The wheat SnRK1α family and its contribution to Fusarium toxin tolerance

Perochon, Alexandre; Váry, Zsolt; Malla, Keshav B.; Halford, Nigel G.; Paul, M. J.; Doohan, Fiona M.

doi:10.1016/j.plantsci.2019.110217

Cited by 27 publications

(15 citation statements)

References 84 publications

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“…It has been proved that many a transcription factor could directly or indirectly regulate plants immune response [26,[44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62]. Here, the current study got the similar result (Fig.…”

Section: Defense-related Transcription Factorssupporting

confidence: 71%

Transcriptome analysis reveals underlying immune response mechanism of fungal (Penicillium oxalicum) disease in Gastrodia elata Bl. f. glauca S. chow (Orchidaceae)

et al. 2020

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Background Gastrodia elata Bl. f. glauca S. Chow is a medicinal plant. G. elata f. glauca is unavoidably infected by pathogens in their growth process. In previous work, we have successfully isolated and identified Penicillium oxalicum from fungal diseased tubers of G. elata f. glauca. As a widespread epidemic, this fungal disease seriously affected the yield and quality of G. elata f. glauca. We speculate that the healthy G. elata F. glauca might carry resistance genes, which can resist against fungal disease. In this study, healthy and fungal diseased mature tubers of G. elata f. glauca from Changbai Mountain area were used as experimental materials to help us find potential resistance genes against the fungal disease. Results A total of 7540 differentially expressed Unigenes (DEGs) were identified (FDR < 0.01, log2FC > 2). The current study screened 10 potential resistance genes. They were attached to transcription factors (TFs) in plant hormone signal transduction pathway and plant pathogen interaction pathway, including WRKY22, GH3, TIFY/JAZ, ERF1, WRKY33, TGA. In addition, four of these genes were closely related to jasmonic acid signaling pathway. Conclusions The immune response mechanism of fungal disease in G. elata f. glauca is a complex biological process, involving plant hormones such as ethylene, jasmonic acid, salicylic acid and disease-resistant transcription factors such as WRKY, TGA.

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Section: Defense-related Transcription Factorssupporting

confidence: 71%

Transcriptome analysis reveals underlying immune response mechanism of fungal (Penicillium oxalicum) disease in Gastrodia elata Bl. f. glauca S. chow (Orchidaceae)

et al. 2020

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“…The wheat genome has three TaSnRK1α homoelogues (A, B, and D) that share the same AA sequence and have only 12-14 nucleotide differences in the coding regions 50 . Because all the five TnSnRK1α clones identified in the original yeast two-hybrid library screen were TaSnRK1α-A, we used TnSnRK1α-A for all the experiments related to TaSnRK1α unless otherwise stated.…”

Section: Resultsmentioning

confidence: 99%

“…In rice, the expression of OsSnRK1α was reported to be associated with disease resistance against M. oryzae, Cochliobolus miyabeanus, and Rhizoctonia solani 64,65 but its exact role in disease resistance has not yet been characterized. In wheat, the kinase activity of TaSnRK1α was increased in the presence of DON produced by F. graminearum 50 . In this study, we showed that overexpression of TaSnRK1α in wheat increased resistance against F. graminearum but transgenic plants expressing the SnRK1 silencing construct were more susceptible.…”

Section: Discussionmentioning

confidence: 99%

An orphan protein of Fusarium graminearum modulates host immunity by mediating proteasomal degradation of TaSnRK1α

et al. 2020

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Fusarium graminearum is a causal agent of Fusarium head blight (FHB) and a deoxynivalenol (DON) producer. In this study, OSP24 is identified as an important virulence factor in systematic characterization of the 50 orphan secreted protein (OSP) genes of F. graminearum. Although dispensable for growth and initial penetration, OSP24 is important for infectious growth in wheat rachis tissues. OSP24 is specifically expressed during pathogenesis and its transient expression suppresses BAX-or INF1-induced cell death. Osp24 is translocated into plant cells and two of its 8 cysteine-residues are required for its function. Wheat SNF1-related kinase TaSnRK1α is identified as an Osp24-interacting protein and shows to be important for FHB resistance in TaSnRK1α-overexpressing or silencing transgenic plants. Osp24 accelerates the degradation of TaSnRK1α by facilitating its association with the ubiquitin-26S proteasome. Interestingly, TaSnRK1α also interacts with TaFROG, an orphan wheat protein induced by DON. TaFROG competes against Osp24 for binding with the same region of TaSnRKα and protects it from degradation. Overexpression of TaFROG stabilizes TaSnRK1α and increases FHB resistance. Taken together, Osp24 functions as a cytoplasmic effector by competing against TaFROG for binding with TaSnRK1α, demonstrating the counteracting roles of orphan proteins of both host and fungal pathogens during their interactions.

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“…This suggests that engineering the trichothecene target sites could help the plants to continue the production of proteins and confer better resistance against the pathogen. Another study demonstrated the relation between DON synthesis and TaFROG ( Triticum aestivum Fusarium resistance orphan gene) and one of its interacting partner, a sucrose non-fermenting-1 (SNF1)-related protein kinase 1 catalytic subunit α (SnRK1α) [154]. TaFROG improved wheat resistance to DON, but the role of SnRK1α genes in disease resistance is not well known.…”

Section: Effect Of Mycotoxins On Plant Secondary Metabolite Producmentioning

confidence: 99%

Fusarium-Produced Mycotoxins in Plant-Pathogen Interactions

Perincherry

Lalak-Kańczugowska

Stępień

2019

Toxins

142

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Pathogens belonging to the Fusarium genus are causal agents of the most significant crop diseases worldwide. Virtually all Fusarium species synthesize toxic secondary metabolites, known as mycotoxins; however, the roles of mycotoxins are not yet fully understood. To understand how a fungal partner alters its lifestyle to assimilate with the plant host remains a challenge. The review presented the mechanisms of mycotoxin biosynthesis in the Fusarium genus under various environmental conditions, such as pH, temperature, moisture content, and nitrogen source. It also concentrated on plant metabolic pathways and cytogenetic changes that are influenced as a consequence of mycotoxin confrontations. Moreover, we looked through special secondary metabolite production and mycotoxins specific for some significant fungal pathogens-plant host models. Plant strategies of avoiding the Fusarium mycotoxins were also discussed. Finally, we outlined the studies on the potential of plant secondary metabolites in defense reaction to Fusarium infection.

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The wheat SnRK1α family and its contribution to Fusarium toxin tolerance

Cited by 27 publications

References 84 publications

Transcriptome analysis reveals underlying immune response mechanism of fungal (Penicillium oxalicum) disease in Gastrodia elata Bl. f. glauca S. chow (Orchidaceae)

Transcriptome analysis reveals underlying immune response mechanism of fungal (Penicillium oxalicum) disease in Gastrodia elata Bl. f. glauca S. chow (Orchidaceae)

An orphan protein of Fusarium graminearum modulates host immunity by mediating proteasomal degradation of TaSnRK1α

Fusarium-Produced Mycotoxins in Plant-Pathogen Interactions

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