Design and evaluation of bioenhanced oral tablets of Dunaliella salina microalgae for treatment of liver fibrosis

El-Baz, Farouk; Ali, Sami I.; Basha, Mona; Kassem, Ahmed Alaa; Shamma, Rehab Nabil; Elgohary, Rania; Salama, Abeer

doi:10.1016/j.jddst.2020.101845

Cited by 10 publications

(6 citation statements)

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“…Further evidence has shown that astaxanthin (80 mg/kg), during liver fibrosis, inhibited the expression of TGF- β and activation of HSCs [ 51 , 52 ]. Also, β -carotene from D. salina treating liver fibrosis via decrement of α -SMA and COL-1 liver contents [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…Besides, astaxanthin and astaxanthin esters H. pluvialis microalgae synthesized β -carotene [ 16 ]. As a result of their potent competency to scavenge different reactive oxygen species and quench singlet oxygen, the antioxidant capability of different carotenoid individuals, including astaxanthin and β -carotene, is of prominent importance to human health [ 17 ] through their ameliorative ability against oxidative stress; the main promoter of many diseases including liver disorders [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…β -Carotene also has anti-inflammatory potentials by suppressing different proinflammatory markers including redox-based nuclear factor-kappa- β (NF- κβ ), NADPH oxidase protein, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) was reported [ 25 , 26 ]. Also, β -carotene from other algae Dunaliella salina (D. salina) has an improvement potential against liver fibrosis injures induced by thioacetamide (TAA) in rats [ 19 ]. In this study, we evaluated the therapeutic effect of H. pluvialis extract and its astaxanthin-rich fraction, astaxanthin ester-rich fraction, and β -carotene-rich fraction and their mechanisms of action in curing hepatic fibrosis induced by TAA in rats via modulation of MMP and its inhibitor as a target to ameliorate liver fibrosis.…”

Section: Introductionmentioning

confidence: 99%

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Haematococcus pluvialis Carotenoids Enrich Fractions Ameliorate Liver Fibrosis Induced by Thioacetamide in Rats: Modulation of Metalloproteinase and Its Inhibitor

Baz

Salama

Ali

et al. 2021

BioMed Research International

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Hepatic fibrosis is a consequence of chronic liver diseases. Metalloproteinase and its inhibitor have crucial roles in the resolution of liver fibrosis. The current relevant study is aimed to evaluate the therapeutic effect of Haematococcus pluvialis (H. pluvialis) extract, astaxanthin-rich fraction, astaxanthin ester-rich fraction, and β-carotene-rich fraction as well as their mechanisms of action in curing hepatic fibrosis induced by thioacetamide (TAA). Liver fibrosis was induced using TAA (intraperitoneal injection, two times a week for 6 weeks), in a rat model and H. pluvialis extract (200 mg/kg), and other fractions (30 mg/kg) were orally administered daily for 4 weeks after the last TAA injection. Based on HPLC analysis, H. pluvialis extract contains β-carotene (12.95 mg/g, extract) and free astaxanthin (10.85 mg/g, extract), while HPLC/ESI-MS analysis revealed that H. pluvialis extract contains 28 carotenoid compounds including three isomers of free astaxanthin, α or β-carotene, lutein, 14 astaxanthin mono-esters, 5 astaxanthin di-esters, and other carotenoids. H. pluvialis and its fractions reduced liver enzymes, nitric oxide, collagen 1, alpha-smooth muscle actin, and transforming growth factor-beta as well as elevated catalase antioxidant activity compared to the TAA group. Also, H. pluvialis extract and its fractions exceedingly controlled the balance between metalloproteinase and its inhibitor, activated Kupffer cells proliferation, and suppressed liver apoptosis, necrobiosis, and fibrosis. These findings conclude that H. pluvialis extract and its fractions have an antifibrotic effect against TAA-induced liver fibrosis by regulating the oxidative stress and proinflammatory mediators, suppressing multiple profibrogenic factors, and modulating the metalloproteinase and its inhibitor pathway, recommending H. pluvialis extract and its fractions for the development of new effective medicine for treating hepatic fibrosis disorders.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Haematococcus pluvialis Carotenoids Enrich Fractions Ameliorate Liver Fibrosis Induced by Thioacetamide in Rats: Modulation of Metalloproteinase and Its Inhibitor

Baz

Salama

Ali

et al. 2021

BioMed Research International

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“…The disintegration test is said to be complete when no palatable mass remains on the surface of the screen. [15] The results are displayed in Table 6.…”

Section: Evaluation Of Post-compression Parametersmentioning

confidence: 99%

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Biju¹

2021

AJP

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Introduction: Clomiphene citrate is a nonsteroidal ovulation inducer used in the treatment of infertility. This drug belongs to biopharmaceutical classification system Class-II often possesses challenges concerning solubility or poor dissolution, drug release, and thereby enhance the bioavailability. Aim: The aim of the study was to improve the solubility of the drug as well as to make the formulation more feasible liquisolid technique was adopted. Materials and Methods: Here, Avicel PH 102(Q) and Aerosil 200(q) in varying ratios ranging from 5, 10, 20, and 25 were employed as coating and carrier materials (R=Q/q), and their quantities incorporated into formulation were calculated based on the mathematical formulae developed by Spireas and final powder substrate developed was compressed into a tablet by direct compression method. Twelve formulations LS1 to LS12 were prepared and subjected to drug excipient interactions studies, pre-compression, and post-compression studies and compared with the marketed formulation. Results and Discussion: The Fourier transform infrared spectroscopy results showed no interaction between drug-excipients. Scanning electron microscopy and X-ray diffractometry analysis indicated that the clomiphene citrate is held within the powder substrate in a solubilized, almost molecularly dispersed state and in liquisolid as an amorphous powder with no signs of instability on storage. An optimized formulation was selected based on the in vitro, drug release studies. LS2 formulation containing Avicel PH 102(Q):Aerosil 200(q) a ratio of 10:1 and drug concentration 10% exhibited the controlled and complete/highest drug release rate of 96.46% in 30 min in comparison with the marketed product. Conclusion: Thus, we propose that liquisolid technique can be chosen as the most economical and alternative approach to enhance the solubility of clomiphene citrate.

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“…[ 24 ] Moreover, D. salina revealed a significant antifibrotic effect in induced‐fibrosis rats via ameliorating the elevation of liver enzymes, inflammatory mediators, and fibrotic markers. [ 25 ] In addition, β‐carotene is transformed into retinol (vitamin A) in the intestine and other organs, which is an essential vitamin with numerous biological functions [ 26 ]…”

Section: Introductionmentioning

confidence: 99%

Effect of the Emulsifier Used in Dunaliella salina‐Based Nanoemulsions Formulation on the β‐Carotene Absorption and Metabolism in Rats

Teixé-Roig

Oms‐Oliu

Odriozola-Serrano

et al. 2023

Molecular Nutrition Food Res

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Scope: Microalgae such as Dunaliella salina are a potential sustainable source of natural 𝜷-carotene due to their fast growth and high adaptability to environmental conditions. This work aims to evaluate the effect of the incorporation of 𝜷-carotene from this alga into different emulsifier-type nanoemulsions (soybean lecithin [SBL], whey protein isolate [WPI], sodium caseinate [SDC]) on its absorption, metabolization, and biodistribution in rats. Methods and results: Nanoemulsions formulated with different emulsifiers at 8% concentration are obtained by five cycles of microfluidization at 130 mPa, then expose to an in vitro digestion or orally administer to rats. Feeding rats with nanoemulsions improves 𝜷-carotene uptake compared to control suspension, especially using SDC and WPI as emulsifiers. A greater presence of 𝜷-carotene and retinol in the intestine, plasma, and liver is observed, being the liver the tissue that shows the highest accumulation. This fact can be a consequence of the smaller droplets that protein-nanoemulsions present compared to that with SBL in the intestine of rats, which promote faster digestibility and higher 𝜷-carotene bioaccessibility (35%-50% more) according to the in vitro observations. Conclusions: Nanoemulsions, especially those formulated with protein emulsifiers, are effective systems for increasing 𝜷-carotene absorption, as well as retinol concentration in different rat tissues.

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Design and evaluation of bioenhanced oral tablets of Dunaliella salina microalgae for treatment of liver fibrosis

Cited by 10 publications

References 59 publications

Haematococcus pluvialis Carotenoids Enrich Fractions Ameliorate Liver Fibrosis Induced by Thioacetamide in Rats: Modulation of Metalloproteinase and Its Inhibitor

Haematococcus pluvialis Carotenoids Enrich Fractions Ameliorate Liver Fibrosis Induced by Thioacetamide in Rats: Modulation of Metalloproteinase and Its Inhibitor

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Effect of the Emulsifier Used in Dunaliella salina‐Based Nanoemulsions Formulation on the β‐Carotene Absorption and Metabolism in Rats

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