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Cited by 71 publications
(39 citation statements)
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References 58 publications
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“…Whereas previous studies have suggested a low content of TSH in teleost fish pituitaries (MacKenzie et al 2009), pituitary expression of the mRNA's representing TSHb and GSUa was readily detectable using a PCR or hybridization-based assay in the pituitary of S. ocellatus. Using cDNA probes to detect steady-state levels of TSHb and GSUa mRNA, we detected a single 877-bp product for TSHb and a single 661-bp product for GSUa in Northern blots of pituitary total RNA.…”
Section: Discussioncontrasting
confidence: 57%
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“…Whereas previous studies have suggested a low content of TSH in teleost fish pituitaries (MacKenzie et al 2009), pituitary expression of the mRNA's representing TSHb and GSUa was readily detectable using a PCR or hybridization-based assay in the pituitary of S. ocellatus. Using cDNA probes to detect steady-state levels of TSHb and GSUa mRNA, we detected a single 877-bp product for TSHb and a single 661-bp product for GSUa in Northern blots of pituitary total RNA.…”
Section: Discussioncontrasting
confidence: 57%
“…A phylogenetic tree of selected vertebrate TSHb and GSUa subunits was constructed by the Mega 4.0.1 software (www.megasoftware.net) using the neighbor-joining method with 1000 replicates. MacKenzie et al (2009) lists the GenBank accession numbers and references of selected vertebrate TSHb and GSUa subunits were used to generate the multiple protein sequence alignments and phylogenetic analysis. The signal peptide was identified using the program SignalP (Center for Biological Sequence Analysis, Technical University of Denmark, www.cbs.dtu.dk).…”
Section: Multiple Protein Sequence Alignment and Phylogenetic Analysismentioning
confidence: 99%
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“…The effects of THs on TSH have been assayed most often by quantification of TSH mRNA expression. A previous study showed that administration of T 3 or T 4 results in substantial inhibition of TSH mRNA expression (MacKenzie et al, 2009). Hence, the increased T 3 levels would feedback to inhibit thyrotropes, as revealed by a down-regulation of TSH beta-subunit gene expression.…”
Section: Discussionmentioning
confidence: 97%