A review of experimental models in colorectal carcinogenesis

Machado, Vanessa Foresto; Feitosa, Marley Ribeiro; Rocha, José Joaquim Ribeiro da; Féres, Omar

doi:10.1016/j.jcol.2015.09.001

Cited by 9 publications

(11 citation statements)

References 19 publications

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“…Besides adenomas, hyperplastic polyps, serrated adenomas, flat adenomas, and dysplastic lesions are also observed in the colon as other types of preneoplastic lesions [ 5 ]. In humans, colorectal cancer is histologically classified as an adenocarcinoma [ 4 , 6 ]. In Figure 1 we can observe the progression from normal intestinal epithelium to carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Animal Models of Colorectal Cancer: From Spontaneous to Genetically Engineered Models and Their Applications

Nascimento-Gonçalves

Mendes

Silva-Reis

et al. 2021

Veterinary Sciences

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Colorectal cancer is one of the most common gastrointestinal malignancies in humans, affecting approximately 1.8 million people worldwide. This disease has a major social impact and high treatment costs. Animal models allow us to understand and follow the colon cancer progression; thus, in vivo studies are essential to improve and discover new ways of prevention and treatment. Dietary natural products have been under investigation for better and natural prevention, envisioning to show their potential. This manuscript intends to provide the readers a review of rodent colorectal cancer models available in the literature, highlighting their advantages and disadvantages, as well as their potential in the evaluation of several drugs and natural compounds’ effects on colorectal cancer.

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Section: Introductionmentioning

confidence: 99%

Animal Models of Colorectal Cancer: From Spontaneous to Genetically Engineered Models and Their Applications

Nascimento-Gonçalves

Mendes

Silva-Reis

et al. 2021

Veterinary Sciences

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“…The discovery of chemical substances with carcinogenic properties has allowed the development of several experimental models of colorectal cancer 4 . These compounds, when administered to rodents, produce both benign and malignant colon neoplasms that are similar, in various aspects, to human tumors 5 - 7 .…”

Section: Introductionmentioning

confidence: 99%

Experimental Model of Rectal Carcinogenesis Induced By N-Methyl-N-Nitrosoguanidine In Mice with Endoscopic Evaluation

Machado¹,

Parra²,

Leite³

et al. 2020

Int. J. Med. Sci.

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Background and purpose: The discovery of chemical substances with carcinogenic properties has allowed the development of several experimental models of colorectal cancer (CRC). Classically, experimental models of CRC in mice have been evaluated through clinical or serial euthanasia. The present study aims to investigate the role of low endoscopy in the analysis of carcinogenesis induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). Methods: Thirty C57BL6 mice were divided into two groups: a control group with fifteen animals that underwent rectal instillation of saline solution on day 0 and a carcinogen group with fifteen animals that underwent a 100 mg/kg MNNG rectal instillation on day 0. In both groups, low endoscopies were performed on weeks 4 and 8. We used a validated endoscopic scoring system to evaluate the severity of colitis and colorectal tumor. Euthanasia was carried out at week 12. Results: We observed higher inflammation scores (p <0.001) and a higher number of tumors (p <0.05) in the MNNG group than the control group, both at weeks 4 and 8. A worsening of inflammation scores from the first to the second endoscopy was also noticeable in the MNNG group. There were no bowel perforations related to the procedure, and there was one death in the control group. Conclusion: Low endoscopy in experimental animals allows safe macroscopic evaluation of colorectal carcinogenesis without the need for euthanasia.

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“…D. pentandra has been shown to possess potent anticancer activity. Additionally, in Indonesia it is used to treat hypertension, diabetes, coughs, and as a diuretic [ 1 – 3 ]. Phytochemical studies have demonstrated that flavonoids are the main active fraction of D. pentandra leaves.…”

Section: Introductionmentioning

confidence: 99%

“…There are limitations in both efficacy and safety of current treatment for inflammatory bowel disease (IBD). Previous studies have shown that CD4 + T helper Th1 and Th2 cells are essential in the pathogenesis of IBD [ 2 , 3 ]. Innate and adaptive immunity of the host is thought to be involved in the pathogenesis of chronic colitis.…”

Section: Introductionmentioning

confidence: 99%

Extract from mango mistletoes Dendrophthoe pentandra ameliorates TNBS-induced colitis by regulating CD4+ T cells in mesenteric lymph nodes

Endharti

Permana

2017

BMC Complement Altern Med

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BackgroundMango mistletoes Dendrophthoe pentandra (MMDP) extract has attracted interest due to its pharmacological properties, including gastro protective effects. The aim of this study was to investigate whether MMDP extract could increase Foxp3 regulatory T cells and inhibits development of Th17 cells.MethodsColitis was induced in Balb/c mice by rectal administration of 2,4,6-trinitrobenzene sulfonic acid (TNBS). The mice were randomly divided into five groups comprising group1 receiving vehicle (the negative control), group 2–5 receiving TNBS, group 3–5 orally receiving either MMDP extract 150, 300 and 600 mg/kgBW for 7 days after TNBS administration. On day 8 of the experiment, the colon tissues were removed for histological examination, cytokine and myeloperoxidase (MPO) measurement. T-cells sub-population in mesenteric lymph nodes were analyzed by flow cytometer.ResultsMMDP extract potently suppressed colon shortening and MPO in mice with TNBS-induced colitis. Administration of the extract significantly decreased the severity of TNBS-induced colitis in a dose-dependent manner. The extract significantly attenuated the loss of body weight (p < 0.05). These effects were associated with a remarkable amelioration of the disruption of the colonic architecture, significant reduction of the colonic MPO (p < 0.05). The extract lowered the levels of Th17-associated cytokines but increased the production of Treg-associated cytokines in mesenteric lymph node cells.ConclusionOur results suggest that MMDP has the therapeutic potential to ameliorate TNBS-induced colitis symptoms revealed by histological change and inhibit IL-17 production.

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A review of experimental models in colorectal carcinogenesis

Cited by 9 publications

References 19 publications

Animal Models of Colorectal Cancer: From Spontaneous to Genetically Engineered Models and Their Applications

Animal Models of Colorectal Cancer: From Spontaneous to Genetically Engineered Models and Their Applications

Experimental Model of Rectal Carcinogenesis Induced By N-Methyl-N-Nitrosoguanidine In Mice with Endoscopic Evaluation

Extract from mango mistletoes Dendrophthoe pentandra ameliorates TNBS-induced colitis by regulating CD4+ T cells in mesenteric lymph nodes

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