Fabrication of cell patches using biodegradable scaffolds with a hexagonal array of interconnected pores (SHAIPs)

Zhang, Yu Shrike; Yao, Junjie; Wang, Lihong V.; Xia, Younan

doi:10.1016/j.polymer.2013.06.019

Cited by 11 publications

(5 citation statements)

References 53 publications

(59 reference statements)

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“…[124] Similar to the fabrication process of an inverse opal scaffold, the PLGA scaffold with a hexagonal array of interconnected pores (SHAIP) was produced by templating against gelatin microspheres assembled in a single layer lattice (Figure 21A–D). As a model construct, the homogeneous skeletal muscle patches were created by seeding myoblasts into the SHAIPs.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Inverse Opal Scaffolds and Their Biomedical Applications

2017

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Three-dimensional porous scaffolds play a pivotal role in tissue engineering and regenerative medicine by functioning as biomimetic substrates to manipulate cellular behaviors. While many techniques have been developed to fabricate porous scaffolds, most of them rely on stochastic processes that typically result in scaffolds with pores uncontrolled in terms of size, structure, and interconnectivity, greatly limiting their use in tissue regeneration. Inverse opal scaffolds, in contrast, possess uniform pores inheriting from the template comprised of a closely packed lattice of monodispersed microspheres. The key parameters of such scaffolds, including architecture, pore structure, porosity, and interconnectivity, can all be made uniform across the same sample and among different samples. In conjunction with a tight control over pore sizes, inverse opal scaffolds have found widespread use in biomedical applications. In this review, we provide a detailed discussion on this new class of advanced materials. After a brief introduction to their history and fabrication, we highlight the unique advantages of inverse opal scaffolds over their non-uniform counterparts. We then showcase their broad applications in tissue engineering and regenerative medicine, followed by a summary and perspective on future directions.

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Section: Biomedical Applicationsmentioning

confidence: 99%

Inverse Opal Scaffolds and Their Biomedical Applications

2017

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“…It is clear that the AJS platform could effectively produce fine nanoscale fibers (268.46±44.21 nm) from pure PA6 (S1 scaffold) solution (figures 1(A) and (A * )) with a smooth surface pattern (inset of figure 1(A * )), but without interconnectivity between each fiber. A tissue scaffold matrix with interconnected pores can function as physical supports for cells to adhere and enlarge over a relatively large volume [20]. The reason for the formation of non-connected network fibers from pure PA6 is attributed to the low spinning rate of PA6 solution (1.3 ml min −1 ) through AJS process.…”

Section: Resultsmentioning

confidence: 99%

Biocompatibility properties of polyamide 6/PCL blends composite textile scaffold using EA.hy926 human endothelial cells

et al. 2017

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Enhancing the cytocompatibility profiles, including cell attachment, growth and viability, of designed synthetic scaffolds, has a pivotal role in tissue engineering applications. Polymer blending is one of the most effective methods for providing new desirable biomaterials for tissue scaffolds. This article reports a novel polyamide 6/poly(ε-caprolactone) (PA6/PCL) blends solution which was fabricated to create composite fibrous tissue scaffolds by varying the concentration ratios of PA6 and PCL. Highly porous blends of fibrous scaffold were fabricated and their suitability as cell-support for EA.hy926 human endothelial cells was studied. Our results demonstrated that the unique nanoscale morphological properties and tune porosity of the blends scaffold were controlled. We found that these properties are mainly dependent on the PA6/PCL blending viscosity value, and the viscosity of the blending solution has an intense effect on the properties of the blends scaffold. The influence of the scaffolds extraction fluids and the scaffold direct contact of both the metabolic viability and the DNA integrity of EA.hy926 endothelial cells, as well as the cell/scaffold interaction analysis by scanning electron microscope, after different co-culturing intervals, demonstrated that PA6/PCL blend scaffolds showed different behaviors. Blend scaffolds of PA6/PCL of 90:10 ratio proved to be excellent endothelial cell carriers, which provided a good cell morphology, DNA integrity and viability, induced DNA synthesis/replication, and enhanced cell proliferation, attachment, and invasion. These results indicate that blends of PA6/PCL composite fibers are a promising 3D substitute for the next generation of synthetic tissue scaffolds that could soon find clinical applications.

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“…30,43 In order to arrange microparticles into crystalline configurations, fluidic forces and solvent evaporation can be controlled and coupled with agitation to increase the time and opportunity for particle interactions. 44,45 The methods described in this review do not focus on those induced using only magnets (albeit effective), 46 but rather focus on methods that can be applied to a broader set of particle compositions and particle/solvent combinations.…”

Section: Microparticle Assembly For Particle-based Crystal Fabricationmentioning

confidence: 99%

“…Under these modified conditions, periodic close-packed crystals formed from gentle tapping on the sides of the container during the solvent evaporation process. 45,60 2.2 Granular-particle assembly for particle-based crystal fabrication Packing granular particles has been widely researched across the literature to study the packing density of particles and powders and their subsequent phase behavior. For example, particles of a variety of material compositions, including glass and metal, have been transformed from a granular gaseous phase to a crystalline one by tuning the vibration applied to the holding container in clever ways.…”

Section: Microparticle Assembly For Particle-based Crystal Fabricationmentioning

confidence: 99%

Scaling up self-assembly: bottom-up approaches to macroscopic particle organization

et al. 2015

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This review presents an overview of recent work in the field of non-Brownian particle self-assembly. Compared to nanoparticles that naturally self-assemble due to Brownian motion, larger, non-Brownian particles (d > 6 μm) are less prone to autonomously organize into crystalline arrays. The tendency for particle systems to experience immobilization and kinetic arrest grows with particle radius. In order to overcome this kinetic limitation, some type of external driver must be applied to act as an artificial "thermalizing force" upon non-Brownian particles, inducing particle motion and subsequent crystallization. Many groups have explored the use of various agitation methods to overcome the natural barriers preventing self-assembly to which non-Brownian particles are susceptible. The ability to create materials from a bottom-up approach with these characteristics would allow for precise control over their pore structure (size and distribution) and surface properties (topography, functionalization and area), resulting in improved regulation of key characteristics such as mechanical strength, diffusive properties, and possibly even photonic properties. This review will highlight these approaches, as well as discuss the potential impact of bottom-up macroscale particle assembly. The applications of such technology range from customizable and autonomously self-assembled niche microenvironments for drug delivery and tissue engineering to new acoustic dampening, battery, and filtration materials, among others. Additionally, crystals made from non-Brownian particles resemble naturally derived materials such as opals, zeolites, and biological tissue (i.e. bone, cartilage and lung), due to their high surface area, pore distribution, and tunable (multilevel) hierarchy.

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Fabrication of cell patches using biodegradable scaffolds with a hexagonal array of interconnected pores (SHAIPs)

Cited by 11 publications

References 53 publications

Inverse Opal Scaffolds and Their Biomedical Applications

Inverse Opal Scaffolds and Their Biomedical Applications

Biocompatibility properties of polyamide 6/PCL blends composite textile scaffold using EA.hy926 human endothelial cells

Scaling up self-assembly: bottom-up approaches to macroscopic particle organization

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