellular iron transport. A definite source of reducing equivalents for Dcytb was sought but not identified. We found no evidence that ascorbate was involved but did demonstrate that O(2)(-). production decreased when Dcytb function was blocked. The presence of Dcytb in airway epithelial cells and its regulation by iron therefore may contribute to pulmonary cytoprotection.

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Section: L278 Dcytb: a Novel Ferrireductase In The Lungmentioning

confidence: 54%

Duodenal cytochrome b: a novel ferrireductase in airway epithelial cells

Turi¹,

Wang²,

McKie³

et al. 2006

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…Inhibitors of the electron transport chain or ATP synthase and uncouplers of the periplasmic membrane u s e d i n t h i s s t u d y i n c l u d e d D C C D ( N , N ′-dicyclohexylcarbodiimide) (dissolved in acetone), dicumarol (dissolved in 0.5 mmol L −1 NaOH), quinacrine (dissolved in H 2 O), and CCCP (carbonyl-cyanide-m-chlorophenyl hydrazine)/antimycin A/capsaicin (dissolved in 95 % ethanol) (Knight and Blakemore 1998;Woznica et al 2003). Any inhibition effect was expressed as the percentage inhibition in electron transport to different electron acceptors (i.e., percent decrease in reduction product) compared with that without inhibitor (control) (Knight and Blakemore 1998;Woznica et al 2003). When oxygen was the electron acceptor, percentage inhibition was calculated as the percent decrease in microbial aerobic growth, determined by OD 600 nm .…”

Section: Inhibition Experimentsmentioning

confidence: 99%

“…7). Inhibition sites of the seven respiratory and ATP synthase inhibitors and uncouplers used are shown in Fig. 7a (Knight and Blakemore 1998;Woznica et al 2003): capsaicin and rotenone inhibit the activity of NADH dehydrogenase, quinacrine inhibits the activity of FAD dehydrogenase, dicumarol is an inhibitor of the quinone loop, antimycin A inhibits the cytochrome b/c complex, DCCD acts as an inhibitor of ATP synthase, and CCCP dissipates the proton motive force (PMF).…”

Section: Preliminary Models For Electron Transport Pathways With Varimentioning

confidence: 99%

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Anaerobic humus and Fe(III) reduction and electron transport pathway by a novel humus-reducing bacterium, Thauera humireducens SgZ-1

et al. 2014

Appl Microbiol Biotechnol

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In this study, an anaerobic batch experiment was conducted to investigate the humus- and Fe(III)-reducing ability of a novel humus-reducing bacterium, Thauera humireducens SgZ-1. Inhibition tests were also performed to explore the electron transport pathways with various electron acceptors. The results indicate that in anaerobic conditions, strain SgZ-1 possesses the ability to reduce a humus analog, humic acids, soluble Fe(III), and Fe(III) oxides. Acetate, propionate, lactate, and pyruvate were suitable electron donors for humus and Fe(III) reduction by strain SgZ-1, while fermentable sugars (glucose and sucrose) were not. UV-visible spectra obtained from intact cells of strain SgZ-1 showed absorption peaks at 420, 522, and 553 nm, characteristic of c-type cytochromes (cyt c). Dithionite-reduced cyt c was reoxidized by Fe-EDTA and HFO (hydrous ferric oxide), which suggests that cyt c within intact cells of strain SgZ-1 has the ability to donate electrons to extracellular Fe(III) species. Inhibition tests revealed that dehydrogenases, quinones, and cytochromes b/c (cyt b/c) were involved in reduction of AQS (9, 10-anthraquinone-2-sulfonic acid, humus analog) and oxygen. In contrast, only NADH dehydrogenase was linked to electron transport to HFO, while dehydrogenases and cyt b/c were found to participate in the reduction of Fe-EDTA. Thus, various different electron transport pathways are employed by strain SgZ-1 for different electron acceptors. The results from this study help in understanding the electron transport processes and environmental responses of the genus Thauera.

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“…For instance, NADH/ubiquinone (UQ) oxidoreductase can be inhibited by rotenone, capsaicin and dicumarol [9]–[11] and flavin oxidoreductase by quinacrine [12]. The latter, being an isoalloxazine analog, may also act as a competitive inhibitor of molybdopteridin cofactors [13]. Another group comprises inhibitors of cytochrome c oxidase aa3: cyanide, azide and carbon monoxide [14].…”

Section: Introductionmentioning

confidence: 99%

Stimulatory Effect of Xenobiotics on Oxidative Electron Transport of Chemolithotrophic Nitrifying Bacteria Used as Biosensing Element

et al. 2013

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Electron transport chain (ETCh) of ammonium (AOB) and nitrite oxidizing bacteria (NOB) participates in oxidation of ammonium to nitrate (nitrification). Operation of ETCh may be perturbed by a range of water-soluble xenobiotics. Therefore, consortia of nitrifying bacteria may be used as a biosensor to detect water contamination. A surprising feature of this system is an increase of oxygen consumption, detected in the presence of certain inhibitors of ETCh. Thus, to shed light on the mechanism of this effect (and other differences between inhibitors) we monitored separately respiration of the bacteria of the first (AOB - Nitrosomonas) and second (NOB -Nitrobacter) stages of nitrification. Furthermore, we measured plasma membrane potential and the level of reduction of NAD(P)H. We propose a novel model of ETCh in NOB to explain the role of reverse electron transport in the stimulation of oxygen consumption (previously attributed to hormesis).

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Effects of electron transport inhibitors on iron reduction in Aeromonas hydrophila strain KB1

Cited by 30 publications

References 21 publications

Duodenal cytochrome b: a novel ferrireductase in airway epithelial cells

Duodenal cytochrome b: a novel ferrireductase in airway epithelial cells

Anaerobic humus and Fe(III) reduction and electron transport pathway by a novel humus-reducing bacterium, Thauera humireducens SgZ-1

Stimulatory Effect of Xenobiotics on Oxidative Electron Transport of Chemolithotrophic Nitrifying Bacteria Used as Biosensing Element

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