Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus)

Abstract

Objective To investigate the effects of hydroalcoholic extract of Ziziphus jujuba on the histopathological, tissue oxidative stress and inflammation plus to antioxidant pathways of colon tissue in rat with induced Ulcerative colitis. Materials and methods Ulcerative colitis was induced in 80 rats those divided into 8 equal groups. Group 1 and 2 were negative controls receiving 1 mL/day of normal saline in enema and oral; group 3 and 4 as positive control 1 and 2 received 10 mg/kg of intra-colonic asacol and oral mesalazine; groups 5 and 6 received 20% and 40% of hydroalcoholic extract of Z. jujuba trans-rectally; group 7 and 8 received 1500 and 3000 mg/kg of hydroalcoholic extract of Z. jujuba orally, respectively. After 7 days, animals were evaluated for colon tissue histopathology, levels of malondialdehyde and IL-1β, and activities of superoxide dismutase, glutathione peroxidase and myeloperoxidase in colon tissue. Results Hydroalcoholic extract of Z. jujuba in both forms of trans-rectal and oral administration especially in the higher doses could result into a more healing effect in damaged colonic tissue, more reduce glutathione peroxidase and IL-1β level. Also, these two doses (gel 40% and oral 3000 mg/kg) could more decrease the myeloperoxidase activity and stimulate superoxide dismutase and glutathione peroxidase activities. Also, gel 40% in transrectal administration was more potent than administration 3000 mg/kg in oral. Conclusion The results of the present study indicated that Z. jujube may be considered as a treatment of choice for Ulcerative colitis especially in gel form and also in dose-dependent pattern.

Synergistic effects of hydro extract of jujube fruit in combination with Mesalazine (orally) and Asacol (intra-colonic) administration in ameliorating animal model of ulcerative colitis

“…Their studies are different from ours in that they used different doses of jujube extract and medications, measuring other cytokines. In other words, in their work, they either examined the impact of jujube on other diseases, the impact of other plants on induced colitis or measured other inflammatory indices 15,27 ; hence, we compare our results with similar parts of other studies in the following section.…”

“…25 Also, Zhao et al used the same method to examine the impact of magnolia on Dextran sulfate sodiuminduced colitis. 26 However, Karawya et al (Karawya et al, 2016) and Tanideh et al, 15 in their separate studies in 2016, examined the impact of jujube on acetic acid-induced colitis in rats. Their studies are different from ours in that they used different doses of jujube extract and medications, measuring other cytokines.…”

“…We used the same induction method as Hemayatkhah et al, 28 Ahmadinejad et al, 29 Koohpeyma et al, 30 Karawya et al, 28 and Tanideh et al 15 employed in their studies, which were respectively conducted in 2010, 2014, and 2016, to examine the impacts of milk thistle, chamomile, dracocephalum polychaetum, and jujube on acetic acid-induced large intestine ulcers. Other available methods for induced colitis studies include Dextran sulfate sodium-induced colitis (DSS) and trinitrobenzene sulfonic acid (TNBS).…”

“…The absorbance of the samples was measured at 460 nm by UV-visible spectrophotometer and the activity of MPO was calculated using a standard curve. 15…”

“…Using ELISA test, this study bought kits made by Bender Medsystems from Australia and used them -fully obeying the instructions-to measure the serum levels of supernatant cells' TNF-6 and IL-6 resulted by the homogenization of colon tissue in study rats. 15…”