Activity of brassinosteroids in the dwarf rice lamina inclination bioassay

Fujioka, Shozo; Noguchi, Takahiro; Takatsuto, Suguru; Yoshida, Shigeo

doi:10.1016/s0031-9422(98)00412-9

Cited by 70 publications

(50 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3D), suggesting defective brassinosteroid signaling. In rice, a leaf lamina inclination assay has been successfully used to identify brassinosteroid signaling mutants (Fujioka et al, 1998;Hong et al, 2003). We adopted this method to barley.…”

Section: Phenotypic Identification Of Brassinosteroid Mutants In Barleymentioning

confidence: 99%

Induced Variations in Brassinosteroid Genes Define Barley Height and Sturdiness, and Expand the Green Revolution Genetic Toolkit

et al. 2014

View full text Add to dashboard Cite

Reduced plant height and culm robustness are quantitative characteristics important for assuring cereal crop yield and quality under adverse weather conditions. A very limited number of short-culm mutant alleles were introduced into commercial crop cultivars during the Green Revolution. We identified phenotypic traits, including sturdy culm, specific for deficiencies in brassinosteroid biosynthesis and signaling in semidwarf mutants of barley (Hordeum vulgare). This set of characteristic traits was explored to perform a phenotypic screen of near-isogenic short-culm mutant lines from the brachytic, breviaristatum, dense spike, erectoides, semibrachytic, semidwarf, and slender dwarf mutant groups. In silico mapping of brassinosteroid-related genes in the barley genome in combination with sequencing of barley mutant lines assigned more than 20 historic mutants to three brassinosteroid-biosynthesis genes (BRASSINOSTEROID-6-OXIDASE, CONSTITUTIVE PHOTOMORPHOGENIC DWARF, and DIMINUTO) and one brassinosteroid-signaling gene (BRASSINOSTEROID -INSENSITIVE1 [HvBRI1]). Analyses of F2 and M2 populations, allelic crosses, and modeling of nonsynonymous amino acid exchanges in protein crystal structures gave a further understanding of the control of barley plant architecture and sturdiness by brassinosteroidrelated genes. Alternatives to the widely used but highly temperature-sensitive uzu1.a allele of HvBRI1 represent potential genetic building blocks for breeding strategies with sturdy and climate-tolerant barley cultivars.

show abstract

Section: Phenotypic Identification Of Brassinosteroid Mutants In Barleymentioning

confidence: 99%

Induced Variations in Brassinosteroid Genes Define Barley Height and Sturdiness, and Expand the Green Revolution Genetic Toolkit

et al. 2014

View full text Add to dashboard Cite

show abstract

“…These mutants were obtained from a Nipponbare library of tissue culture-induced mutations [19]. The most wellknown physiological effect of brassinosteroids on growth and development of rice is the increasing of leaf angle (leaf lamina inclination), which has been used as a sensitive bioassay for brassinosteroids [21][22][23][24]. When wildtype seedlings were treated with brassinolide, the leaf angles increased (Figure 1(a)).…”

Section: Gene Expression Analysismentioning

confidence: 99%

Genetic Background Influences Brassinosteroid-Related Mutant Phenotypes in Rice

Sakamoto¹,

Kitano²,

Fujioka³

2013

AJPS

Self Cite

View full text Add to dashboard Cite

In two cases, mutations in the same brassinosteroid-related genes caused different phenotypes in japonica varieties Nipponbare and Taichung 65. The mutant phenotypes were less severe in the Taichung 65 background than in the Nipponbare background. Three newly isolated brassinosteroid-insensitive mutants (d61-1N, d61-11, and d61-12) derived from a Nipponbare mutant library were found to be alleles of d61, which represent defects in the OsBRI1 gene. Although the Nipponbare-derived mutant d61-1N had the same nucleotide substitution as the previously characterized Taichung 65-derived mutant d61-1T, these two mutants showed different phenotypes for plant stature, internode elongation pattern, and seed shape; in each case, d61-1N (in the Nipponbare genetic background) had the more severe mutant phenotype. Similar trends were seen for phenotypes caused by mutants of d2, a brassinosteroid biosynthesis gene. Consistent with these phenotypes, the expression of brassinosteroid-responsive genes was lower in the Nipponbare-derived mutants. These results can be explained by our findings that feed-forward up-regulation of OsBRI1 did not occur in the Nipponbare-derived mutants and that an mPing transposon is inserted into the promoter region of Nipponbare OsBRI1. Based on these results, we conclude that the expression of OsBRI1, especially its feed-forward up-regulation, is misregulated in wild-type Nipponbare and in brassinosteroid-related mutants in a Nipponbare genetic background. Although Nipponbare is a model rice genotype, it can be categorized as an OsBRI1 mutant that has reduced sensitivity to brassinosteroid.

show abstract

“…Detailed method is described in Fujioka et al (1998). Seeds of transgenic plants and the wild type were dehusked, sterilized, and grown on halfstrength Murashige and Skoog medium at 25°C under long-day conditions.…”

Section: Lamina Joint Bending Assaymentioning

confidence: 99%

BRASSINOSTEROID UPREGULATED1, Encoding a Helix-Loop-Helix Protein, Is a Novel Gene Involved in Brassinosteroid Signaling and Controls Bending of the Lamina Joint in Rice

et al. 2009

View full text Add to dashboard Cite

(T.A.) Brassinosteroids (BRs) are involved in many developmental processes and regulate many subsets of downstream genes throughout the plant kingdom. However, little is known about the BR signal transduction and response network in monocots. To identify novel BR-related genes in rice (Oryza sativa), we monitored the transcriptomic response of the brassinosteroid deficient1 (brd1) mutant, with a defective BR biosynthetic gene, to brassinolide treatment. Here, we describe a novel BR-induced rice gene BRASSINOSTEROID UPREGULATED1 (BU1), encoding a helix-loop-helix protein. Rice plants overexpressing BU1 (BU1:OX) showed enhanced bending of the lamina joint, increased grain size, and resistance to brassinazole, an inhibitor of BR biosynthesis. In contrast to BU1:OX, RNAi plants designed to repress both BU1 and its homologs displayed erect leaves. In addition, compared to the wild type, the induction of BU1 by exogenous brassinolide did not require de novo protein synthesis and it was weaker in a BR receptor mutant OsbriI (Oryza sativa brassinosteroid insensitive1, d61) and a rice G protein alpha subunit (RGA1) mutant d1. These results indicate that BU1 protein is a positive regulator of BR response: it controls bending of the lamina joint in rice and it is a novel primary response gene that participates in two BR signaling pathways through OsBRI1 and RGA1. Furthermore, expression analyses showed that BU1 is expressed in several organs including lamina joint, phloem, and epithelial cells in embryos. These results indicate that BU1 may participate in some other unknown processes modulated by BR in rice.Brassinosteroids (BRs) are essential growth regulators, involved in many physiological processes, e.g. cell expansion and division, vascular bundle differentiation, skotomorphogenesis, flowering, senescence, abiotic, and biotic stresses (Szekeres et al

show abstract

Activity of brassinosteroids in the dwarf rice lamina inclination bioassay

Cited by 70 publications

References 23 publications

Induced Variations in Brassinosteroid Genes Define Barley Height and Sturdiness, and Expand the Green Revolution Genetic Toolkit

Induced Variations in Brassinosteroid Genes Define Barley Height and Sturdiness, and Expand the Green Revolution Genetic Toolkit

Genetic Background Influences Brassinosteroid-Related Mutant Phenotypes in Rice

BRASSINOSTEROID UPREGULATED1, Encoding a Helix-Loop-Helix Protein, Is a Novel Gene Involved in Brassinosteroid Signaling and Controls Bending of the Lamina Joint in Rice

Contact Info

Product

Resources

About