MUC-1 aptamer conjugated InP/ZnS quantum dots/nanohydrogel fluorescent composite for mitochondria-mediated apoptosis in MCF-7 cells

Ranjbar-Navazi, Zahra; Fathi, Marziyeh; Abdolahinia, Elaheh Dalir; Omidi, Yadollah; Davaran, Soodabeh

doi:10.1016/j.msec.2020.111469

Cited by 31 publications

(15 citation statements)

References 56 publications

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“…Moreover, if inhibiting LDH can, in some cases, strengthen the respiratory capacity of non-dividing cancer cells, this could foster stem cell phenotype and lead to propagation of a metabolic rewiring, which could later lead to invasive metastasis [ 11 , 36 ], creating counter indication of LDH inhibitor drugs. [ 11 ] The latter question is currently being addressed by research investigating combination use of LDH inhibitors with mitochondrial OXPHOS or the Krebs cycle-inhibiting drugs [ 37 , 38 ] either together or adjunct to traditional chemotherapy drugs such as paclitaxel [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Triple Isozyme Lactic Acid Dehydrogenase Inhibition in Fully Viable MDA-MB-231 Cells Induces Cytostatic Effects That Are Not Reversed by Exogenous Lactic Acid

et al. 2021

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A number of aggressive human malignant tumors are characterized by an intensified glycolytic rate, over-expression of lactic acid dehydrogenase A (LDHA), and subsequent lactate accumulation, all of which contribute toward an acidic peri-cellular immunosuppressive tumor microenvironment (TME). While recent focus has been directed at how to inhibit LDHA, it is now becoming clear that multiple isozymes of LDH must be simultaneously inhibited in order to fully suppress lactic acid and halt glycolysis. In this work we explore the biochemical and genomic consequences of an applied triple LDH isozyme inhibitor (A, B, and C) (GNE-140) in MDA-MB-231 triple-negative breast cancer cells (TNBC) cells. The findings confirm that GNE-140 does in fact, fully block the production of lactic acid, which also results in a block of glucose utilization and severe impedance of the glycolytic pathway. Without a fully functional glycolytic pathway, breast cancer cells continue to thrive, sustain viability, produce ample energy, and maintain mitochondrial potential (ΔΨM). The only observable negative consequence of GNE-140 in this work, was the attenuation of cell division, evident in both 2D and 3D cultures and occurring in fully viable cells. Of important note, the cytostatic effects were not reversed by the addition of exogenous (+) lactic acid. While the effects of GNE-140 on the whole transcriptome were mild (12 up-regulated differential expressed genes (DEGs); 77 down-regulated DEGs) out of the 48,226 evaluated, the down-regulated DEGS collectively centered around a loss of genes related to mitosis, cell cycle, GO/G1–G1/S transition, and DNA replication. These data were also observed with digital florescence cytometry and flow cytometry, both corroborating a G0/G1 phase blockage. In conclusion, the findings in this work suggest there is an unknown element linking LDH enzyme activity to cell cycle progression, and this factor is completely independent of lactic acid. The data also establish that complete inhibition of LDH in cancer cells is not a detriment to cell viability or basic production of energy.

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Section: Discussionmentioning

confidence: 99%

Triple Isozyme Lactic Acid Dehydrogenase Inhibition in Fully Viable MDA-MB-231 Cells Induces Cytostatic Effects That Are Not Reversed by Exogenous Lactic Acid

et al. 2021

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“…Quantum dots (QDs) can go about as the primary nanocarrier or be important for additional perplexing structures. Paclitaxel (PTX), cancer-battling drug, is frequently bundled with nanostructured lipid transporters intended to have a theranostic way to deal with malignant growth treatment [49]. It was found to have exemplification adequacy of ~80%.…”

Section: Drug Delivery Applicationmentioning

confidence: 99%

Application of Quantum Dots in Bio-Sensing, Bio-Imaging, Drug Delivery, Anti-Bacterial Activity, Photo-Thermal, Photo-Dynamic Therapy, and Optoelectronic Devices

Rajamanickam¹

2023

Quantum Dots - Recent Advances, New Perspectives and Contemporary Applications

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Quantum dots (QDs) are of prevalent scientific and technological consideration because of their tunable size and thus frequency change (band-gap energy) in the NIR optical region. QDs have exceptional properties such as optical, physiochemical, electrical, and capacity to be bound to biomolecules. These selective size-dependent attributes of QDs assist them with having versatile applications in optoelectronic and biomedical fields. Their capacity to emit light at various frequencies because of an outer stimulus makes quantum dots perfect for use in imaging, diagnostics, tests for individual particles, and medication transportation frameworks. Ongoing advances in quantum dot design incorporate the potential for these nanocrystals to become therapeutic agents to restore numerous disease conditions themselves via bioconjugation with antibodies or medications. In this chapter, a few advances in the field of biomedical applications, such as bio-sensing, bio-imaging, drug loading capacity, targeted drug delivery, anti-stacking limit hostile to bacterial activity, photo-thermal treatment, photodynamic treatment, and optical properties for biomedical applications are presented, further to a short conversation on difficulties; for example, the biodistribution and harmful toxic effects of quantum dots is also discussed.

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“…2022, 22, x FOR PEER REVIEW 1 aptamer and activated the carboxy of nanohydrogels using Carbodiimide to induce th occurrence of the amidation reaction. The aptamers target MCF-7 cells of breast cance and assemble sodium oxamate and paclitaxel in the hydrogel to inhibit mitochondria function, giving the hydrogel the ability to specifically eradicate cancer cells [38]. Another typical hybrid assembly strategy is to use nucleic acids as crosslinkers.…”

Section: Hybrid Nahsmentioning

confidence: 99%

“…The aptamer-functional hydrogel is promising, which is functionalized by different aptamers after assembly and has a target specificity. Depending on the specificity of the aptamer, such hydrogels can achieve target delivery of a drug in the body [38]. Targetresponsiveness of the aptamer-hydrogel can also be reflected in the loading of the drug.…”

Section: Target-responsive Nahsmentioning

confidence: 99%

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Smart Nucleic Acid Hydrogels with High Stimuli-Responsiveness in Biomedical Fields

Zhang

Zhu

et al. 2022

IJMS

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Due to their hydrophilic, biocompatible and adjustability properties, hydrogels have received a lot of attention. The introduction of nucleic acids has made hydrogels highly stimuli-responsiveness and they have become a new generation of intelligent biomaterials. In this review, the development and utilization of smart nucleic acid hydrogels (NAHs) with a high stimulation responsiveness were elaborated systematically. We discussed NAHs with a high stimuli-responsiveness, including pure NAHs and hybrid NAHs. In particular, four stimulation factors of NAHs were described in details, including pH, ions, small molecular substances, and temperature. The research progress of nucleic acid hydrogels in biomedical applications in recent years is comprehensively discussed. Finally, the opportunities and challenges facing the future development of nucleic acid hydrogels are also discussed.

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MUC-1 aptamer conjugated InP/ZnS quantum dots/nanohydrogel fluorescent composite for mitochondria-mediated apoptosis in MCF-7 cells

Cited by 31 publications

References 56 publications

Triple Isozyme Lactic Acid Dehydrogenase Inhibition in Fully Viable MDA-MB-231 Cells Induces Cytostatic Effects That Are Not Reversed by Exogenous Lactic Acid

Triple Isozyme Lactic Acid Dehydrogenase Inhibition in Fully Viable MDA-MB-231 Cells Induces Cytostatic Effects That Are Not Reversed by Exogenous Lactic Acid

Application of Quantum Dots in Bio-Sensing, Bio-Imaging, Drug Delivery, Anti-Bacterial Activity, Photo-Thermal, Photo-Dynamic Therapy, and Optoelectronic Devices

Smart Nucleic Acid Hydrogels with High Stimuli-Responsiveness in Biomedical Fields

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