Ratiometric fluorescence detection of hydroxyl radical using cyanine-based binary nanoGUMBOS

Cong, Mingyan; Siraj, Noureen; Bhattarai, Nimisha; Kolic, Paulina E.; McCarter, Kevin S.; Chhotaray, Pratap K.; Warner, Isiah M.

doi:10.1016/j.snb.2017.10.101

Cited by 14 publications

(11 citation statements)

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“…In recent years, a group of uniform materials based on organic salts (GUMBOS) has been developed in our laboratory for several biomedical applications. 2,[25][26][27] Although similar to ionic liquids in terms of counter-ions used, GUMBOS are solidphase analogs with a dened melting point range of 25 C to 250 C. Such organic salts are found to have broadly tunable properties such as toxicity, hydrophobicity, melting point, and optical behaviors. 2,25,26,28,29 Thus, GUMBOS can be designed for specic tasks through simple counter-ion variations.…”

Section: Introductionmentioning

confidence: 99%

Mitochondria targeting IR780-based nanoGUMBOS for enhanced selective toxicity towards cancer cells

et al. 2018

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Section: Introductionmentioning

confidence: 99%

Mitochondria targeting IR780-based nanoGUMBOS for enhanced selective toxicity towards cancer cells

et al. 2018

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“…TC-GUMBOS aggregates were prepared using a simple reprecipitation method. 25 Briefly, 50 μL of 0.5 mM ethanolic solution of each TC-GUMBOS was dropped into 5 mL of 10 mM sodium phosphate buffer (pH = 7.4) under a sonication bath for 3 min. The resulting aqueous solution was left to rest for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Protein Discrimination Using a Fluorescence-Based Sensor Array of Thiacarbocyanine-GUMBOS

et al. 2020

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Sensitive and selective detection of proteins from complex samples has gained substantial interest within the scientific community. Early and precise detection of key proteins plays an important role in potential clinical diagnosis, treatment of different diseases, and proteomic research. In the study reported here, six different compounds belonging to a group of uniform materials based on organic salts (GUMBOS) have been synthesized using three thiacarbocyanine (TC) dyes and employed as fluorescent sensors. Fluorescence properties of micro- and nanoaggregates of these TC-based GUMBOS formed in phosphate buffer solutions are studied in the absence and presence of seven proteins. Fluorescence response patterns of these TC-based GUMBOS were analyzed by linear discriminant analysis (LDA). The constructed LDA model allowed discrimination of these seven proteins at various concentrations with 100% accuracy. The sensing and discrimination abilities of these TC-based GUMBOS were further evaluated in mixtures of two major proteins, i.e., human serum albumin and hemoglobin. Fluorescence response patterns of these mixtures were analyzed by LDA. This model allowed discrimination of various mixtures with 100% accuracy. Moreover, spiked urine samples were prepared and the responses of these sensors were collected and analyzed by LDA. Remarkably, discrimination of these seven proteins was also achieved with 100% accuracy.

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“…While fluorescence imaging offers many advantages as a detection method, there are several limitations when developing successful probes for real-time detection. These include instrument sensitivity, source light fluctuations, temperature, concentration variations, and environmental factors [ 237 ]. Additionally, as hydroxyl radicals (•OH) are present at low concentrations and have a short lifetime, it can be extremely difficult to properly detect their presence in vivo.…”

Section: Hydroxyl Radical (•Oh) Detectionmentioning

confidence: 99%

“…A second solution to the common challenges associated with fluorescence detection for •OH was proposed by Cong et al [ 237 ], who employed a ratiometric fluorescence detection method. This study involved the development of cyanide-based nano-Group of Uniform Materials Based on Organic Salts (nanoGUMBOS) to be used as a fluorescent probe for •OH detection.…”

Section: Hydroxyl Radical (•Oh) Detectionmentioning

confidence: 99%

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

et al. 2021

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Reactive oxygen species (ROS) have been found in plants, mammals, and natural environmental processes. The presence of ROS in mammals has been linked to the development of severe diseases, such as diabetes, cancer, tumors, and several neurodegenerative conditions. The most common ROS involved in human health are superoxide (O2•−), hydrogen peroxide (H2O2), and hydroxyl radicals (•OH). Organic and inorganic molecules have been integrated with various methods to detect and monitor ROS for understanding the effect of their presence and concentration on diseases caused by oxidative stress. Among several techniques, fluorescence and electrochemical methods have been recently developed and employed for the detection of ROS. This literature review intends to critically discuss the development of these techniques to date, as well as their application for in vitro and in vivo ROS detection regarding free-radical-related diseases. Moreover, important insights into and further steps for using fluorescence and electrochemical methods in the detection of ROS are presented.

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Ratiometric fluorescence detection of hydroxyl radical using cyanine-based binary nanoGUMBOS

Cited by 14 publications

References 39 publications

Mitochondria targeting IR780-based nanoGUMBOS for enhanced selective toxicity towards cancer cells

Mitochondria targeting IR780-based nanoGUMBOS for enhanced selective toxicity towards cancer cells

Protein Discrimination Using a Fluorescence-Based Sensor Array of Thiacarbocyanine-GUMBOS

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

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