2015
DOI: 10.1016/j.tcb.2015.09.010
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Abstract: Although it is widely appreciated that cells migrate in a variety of diverse environments in vivo, we are only now beginning to use experimental workflows that yield images with sufficient spatiotemporal resolution to study the molecular processes governing cell migration in 3D environments. Since cell migration is a dynamic process, it is usually studied via microscopy, but 3D movies of 3D processes are difficult to interpret by visual inspection. In this review, we discuss the technologies required to study … Show more

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Cited by 55 publications
(51 citation statements)
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“…Visualizing 3D data can be cumbersome and identifying the temporal and spatial properties of subcellular structures in 3D is difficult if not impossible via manual identification and tracking (Driscoll and Danuser, 2015); this fact is exacerbated by the quantity of data generated by meSPIM. Here, we demonstrate that meSPIM produces images of sufficient quality to identify and track blebs, which are relatively small and short-lived compared with other protrusion types, in an automated and high-throughput fashion.…”
Section: Resultsmentioning
confidence: 99%
“…Visualizing 3D data can be cumbersome and identifying the temporal and spatial properties of subcellular structures in 3D is difficult if not impossible via manual identification and tracking (Driscoll and Danuser, 2015); this fact is exacerbated by the quantity of data generated by meSPIM. Here, we demonstrate that meSPIM produces images of sufficient quality to identify and track blebs, which are relatively small and short-lived compared with other protrusion types, in an automated and high-throughput fashion.…”
Section: Resultsmentioning
confidence: 99%
“…(Lammermann and Sixt, 2009),(Diz-Munoz et al, 2016)), as well as for other cellular events where blebs are found (e.g., (Norman et al, 2010),(Laster and Mackenzie, 1996). It would also be interesting to determine if the distribution or the dynamics of such invaginations are altered upon the transition between lamellipodia and bleb-driven migration in cells that can switch between the two modes of protrusion formation (Diz-Munoz et al, 2016; Driscoll and Danuser, 2015; Friedl and Wolf, 2010; Welch, 2015). …”
Section: Discussionmentioning
confidence: 99%
“…It is still not clear how many different mechanisms can be used by cells to move. Automated, unbiased imaging approaches could more rapidly establish how many distinct mechanisms cells can use to migrate [30,64]. The function of each mode of migration is also unclear.…”
Section: Discussionmentioning
confidence: 99%
“…More recently, studying changes in tumor cell morphology led to the discovery of the mesenchymal (elongated) and amoeboid (rounded) modes of 3D cell migration [28,29]. It is now clear that many cell types can use distinct mechanisms to move through diverse 3D environments [30]. These modes of 3D cell migration are most easily classified by their relative cell-matrix adhesion and actomyosin contractility (Figure 1).…”
Section: The Plasticity Of 3d Cell Movementmentioning
confidence: 99%