DNA gyrase inhibitory and antibacterial activity of some flavones(1)

Ohemeng, Kwasi A.; Schwender, Charles F.; Fu, Kwung P.; Barrett, John

doi:10.1016/s0960-894x(01)80881-7

Cited by 173 publications

(101 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast to V. harveyi, growth of C. freundii, E. coli, K. pneumoniae, P. vulgaris, S. enterica and S. marcescens was unaffected or only slightly affected by all tested compounds (Table 1). Our results obtained for E. coli, K. pneumoniae and P. vulgaris corroborate conclusions reported by some authors (Miski et al 1983;Ohemeng et al 1993) but are in contrast to results described by others (Oksuz et al 1984;Bashir et al 1994;Aljancic et al 1999). The individual bacteria revealed various degrees of sensitivity to different flavonoids.…”

Section: Resultssupporting

confidence: 91%

Assessment of antibacterial effects of flavonoids by estimation of generation times in liquid bacterial cultures

et al. 2007

View full text Add to dashboard Cite

Antibacterial activities of various flavonoids, a group of natural plant substances, have been reported previously, however, there are contradictory data, published by various authors, regarding sensitivity of particular bacterial species to these compounds. These problems arose apparently because of using different methods by various researchers. Here we tested sensitivity of several bacterial species (Gram-positive: Bacillus subtilis, Micrococcus luteus, Sarcina sp. and Staphylococcus aureus; and Gram-negative: Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enterica, Serratia marcescens and Vibrio harveyi) to various flavonoids: genistein and daidzein (isoflavones), apigenin (a flavone), naringenin (a flavanone) and kaempferol (a flavonol) by measurement of generation times of bacteria in liquid cultures. The presented results indicate that this simple method is adequate for unambiguous assessment of sensitivity of bacterial strains to flavonoids.

show abstract

Section: Resultssupporting

confidence: 91%

Assessment of antibacterial effects of flavonoids by estimation of generation times in liquid bacterial cultures

et al. 2007

View full text Add to dashboard Cite

show abstract

“…23) Ohemeng et al reported inhibitory activity of 14 flavonoids including kaempferol against E. coli DNA gyrase. 24) However, kaempferol itself did not show anti-MRSA activity (Table 1). Thus, it is likely that after C2 and C3 get into S. aureus cells and that these compounds, or their cleavage product kaempferol, or kaempferol 3-O-rhamnoside, inhibit DNA gyrase and topoisomerase IV.…”

Section: Discussionmentioning

confidence: 97%

Anti-methicillin Resistant Staphylococcus aureus (MRSA) Compounds Isolated from Laurus nobilis

Otsuka

Liu

Ogawa

et al. 2008

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

We found that an extract from Laurus nobilis L. (Lauraceae) leaves showed antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). We purified two flavonoids as the effective compounds and identified them as kaempferol 3-O-a a-L-(2؆,4؆-di-E-p-coumaroyl)-rhamnoside (C2) and kaempferol 3-O-a a-L-(2؆-Zp-coumaroyl-4؆-E-p-coumaroyl)-rhamnoside (C3). Both compounds showed strong antibacterial activity not only against MRSA but also against vancomycin-resistant enterococci (VRE). There was low or no antibacterial activity of C2 and C3 for Streptococcus pneumoniae, Pseudomonas aeruginosa and Serratia marcescens.

show abstract

“…It was discovered to possess anti-inflammatory activity and proved to be a phytoalexin (Nakazato et al 2000;Hernandez et al 2007). In addition, all these three flavonoids were reported to exhibit antibacterial activity, probably by acting on multiple cellular targets (Konstantinopoulou et al 2003;Cushnie and Lamb 2005), such as DNA gyrase (Ohemeng et al 1993) and cytoplasmic membrane (Mirzoeva et al 1997). Recently, quercetin and apigenin were found to inhibit some enzymes involved in the type II fatty acid biosynthesis (FAS II) pathway.…”

mentioning

confidence: 99%

Three flavonoids targeting the β‐hydroxyacyl‐acyl carrier protein dehydratase from Helicobacter pylori: Crystal structure characterization with enzymatic inhibition assay

et al. 2008

View full text Add to dashboard Cite

Flavonoids are the major functional components of many herbal and insect preparations and demonstrate varied pharmacological functions including antibacterial activity. Here by enzymatic assay and crystal structure analysis, we studied the inhibition of three flavonoids (quercetin, apigenin, and (S)-sakuranetin) against the b-hydroxyacyl-acyl carrier protein dehydratase from Helicobacter pylori (HpFabZ). These three flavonoids are all competitive inhibitors against HpFabZ by either binding to the entrance of substrate tunnel B (binding model A) or plugging into the tunnel C near the catalytic residues (binding model B) mainly by hydrophobic interaction and hydrogen-bond pattern. Surrounded by hydrophobic residues of HpFabZ at both positions of models A and B, the methoxy group at C-7 of (S)-sakuranetin seems to play an important role for the inhibitor's binding to HpFabZ, partly responsible for the higher inhibitory activity of (S)-sakuranetin than those of quercetin and apigenin against HpFabZ (IC 50 in mM: (S)-sakuranetin, 2.0 6 0.1; quercetin: 39.3 6 2.7; apigenin, 11.0 6 2.5). Our work is expected to supply useful information for understanding the potential antibacterial mechanism of flavonoids.Keywords: HpFabZ; quercetin; apigenin; (S)-sakuranetin; complex structure; inhibitory mechanism Flavonoids, with the largest and most important group of polyphenolic compounds in plants, are major functional components of many herbal and insect preparations for medical use (Havsteen 2002;Cushnie and Lamb 2005 Abbreviations: FabZ, b-hydroxyacyl-acyl carrier protein dehydratase; HpFabZ, Helicobacter pylori FabZ; FAS, fatty acid biosynthesis; PfFabZ, Plasmodium falciparum FabZ.Article and publication are at

show abstract

DNA gyrase inhibitory and antibacterial activity of some flavones(1)

Cited by 173 publications

References 8 publications

Assessment of antibacterial effects of flavonoids by estimation of generation times in liquid bacterial cultures

Assessment of antibacterial effects of flavonoids by estimation of generation times in liquid bacterial cultures

Anti-methicillin Resistant Staphylococcus aureus (MRSA) Compounds Isolated from Laurus nobilis

Three flavonoids targeting the β‐hydroxyacyl‐acyl carrier protein dehydratase from Helicobacter pylori: Crystal structure characterization with enzymatic inhibition assay

Contact Info

Product

Resources

About