Transcriptomic profiles of the ovaries from piglets neonatally exposed to 4-tert-octylphenol

Knapczyk-Stwora, Katarzyna; Nynca, Anna; Ciereszko, Renata E.; Paukszto, Łukasz; Jastrzebski, J; Czaja, Elzbieta; Witek, Patrycja; Koziorowski, Marek; Słomczyńska, Maria

doi:10.1016/j.theriogenology.2020.04.027

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…The diametric hypothesis addresses the puzzle of why, although 90% of women experience retrograde menstruation (Halme et al, 1984), only 5%-10% of women develop endometriotic lesions: (1) Low Another important hypothesis for the etiology of endometriosis is based on inflammatory activation of developmentally mislocated stem cells, or Müllerian fragments (Russell, 1899;Sasson & Taylor, 2008). By the diametric model, such events are mediated by effects of prenatal testosterone on expression of reproductive developmental genes including HOXA10 (Cermik et al, 2003;He et al, 2018) as well as other genes involved in cell apoptosis, migration, and proliferation (Knapczyk-Stwora et al, 2019;Laganà et al, 2017).…”

Section: Moghadami-tabrizi Et Al (1998) Compared Pcos Patientsmentioning

confidence: 99%

Endometriosis and polycystic ovary syndrome are diametric disorders

Dinsdale

Crespi

2021

Evolutionary Applications

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Evolutionary and comparative approaches can yield novel insights into human adaptation and disease. Endometriosis and polycystic ovary syndrome (PCOS) each affect up to 10% of women and significantly reduce the health, fertility, and quality of life of those affected. PCOS and endometriosis have yet to be considered as related to one another, although both conditions involve alterations to prenatal testosterone levels and atypical functioning of the hypothalamic-pituitary-gonadal (HPG) axis. Here, we propose and evaluate the novel hypothesis that endometriosis and PCOS represent

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Section: Moghadami-tabrizi Et Al (1998) Compared Pcos Patientsmentioning

confidence: 99%

Endometriosis and polycystic ovary syndrome are diametric disorders

Dinsdale

Crespi

2021

Evolutionary Applications

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“… Knapczyk-Stwora et al (2019) found altogether 5,592 RNA sequences to be lncRNAs, among which, 136 (at 53 long non-coding loci) were annotated within relevant databases among 11-day-old piglet ovaries. Meanwhile, the same authors discovered altogether 4,669 RNA sequences to be lncRNAs, among which, 1,236 (at 355 long non-coding loci) were annotated within relevant data-bases in pigs by illumina sequencing ( Knapczyk-Stwora et al, 2020 ). In the current study, we found 92 lncRNAs from porcine GCs by PacBio sequencing, and such number was significantly lower than previously reported.…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of transcriptome complexity in pig granulosa cells by long-read isoform sequencing

Wang

et al. 2022

PeerJ

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Background In intensive and large-scale farms, abnormal estradiol levels in sows can cause reproductive disorders. The high incidence rate of reproductive disturbance will induce the elimination of productive sows in large quantities, and the poor management will bring great losses to the pig farms. The change in estradiol level has an important effect on follicular development and estrus of sows. To solve this practical problem and improve the productive capacity of sows, it is significant to further clarify the regulatory mechanism of estradiol synthesis in porcine granulosa cells (GCs). The most important function of granulosa cells is to synthesize estradiol. Thus, the studies about the complex transcriptome in porcine GCs are significant. As for precursor-messenger RNAs (pre-mRNAs), their post-transcriptional modification, such as alternative polyadenylation (APA) and alternative splicing (AS), together with long non-coding RNAs (lncRNAs), may regulate the functions of granulosa cells. However, the above modification events and their function are unclear within pig granulosa cells. Methods Combined PacBio long-read isoform sequencing (Iso-Seq) was conducted in this work for generating porcine granulosa cells’ transcriptomic data. We discovered new transcripts and possible gene loci via comparison against reference genome. Later, combined Iso-Seq data were adopted to uncover those post-transcriptional modifications such as APA or AS, together with lncRNA within porcine granulosa cells. For confirming that the Iso-Seq data were reliable, we chose four AS genes and analyzed them through RT-PCR. Results The present article illustrated that pig GCs had a complex transcriptome, which gave rise to 8,793 APA, 3,465 AS events, 703 candidate new gene loci, as well as 92 lncRNAs. The results of this study revealed the complex transcriptome in pig GCs. It provided a basis for the interpretation of the molecular mechanism in GCs.

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“…We demonstrated that neonatal exposure to androgen and estrogen agonists or antagonists, such as testosterone propionate (TP; a synthetic androgen), flutamide (FLU; a nonsteroidal antiandrogen), 4-tert-octylphenol (OP; compound with estrogenic activity), ICI 182,780 (ICI; a pure antiestrogen), and methoxychlor (MXC; compound with estrogenic, antiestrogenic and antiandrogenic properties), influenced the number of primordial and primary follicles in piglets (Knapczyk-Stwora et al, 2018). Moreover, using a RNA sequencing approach, we showed that neonatal exposure of piglets to FLU and OP altered the expression of genes involved in ovarian cell proliferation, apoptosis, and steroidogenesis (Knapczyk-Stwora et al, 2019;2020a). Thus, both androgens and estrogens are important for the proper follicle assembly and activation in neonatal porcine ovaries.…”

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confidence: 94%

Effects of Sex Steroid Receptor Agonists and Antagonists on the Expression of the FOXL2 Transcription Factor and its Target Genes AMH and CYP19A1 in the Neonatal Porcine Ovary

Witek

Marek

Grzesiak

et al. 2022

Annals of Animal Science

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Recently, we have demonstrated that neonatal exposure to androgen and estrogen agonists or antagonists influenced the number of ovarian follicles in piglets. Since the FOXL2 transcription factor is required for proper ovarian follicle formation and activation, the objective of the study was to examine effects of exposure of the neonatal porcine ovary to testosterone propionate (TP; an androgen), flutamide (FLU; an antiandrogen), 4-tert-octylphenol (OP; compound with estrogenic activity), ICI 182,780 (ICI; an antiestrogen), and methoxychlor (MXC; compound with estrogenic, antiestrogenic and antiandrogenic properties) on FOXL2 expression and expression of its target genes, AMH and CYP19A1. Piglets were injected subcutaneously with TP, FLU, OP, ICI, MXC, or corn oil (control) between postnatal days 1 and 10 (n = 4/each group). Ovaries were excised from the 11-day-old piglets and the expression of FOXL2, AMH and CYP19A1 were examined using immunohistochemistry and/or real-time PCR and Western blot. FOXL2 was localized in stroma cells surrounding egg nests and in granulosa cells. TP, OP and MXC increased both FOXL2 and AMH mRNAs, while FLU and ICI decreased CYP19A1 mRNA. The increased FOXL2 protein abundance was found in all examined groups. In addition, TP, OP, ICI and MXC increased AMH protein abundance, while TP, FLU and OP decreased CYP19A1 protein abundance. In conclusion, neonatal exposure to sex steroid receptor agonists and antagonists increased FOXL2 expression at mRNA and/or protein levels and affected FOXL2 target genes in the ovaries of 11-day-old piglets. Therefore, it seems that impaired ovarian folliculogenesis induced by altered steroid milieu during the neonatal development period in pigs may, at least in part, involve FOXL2.

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Transcriptomic profiles of the ovaries from piglets neonatally exposed to 4-tert-octylphenol

Cited by 7 publications

References 44 publications

Endometriosis and polycystic ovary syndrome are diametric disorders

Endometriosis and polycystic ovary syndrome are diametric disorders

Integrative analysis of transcriptome complexity in pig granulosa cells by long-read isoform sequencing

Effects of Sex Steroid Receptor Agonists and Antagonists on the Expression of the FOXL2 Transcription Factor and its Target Genes AMH and CYP19A1 in the Neonatal Porcine Ovary

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