Oxidant-antioxidant status in Egyptian children with sickle cell anemia: a single center based study

El-Ghamrawy, Mona; Hanna, Wagdi Maurice; Abdelsalam, A.; El-Sonbaty, Marwa M.; Youness, Eman R.; Adel, Ahmed

doi:10.1016/j.jped.2013.09.005

Cited by 18 publications

(8 citation statements)

References 25 publications

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“…This observation is similar to previous reports from Nigeria [8], Egypt [9] and Qatar [5]. The decrease in antioxidant enzyme levels and activity could be explained by their susceptibility to ROS-induced oxidation [13, 14].…”

Section: Discussionsupporting

confidence: 91%

“…Although the use of antioxidants and supplementation of enzyme cofactors can be conceivably envisaged, the prominent renoprotective effect of angiotensin II type 1 receptor blocker (ARB) Telmisartan in type 2 diabetes, a condition associated with microalbuminuria as SCA has been reported to be mediated through enhancing antioxidant defense capacity and reducing oxidative stress [6]. Unfortunately, data on oxidative stress in SCA patients with and without microalbuminuria are very scare in Africa, a setting where SCA is prevailing [7–10]. In order to contribute to a better understanding of the physiopathology of glomerular damage in SCA, this work aimed to study the relationship between urinary albumin creatinine ratio (UACR) and enzymatic antioxidant status assessed using two markers: serum GPx and Cu-Zn SOD, in Steady state Black African children with SCA.…”

Section: Introductionmentioning

confidence: 99%

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Albuminuria, serum antioxidant enzyme levels and markers of hemolysis and inflammation in steady state children with sickle cell anemia

et al. 2016

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BackgroundOxidative stress is thought to be involved in the pathogenesis of microalbuminuria in Sickle cell anemia (SCA). Antioxidant enzymes such as glutathione peroxidase (GPx) and Cu-Zn superoxide dismutase (SOD) may play an important protective role. This study aimed to evaluate the association between albuminuria and these two antioxidant enzymes.MethodsWe consecutively recruited Steady state children aged between 2 and 18 years old with established diagnosis of homozygous SCA in two hospitals of Kinshasa/DR Congo. The relationship between Urinary Albumin Creatinine Ratio (UACR) and other variables of interest (age, systolic blood pressure, diastolic blood pressure, plasma GPx and Cu-Zn SOD, free plasmatic hemoglobin, LDH, indirect bilirubin, white blood cells (WBC), percentage of fetal hemoglobin, serum iron, ferritin, CRP) was analyzed by Bivariate correlation (Pearson’s correlation coefficient). Microalbuminuria was defined by urine albumin/creatinine ratio between 30 and 299 mg/g.ResultsSeventy Steady state Black African children with SCA (56% boys; average age 9.9 ± 4.3 years; 53% receiving hydroxyurea) were selected. Prevalence of microalbuminuria was 11.8%. LDH (r = 0.260; p = 0.033) and WBC count (r = 0.264; p = 0.033) were positively correlated with UACR whereas GPx (− 0.328; p = 0.007) and Cu-Zn SOD (− 0.210; p = 0.091) were negatively correlated with UACR.ConclusionsAlbuminuria is associated with decreased antioxidant capacity and increased levels of markers of hemolysis and inflammation. Therefore, strategies targeting the reduction of sickling and subsequent hemolysis, oxidative stress and inflammation could help preventing or at least delaying the progression of kidney disease in SCA children.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Albuminuria, serum antioxidant enzyme levels and markers of hemolysis and inflammation in steady state children with sickle cell anemia

et al. 2016

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“…HU is an FDA‐approved drug for the treatment of SCD through reduction in HbS polymerization and vaso‐occlusive crises by fetal hemoglobin augmentation . HU is suggested to produce direct oxidant and indirect antioxidant effects; however, there have been contradictory reports regarding the protective role of HU therapy in SCD patients . To examine the possible proactive role of HU treatment on oxidative imbalance in β‐thalassemia, we studied oxidative stress biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…Although the underlying mechanisms for the development of oxidative stress in SCD and β‐thalassemia are different, the oxidant/antioxidant ratio seems to be more affected in the latter . On the basis of thiobarbituric acid reactive substances (TBARS) and GSH analysis, a few studies reported an antioxidant role of HU in SCD subjects; however, investigations on MDA, PON1, 26 and vitamin E 26 have suggested that oxidative damage among SCD patients was independent of HU therapy. It was also argued that the observed discrepancy regarding antioxidant effects of HU therapy might be due to the short treatment period .…”

Section: Discussionmentioning

confidence: 99%

“…HU therapy has been shown to induce GSH concentrations and to reduce lipid peroxidation in SCD patients, thus acting as a protective agent against oxidative stress . However, there have been contradicting reports regarding the protective role of HU therapy on oxidative imbalance among SCD patients . This potential effect of HU on oxidative stress in β‐thalassemia subjects has not been investigated to date.…”

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confidence: 99%

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Serum Paraoxonase Activity and Malondialdehyde Serum Concentrations Remain Unaffected in Response to Hydroxyurea Therapy inβ-Thalassemia Patients

Zohaib

Ansari

Hashim

et al. 2015

The Journal of Clinical Pharmacology

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β-Thalassemia is the most common hereditary disorder characterized by reduced production of β-globin chains of hemoglobin A (HbA). In recent years, hydroxyurea (HU) has shown promising therapeutic benefits in patients with β-thalassemia by fetal hemoglobin augmentation. We have analyzed effects of hydroxyurea treatment on oxidative stress in β-thalassemia patients by assessing activities of paraoxonase (PON) and arylesterase along with malondialdehyde (MDA) and total reactive oxygen species (ROS) concentrations. Blood samples from 159 individuals including 56 HU-treated and 58 untreated β-thalassemia patients and 45 healthy controls were analyzed. PON activity was found to be highest in healthy individuals (177.76 ± 4.44 U/mL) as compared to treated (52.67 ± 3.65 U/mL) and untreated (55.11 ± 3.26 U/mL) patients. A similar trend was observed in the case of arylesterase activity in normal, β-thalassemia-treated, and untreated (210.0 ± 11.25 U/mL, 163.03 ± 9.04 U/mL, 139.77 ± 10.10 U/mL) subjects. Serum MDA concentrations (2.59 ± 0.09 nmol/mL, 2.45 ± 0.08 nmol/mL, and 1.15 ± 0.05 nmol/mL) and total ROS concentrations (3.73 ± 0.20 nmol/mL, 3.54 ± 0.23 nmol/mL, and 2.45 ± 0.14 nmol/mL) were significantly elevated in both groups (untreated and treated) as compared to healthy individuals (P < .01). Oxidative stress was found to be markedly elevated in β-thalassemia patients as compared to healthy controls. Insignificant differences were, however, observed in mean concentrations of PON1 paraoxonase and arylesterase activities, serum MDA concentration and total ROS concentrations between HU-treated and untreated patients. We propose that HU therapy alone seems to be ineffective in managing oxidative stress and is likely to offer a better clinical outcome when supplemented with efficient iron chelation therapy and antioxidants.

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Correlation of lipid peroxidation and nitric oxide metabolites, trace elements, and antioxidant enzymes in patients with sickle cell disease

Antwi‐Boasiako

Dankwah

Aryee

et al. 2020

Clinical Laboratory Analysis

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Background Lipid peroxidation plays a very important role in sickle cell pathophysiology. The formation of malondialdehyde (MDA) in patients with sickle cell disease (SCD) may lead to endothelial dysfunction. Nitric oxide (NO) is a known vasodilator which plays a role in endothelial function. The current study determined the association between MDA and NO metabolites (NOx), trace elements, and antioxidant enzymes (SOD and CAT) in patients with SCD. The ratio of MDA/NOx was also determined as an index of oxidative stress in the study groups. Methods This was a cross‐sectional study involving 90 patients with SCD and 50 “healthy” controls. Blood samples (n = 140) were collected from the study groups. The plasma, sera, and red cells were kept at −20°C for biochemical analyses. Hemoglobin (Hb) and NOx levels were determined in the plasma using Labsystem Multiskan MS and Griess reagent system, respectively. Super oxide dismutase (SOD) and catalase (CAT) levels were determined in the red cells using assay kits from Cayman chemicals. Lipid peroxidation biomarker MDA was determined in the sera using the TBARS assay. Levels of iron (Fe), copper (Cu), and zinc (Zn) were also determined in the sera using Variant 240FS. MDA and NOx ratio was computed for the study groups and compared. Results Levels of Hb, NOx, SOD, CAT, and Zn were significantly lower in the patients with SCD ( P < .001). MDA, Fe, and MDA/ NOx ratio were, however, significantly higher in the patients with SCD ( P < .001). There was no significant correlation between MDA and NOx, SOD, CAT, Fe, and Zn in the study groups. MDA, however, correlated positively and significantly with Cu in the HbSS patients with vaso‐occlusive crises (VOC). Gender did not affect the levels of oxidative stress markers. Conclusions Findings from this study suggest a link between lipid peroxidation and Cu in HbSS patients with VOC. Increased MDA/NOx ratio may contribute to sickle cell pathophysiology by promoting oxidative stress.

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Oxidant-antioxidant status in Egyptian children with sickle cell anemia: a single center based study

Cited by 18 publications

References 25 publications

Albuminuria, serum antioxidant enzyme levels and markers of hemolysis and inflammation in steady state children with sickle cell anemia

Albuminuria, serum antioxidant enzyme levels and markers of hemolysis and inflammation in steady state children with sickle cell anemia

Serum Paraoxonase Activity and Malondialdehyde Serum Concentrations Remain Unaffected in Response to Hydroxyurea Therapy inβ-Thalassemia Patients

Correlation of lipid peroxidation and nitric oxide metabolites, trace elements, and antioxidant enzymes in patients with sickle cell disease

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