The Zebrafish as a Model Organism to Study Development of the Immune System

Traver, David; Herbomel, Philippe; Patton, E. Elizabeth; Murphey, Ryan D.; Yoder, Jeffrey A.; Litman, Gary W.; Catic, André; Amemiya, Chris T.; Zon, Leonard I.; Trede, Nikolaus S.

doi:10.1016/s0065-2776(03)81007-6

Cited by 176 publications

(169 citation statements)

References 240 publications

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“…These macrophages express members of the evolutionarily conserved Toll-like family (zfTLR3, zflRAK-4, and zfTRAF6) which is of relevance for the innate immune system. Resembling our findings in Tupaia belangeri, primitive macrophages invade the zebrafish brain prior to its vascularization (for a review, see Traver et al, 2003). However, whether macrophage colonization of the brain follows the same principle in mammals and birds remains a matter of debate.…”

Section: Cerebellummentioning

confidence: 71%

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>

Knabe¹,

Washausen²

2015

Primate Biol.

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Abstract. The longstanding debate on the taxonomic status of Tupaia belangeri (Tupaiidae, Scandentia, Mammalia) has persisted in times of molecular biology and genetics. But way beyond that Tupaia belangeri has turned out to be a valuable and widely accepted animal model for studies in neurobiology, stress research, and virology, among other topics. It is thus a privilege to have the opportunity to provide an overview on selected aspects of neural development and neuroanatomy in Tupaia belangeri on the occasion of this special issue dedicated to Hans-Jürg Kuhn. Firstly, emphasis will be given to the optic system. We report rather "unconventional" findings on the morphogenesis of photoreceptor cells, and on the presence of capillary-contacting neurons in the tree shrew retina. Thereafter, network formation among directionally selective retinal neurons and optic chiasm development are discussed. We then address the main and accessory olfactory systems, the terminal nerve, the pituitary gland, and the cerebellum of Tupaia belangeri. Finally, we demonstrate how innovative 3-D reconstruction techniques helped to decipher and interpret so-far-undescribed, strictly spatiotemporally regulated waves of apoptosis and proliferation which pass through the early developing forebrain and eyes, midbrain and hindbrain, and through the panplacodal primordium which gives rise to all ectodermal placodes. Based on examples, this paper additionally wants to show how findings gained from the reported projects have influenced current neuroembryological and, at least partly, medical research.

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Section: Cerebellummentioning

confidence: 71%

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>

Knabe¹,

Washausen²

2015

Primate Biol.

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“…Furthermore, it allows for distinguishing between this arm of immunity and a combined innate and adaptive immune response in the context of infection, like in adult fish, which have a complex adaptive immune system akin to that of mammals ( Fig. 1) (Traver et al 2003;Meeker and Trede 2008;Renshaw and Trede 2012;van der Vaart et al 2012). The finalized whole-genome sequence of zebrafish (Howe et al 2013) reveals that 70% of human genes have at least one obvious zebrafish ortholog.…”

Section: General Properties Of Zebrafishmentioning

confidence: 99%

Animal Models of Tuberculosis: Zebrafish

Leeuwen

Sar

Bitter

2014

Cold Spring Harbor Perspectives in Medicine

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Correspondence: a.vandersar@vumc.nl Over the past decade the zebrafish (Danio rerio) has become an attractive new vertebrate model organism for studying mycobacterial pathogenesis. The combination of mediumthroughput screening and real-time in vivo visualization has allowed new ways to dissect host pathogenic interaction in a vertebrate host. Furthermore, genetic screens on the host and bacterial sides have elucidated new mechanisms involved in the initiation of granuloma formation and the importance of a balanced immune response for control of mycobacterial pathogens. This article will highlight the unique features of the zebrafish-Mycobacterium marinum infection model and its added value for tuberculosis research.

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“…19 Many zebrafish blood mutants identified in these screens are embryonic lethal, and we have recently described embryonic HCT to address issues of cell autonomous mutant gene function. 20 Similar transplantation of whole kidney marrow (WKM) cells into unconditioned adult recipients resulted in the disappearance of donor-derived cells within several weeks of transplantation.…”

Section: Introductionmentioning

confidence: 99%

Effects of lethal irradiation in zebrafish and rescue by hematopoietic cell transplantation

Traver

Winzeler

Stern

et al. 2004

Blood

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163

143

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The study of hematopoiesis has been greatly facilitated by transplantation of blood cell populations into recipient animals. Efficient engraftment of donor cells generally requires ablation of the host hematopoietic system. The zebrafish has recently emerged as a developmental and genetic system to study hematopoiesis. To enable the study of hematopoietic stem cell ( IntroductionThe study of the biologic effects of ␥ irradiation began over a century ago, 1 but it was not until deployment of the atomic bomb in 1945 that the clinical manifestations of lethal total body irradiation (TBI) were fully realized. Acute irradiation injury was replicated using animal models, and seminal experiments by Jacobsen et al 2,3 and Lorenz et al 4 showed that shielding or transplantation of hematopoietic tissues was sufficient for radioprotection of otherwise lethal doses. Although humoral factors produced from these tissues were initially proposed to be the radioprotective elements, 2 subsequent studies showed that survival correlated with the level of hematopoietic repopulation by donor-derived cells. [5][6][7][8] Pioneering studies by Till and McCulloch and colleagues showed that donor bone marrow contained rare cells that generated clonal, macroscopic spleen colonies, 9,10 a fraction of which regenerated spleen colonies 11 or repopulated multilineage hematopoiesis 12 in hosts receiving serial transplants. These experiments led to the concept of the hematopoietic stem cell (HSC).TBI has since been used to condition hosts prior to transplantation of donor bone marrow, both therapeutically in humans and as a means of testing murine blood cell subsets for the presence of HSCs. The development of monoclonal antibodies and multiparameter flow cytometry, combined with hematopoietic cell transplantation (HCT), has led to the prospective isolation of murine HSCs, [13][14][15][16] committed lymphoid 17 and myeloerythroid 18 progenitors, and their downstream progeny by cell-surface phenotypes. These powerful techniques have allowed individual components of the immune system to be tested functionally, which has led to the dissection of the hematopoietic hierarchy and the resolution of effector cell function. HCT also provides the means to study the effects of genetic mutations in isolated cell types when introduced into a normal cellular environment.The zebrafish has recently emerged as a unique vertebrate model in which forward genetic screens can uncover novel genes involved in blood cell development and function. 19 Many zebrafish blood mutants identified in these screens are embryonic lethal, and we have recently described embryonic HCT to address issues of cell autonomous mutant gene function. 20 Similar transplantation of whole kidney marrow (WKM) cells into unconditioned adult recipients resulted in the disappearance of donor-derived cells within several weeks of transplantation. This suggests that donorderived cells are either rejected by the host immune system or that engraftment of donor cells requires the creation of niche spa...

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The Zebrafish as a Model Organism to Study Development of the Immune System

Cited by 176 publications

References 240 publications

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>

Animal Models of Tuberculosis: Zebrafish

Effects of lethal irradiation in zebrafish and rescue by hematopoietic cell transplantation

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The Zebrafish as a Model Organism to Study Development of the Immune System

Cited by 176 publications

References 240 publications

Early development of the nervous system of the eutherian &lt;i&gt;Tupaia belangeri&lt;/i&gt;

Early development of the nervous system of the eutherian &lt;i&gt;Tupaia belangeri&lt;/i&gt;

Animal Models of Tuberculosis: Zebrafish

Effects of lethal irradiation in zebrafish and rescue by hematopoietic cell transplantation

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Product

Resources

About

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>

Early development of the nervous system of the eutherian <i>Tupaia belangeri</i>