2002
DOI: 10.1016/s0960-9822(02)00976-4
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Abstract: We conclude that DNA methylation does not control the methylation of histone H3-K9. We propose that loss of H3-K9 methylation is due to transcriptional reactivation, coupled with deposition of unmethylated nucleosomes. These findings are consistent with recent observations of DNA replication-independent deposition of histone H3.3 in Drosophila. Our results also suggest that, in Arabidopsis, DNA methylation is sufficient for gene silencing, but H3-K9 methylation is not.

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Cited by 426 publications
(96 citation statements)
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“…Increased H3K9me2 at FLC induced by vernalization suggested the involvement of a histone methyltransferase activity in vernalization (11). A strong candidate was KYP (18), which has been shown to regulate H3K9me2 levels of the transposons Ta2 and Ta3, the silenced floral repressor FWA, the zinc finger floral regulator SUPERMAN (40), and chromocenters (32). Mutants impaired in the H3K9 histone methyltransferase activity at FLC would share properties of vrn mutants.…”
Section: Discussionmentioning
confidence: 99%
“…Increased H3K9me2 at FLC induced by vernalization suggested the involvement of a histone methyltransferase activity in vernalization (11). A strong candidate was KYP (18), which has been shown to regulate H3K9me2 levels of the transposons Ta2 and Ta3, the silenced floral repressor FWA, the zinc finger floral regulator SUPERMAN (40), and chromocenters (32). Mutants impaired in the H3K9 histone methyltransferase activity at FLC would share properties of vrn mutants.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, the HMT Su(var)3-9 may be directly or indirectly associated with DNMT1. Interestingly, an Arabidopsis HMT contains a chromodomain (Johnson et al, 2002). Such domains in other proteins bind directly to methylated histones (Bannister et al, 2001;Lachner et al, 2001;Nakayama et al, 2001).…”
Section: Discussionmentioning
confidence: 99%
“…Unlike acetylation, however, methylation may not be easily reversed, as no demethylase activities have yet been identified. Intriguingly, histone methyltransferase (HMTs) activities in Neurospora crassa (Bird, 2001;Tamaru and Selker, 2001) and Arabidopsis (Johnson et al, 2002;Lachner, 2002) appear to direct DNA methylation, further suggesting that H3 methylation plays a central role in the establishment of stable silent states. Methylation of K9 in H3 is a very early event during X-inactivation that precedes CpG methylation, further indicating that H3 K9 methylation may direct heterochromatin formation (Heard et al, 2001;Mermoud et al, 2002).…”
Section: Introductionmentioning
confidence: 99%
“…Immunoprecipitations were performed by using a ChIP assay kit (Millipore, catalog no. 17-295) following the manufacturer's suggested protocol modified as previously described (42). Antibodies against trimethyl H3K9 and trimethyl H3K27 were obtained from Upstate Biotechnology.…”
Section: Rt-pcrmentioning
confidence: 99%