Transcriptional control of the cell cycle

Berckmans, Barbara; Veylder, Lieven De

doi:10.1016/j.pbi.2009.07.005

Cited by 117 publications

(74 citation statements)

References 57 publications

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“…The enhanced expression of TFs involved in transcription, translation, and cell elongation at the site of infection is consistent with the observed endoreduplication. However, we did not observe statistically significant differences in expression for known TFs that mediate endoreduplication (28,29). This could be because most of these TFs were identified in developmental studies of specialized cells with enhanced ploidy (e.g., trichomes) or because their activity is not regulated at the transcript level.…”

Section: Genes Involved In Calcium Signaling and Redox Regulation Larmentioning

confidence: 60%

Laser microdissection of Arabidopsis cells at the powdery mildew infection site reveals site-specific processes and regulators

Chandran

Inada²,

Hather³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

181

135

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To elucidate host processes and components required for the sustained growth and reproduction of the obligate biotrophic fungus Golovinomyces orontii on Arabidopsis thaliana , laser microdissection was used to isolate cells at the site of infection at 5 days postinfection for downstream global Arabidopsis expression profiling. Site-specific profiling increased sensitivity dramatically, allowing us to identify specific host processes, process components, and their putative regulators hidden in previous whole-leaf global expression analyses. For example, 67 transcription factors exhibited altered expression at the powdery mildew (PM) infection site, with subsets of these playing known or inferred roles in photosynthesis, cold/dehydration responses, defense, auxin signaling, and the cell cycle. Using integrated informatics analyses, we constructed putative regulatory networks for a subset of these processes and provided strong support for host cell cycle modulation at the PM infection site. Further experimentation revealed induced host endoreduplication occurred exclusively at the infection site and led us to identify MYB3R4 as a transcriptional regulator of this process. Induced endoreduplication was abrogated in myb3r4 mutants, and G. orontii growth and reproduction were reduced. This suggests that, by increasing gene copy number, localized endoreduplication serves as a mechanism to meet the enhanced metabolic demands imposed by the fungus, which acquires all its nutrients from the plant host.

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Section: Genes Involved In Calcium Signaling and Redox Regulation Larmentioning

confidence: 60%

Laser microdissection of Arabidopsis cells at the powdery mildew infection site reveals site-specific processes and regulators

Chandran

Inada²,

Hather³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

181

135

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“…Whereas a robust molecular framework is emerging for the control of G1/S-phase transition in plants (Zhao et al, 2012), less is known about the regulatory modules promoting G2/Mphase transition (Berckmans and De Veylder, 2009). Our study provides insights into how cell type-specific mechanisms drive G2/M-phase transition, and we propose that the DUO1-DAZ1/ DAZ2 regulatory module controls mitotic division in the male germline ( Figure 9).…”

Section: A Duo1-daz1/daz2 Regulatory Module Controls Germ Cell G2/m-pmentioning

confidence: 82%

An EAR-Dependent Regulatory Module Promotes Male Germ Cell Division and Sperm Fertility in Arabidopsis

et al. 2014

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The production of the sperm cells in angiosperms requires coordination of cell division and cell differentiation. In Arabidopsis thaliana, the germline-specific MYB protein DUO1 integrates these processes, but the regulatory hierarchy in which DUO1 functions is unknown. Here, we identify an essential role for two germline-specific DUO1 target genes, DAZ1 and DAZ2, which encode EAR motif-containing C 2 H 2 -type zinc finger proteins. We show that DAZ1/DAZ2 are required for germ cell division and for the proper accumulation of mitotic cyclins. Importantly, DAZ1/DAZ2 are sufficient to promote G2-to M-phase transition and germ cell division in the absence of DUO1. DAZ1/DAZ2 are also required for DUO1-dependent cell differentiation and are essential for gamete fusion at fertilization. We demonstrate that the two EAR motifs in DAZ1/DAZ2 mediate their function in the male germline and are required for transcriptional repression and for physical interaction with the corepressor TOPLESS. Our findings uncover an essential module in a regulatory hierarchy that drives mitotic transition in male germ cells and implicates gene repression pathways in sperm cell formation and fertility.

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“…Additionally, the proposed regulatory networks suggest a central role of the E2F/RBR regulatory pathway, particularly of E2Fc, in the regulation of cenH3 and KNL2 expression. Transcription of cenH3 and KNL2 in nondividing tissues might be repressed by (1) E2Fc, which generally inhibits E2Fc-responsive genes (del Pozo et al, 2002(del Pozo et al, , 2006 and apparently occupies a central role in this regulatory network; (2) an interplay of typical and atypical E2Fs (Berckmans and De Veylder, 2009); and/or (3) E2F/RBR, recruiting enzymes with chromatin silencing/ inactivating features to E2F-responsive promoters (Luo et al, 1998;van den Heuvel and Dyson, 2008), such as a DNA methyltransferase, which might methylate KNL2 and cenH3 promoters in nondividing tissues. The latter mechanism would explain the increased expression of cenH3 (3.3-fold) and KNL2 (3.5-fold) in met1-3 mutants with a reduced level of CG methylation (Mathieu et al, 2007).…”

Section: Cenh3 Assembly Is Regulated At the Transcriptional Levelmentioning

confidence: 99%

Arabidopsis KINETOCHORE NULL2 Is an Upstream Component for Centromeric Histone H3 Variant cenH3 Deposition at Centromeres

et al. 2013

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The centromeric histone H3 variant cenH3 is an essential centromeric protein required for assembly, maintenance, and proper function of kinetochores during mitosis and meiosis. We identified a KINETOCHORE NULL2 (KNL2) homolog in Arabidopsis thaliana and uncovered features of its role in cenH3 loading at centromeres. We show that Arabidopsis KNL2 colocalizes with cenH3 and is associated with centromeres during all stages of the mitotic cell cycle, except from metaphase to mid-anaphase. KNL2 is regulated by the proteasome degradation pathway. The KNL2 promoter is mainly active in meristematic tissues, similar to the cenH3 promoter. A knockout mutant for KNL2 shows a reduced level of cenH3 expression and reduced amount of cenH3 protein at chromocenters of meristematic nuclei, anaphase bridges during mitosis, micronuclei in pollen tetrads, and 30% seed abortion. Moreover, knl2 mutant plants display reduced expression of suppressor of variegation 3-9 homologs2, 4, and 9 and reduced DNA methylation, suggesting an impact of KNL2 on the epigenetic environment for centromere maintenance.

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Transcriptional control of the cell cycle

Cited by 117 publications

References 57 publications

Laser microdissection of Arabidopsis cells at the powdery mildew infection site reveals site-specific processes and regulators

Laser microdissection of Arabidopsis cells at the powdery mildew infection site reveals site-specific processes and regulators

An EAR-Dependent Regulatory Module Promotes Male Germ Cell Division and Sperm Fertility in Arabidopsis

Arabidopsis KINETOCHORE NULL2 Is an Upstream Component for Centromeric Histone H3 Variant cenH3 Deposition at Centromeres

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