Quantitative uptake of colloidal particles by cell cultures

Quantitative Particle Uptake by Cells as Analyzed by Different Methods

“…To describe particle uptake by cells, most importantly all the details about the experimental set‐up need to be provided . Concerning the particles, this involves details about the metrics with which particle concentrations are quantified . This can be in the form of molar particle concentrations, mass concentrations, molar concentrations of elements, total particle volume, total particle surface, total particle absorption, total particle fluorescence, etc .…”

“…When referring to cells, this could involve the percentage of cells which have internalized particles, which is, for example, frequently used in flow cytometry studies, or the percentage of cells which have a certain number of particles incorporated, which is often used in case the number of discrete particles in a cell can be directly counted . When referring to particles, this could be the percentage of particles which have been internalized, which, for example, is done when the amount of particles remaining in the cell medium and the amount of particles inside cells are quantified with elemental analysis . Second, also the t ‐axis in Figure , the second quantifier, addressing the question “how fast”, needs to be defined.…”

Monitoring the death of single BaF3 cells under plasmonic photothermal heating induced by ultrasmall gold nanorods

“…It is well established in the literature that most nanoparticles, when endocytosed by a cell, eventually primarily accumulate in the lysosomes. 15,[44][45][46][47][48][49][50][51] This is because the early endosomes, which contain the endocytosed material, are acidified and fuse with pre-lysosomal vesicles, before they turn into late endosomes and consequentially lysosomes. 52 A break-out of the nanoparticles from the endosomes on this pathway is unlikely unless endosomal escape strategies are employed.…”

Analyse quantitativer Partikelaufnahme von Zellen über verschiedene Messmethoden

“…Um Partikelaufnahme von Zellen zu beschreiben, ist von höchster Wichtigkeit, dass alle Details des experimentellen Aufbaus zur Verfügung stehen . In Bezug auf die Partikel sind damit die Details der Einheitensysteme gemeint, mit denen Partikelkonzentrationen quantifiziert werden . Dies kann in der Form von molaren Partikelkonzentrationen, Gewichtskonzentrationen, molaren Konzentrationen der Elemente, dem gesamten Partikelvolumen, der gesamten Partikeloberfläche, der gesamten Partikelabsorption, der gesamten Partikelfluoreszenz usw.…”