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Cited by 11,116 publications
(2,476 citation statements)
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References 21 publications
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“…DNA was purified by extraction with phenol:chloroform:isoamyl alcohol and ethanol precipitation [54]. A. baumannii DNA was obtained from strains isolated from intensive care unit patients and prepared as described previously [55].…”
Section: Genomic Dna and Odn Preparationsmentioning
confidence: 99%
“…DNA was purified by extraction with phenol:chloroform:isoamyl alcohol and ethanol precipitation [54]. A. baumannii DNA was obtained from strains isolated from intensive care unit patients and prepared as described previously [55].…”
Section: Genomic Dna and Odn Preparationsmentioning
confidence: 99%
“…of CT-DNA in buffer gave a ratio of UV absorbance at 260 and 280 nm of ca. 1.9 : 1, indicating that DNA was sufficiently free of protein [56]. The DNA concentration per nucleotide was determined by absorption spectroscopy with the molar absorption coefficient of 6600 dm 3 m À 1 cm À 1 at 260 nm [57].…”
mentioning
confidence: 99%
“…Solutions of DNA in 50 mM NaCl/5 mM Tris-HCl (pH 7.1) gave the ratio of UV absorbance at 260 and 280 nm, A 260 / A 280 of 1.9, indicating that the DNA was sufficiently free of protein [43]. Concentrated stock solutions of DNA were prepared in 50 mM NaCl-5 mM Tris HCl (pH 7.1) and the concentration of DNA in nucleotide phosphate was determined by UV absorbance at 260 nm after 1:100 dilutions.…”
Section: Physical Methodsmentioning
confidence: 99%