Fabrication of vitamin E-enriched nanoemulsions: Factors affecting particle size using spontaneous emulsification

Saberi, Amir Hossein; Yuan, Fang; McClements, David Julian

doi:10.1016/j.jcis.2012.08.069

Cited by 369 publications

(270 citation statements)

References 55 publications

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“…This was performed in order to facilitate PSO solubilization and the formation of the droplets. Furthermore, according to the literature, the spontaneity of the emulsification can be affected by interfacial tension, bulk viscosity of oil phase, water miscible solvents and surfactant structure and/ or concentration (Saberi et al 2013). Droplet sizes were below 250 nm and PDI values were less than 0.2, which is compatible with other studies using the same method of preparation (Martini et al 2007;Ferreira et al 2015).…”

Section: Discussionsupporting

confidence: 85%

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Ferreira

Gehrcke

Cervi

et al. 2016

Pharmaceutical Biology

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(2016) Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells, Pharmaceutical Biology, 54:12, 2968-2977, DOI: 10.1080/13880209.2016 Context: Glioma is a malignant brain tumor with rapid proliferation, infiltrative growth, poor prognosis and it is chemoresistent. Pomegranate seed oil (PSO) has antioxidant, anti-inflammatory and antitumor properties. This study showed the optimization of PSO nanoemulsions (NEs) as an alternative for glioma treatment.Objective: The study aimed to evaluate PSO NEs cytotoxicity on human blood cells and antiglioma effects against C6 cells. Materials and methods: NEs were prepared by the spontaneous emulsification method, using PSO at 1.5 and 3.0%, and were evaluated regarding their physical stability and antioxidant activity. Toxicity evaluations in human blood cells were performed in terms of cell viability, genotoxicity, lipid peroxidation, protein carbonylation, catalase activity and hemolysis at 0.1, 0.25 and 0.5 mg/mL PSO, after a 72-h incubation period. In vitro antitumor effect was determined against glioma cells after 24 and 48 h, and astrocytes were used as a non-transformed cell model. Results: Formulations presented droplet size below 250 nm, low polydispersity index, negative zeta potential and pH in the acid range. NEs and PSO had scavenging capacity around 30% and promoted a proliferative effect in mononuclear cells, increasing about 50% cell viability. No genotoxic and oxidative damage was observed in lipid peroxidation, protein carbonylation and catalase activity evaluations for NEs. Hemolysis study showed a hemolytic effect at high concentrations. Moreover, formulations reduced only tumor cell viability to 47%, approximately. Discussion and conclusion: Formulations are adequate and safe for intravenous administration. Besides, in vitro antitumor activity indicates that NEs are promising for glioma treatment.ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 85%

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Ferreira

Gehrcke

Cervi

et al. 2016

Pharmaceutical Biology

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“…Droplet size was effectively reduced from 83.96 nm to 11.93 nm upon an increase in mixing rate from 320 rpm to 640 rpm, which corresponded to previous results. 54 The width of the particle-size distribution was not strongly influenced by mixing rate, as described by Saberi et al 55 Similar results were observed for emulsion-droplet size and mixing rate for emulsification of nanoemulsions. 53,56 Previous studies 55,57 suggested that gentle mixing was needed to form very fine droplets, highlighting the importance of controlling this parameter.…”

supporting

confidence: 77%

Formulation, development, and optimization of a novel octyldodecanol-based nanoemulsion for transdermal delivery of ceramide IIIB

Su¹,

Yang²,

Wang³

et al. 2017

IJN

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This research aimed to develop and optimize a nanoemulsion-based formulation containing ceramide IIIB using phase-inversion composition for transdermal delivery. The effects of ethanol, propylene glycol (PG), and glycerol in octyldodecanol and Tween 80 systems on the size of the nanoemulsion region in the phase diagrams were investigated using water titration. Subsequently, ceramide IIIB loading was kept constant (0.05 wt%), and the proposed formulation and conditions were optimized via preliminary screening and experimental design. Factors such as octyldodecanol/(Tween 80:glycerol) weight ratio, water content, temperature, addition rate, and mixing rate were investigated in the preliminary screening experiment. Response surface methodology was employed to study the effect of water content (30%–70%, w/w), mixing rate (400–720 rpm), temperature (20°C–60°C), and addition rate (0.3–1.8 mL/min) on droplet size and polydispersity index. The mathematical model showed that the optimum formulation and conditions for preparation of ceramide IIIB nanoemulsion with desirable criteria were a temperature of 41.49°C, addition rate of 1.74 mL/min, water content of 55.08 wt%, and mixing rate of 720 rpm. Under optimum formulation conditions, the corresponding predicted response values for droplet size and polydispersity index were 15.51 nm and 0.12, respectively, which showed excellent agreement with the actual values (15.8 nm and 0.108, respectively), with no significant ( P >0.05) differences.

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“…Thermal methods rely on properties with temperature, whereas isothermal methods rely on emulsion formation resulting from changes in local system composition at a fixed temperature 12 . Spontaneous emulsification and phase inversion composition methods fall into the category of isothermal methods 13 , while the phase inversion temperature method is an example of a thermal method 14,15 .…”

Section: Introductionmentioning

confidence: 99%

O/W Nano-Emulsion Formation Using an Isothermal Low-Energy Emulsification Method in a Mixture of Polyglycerol Polyricinoleate and Hexaglycerol Monolaurate with Glycerol System

Wakisaka

Nishimura²,

Gohtani

2015

J. Oleo Sci.

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Fabrication of vitamin E-enriched nanoemulsions: Factors affecting particle size using spontaneous emulsification

Cited by 369 publications

References 55 publications

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells

Formulation, development, and optimization of a novel octyldodecanol-based nanoemulsion for transdermal delivery of ceramide IIIB

O/W Nano-Emulsion Formation Using an Isothermal Low-Energy Emulsification Method in a Mixture of Polyglycerol Polyricinoleate and Hexaglycerol Monolaurate with Glycerol System

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