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Cited by 51 publications
(35 citation statements)
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References 19 publications
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“…Both samples were diluted in 10 mM HEPES, pH 7.4, 150 mM NaCl, 5% glycerol, 0.75 mM Tris(2-carboxyethyl)phosphine, 2.5% acetonitrile, and 0.05% formic acid and loaded to a CapLC system TM and Q-Tof TM quadrupole/orthogonal acceleration time-of-flight mass spectrometer (Waters Corp., Manchester) using the method described by Sundqvist et al (24). The spectra were combined and deconvoluted to zero charged ions with the maximum Entrophy TM 1 algorithm in Masslynx software (Waters Corp., Milford, MA).…”
Section: Methodsmentioning
confidence: 99%
“…Both samples were diluted in 10 mM HEPES, pH 7.4, 150 mM NaCl, 5% glycerol, 0.75 mM Tris(2-carboxyethyl)phosphine, 2.5% acetonitrile, and 0.05% formic acid and loaded to a CapLC system TM and Q-Tof TM quadrupole/orthogonal acceleration time-of-flight mass spectrometer (Waters Corp., Manchester) using the method described by Sundqvist et al (24). The spectra were combined and deconvoluted to zero charged ions with the maximum Entrophy TM 1 algorithm in Masslynx software (Waters Corp., Milford, MA).…”
Section: Methodsmentioning
confidence: 99%
“…Protein Mass Spectrometry-A Q-Tof TM II quadrupole/orthogonal acceleration time-of-flight mass spectrometer coupled on-line to a CapLC TM liquid chromatography system (Waters Corp./Micromass MS Technologies, Manchester, UK) was used for protein MS analysis essentially as described (26). Samples were prepared in 5% aqueous acetonitrile containing 0.1% formic acid as described above.…”
Section: Methodsmentioning
confidence: 99%
“…Protein concentrations were then determined using Epoch Micro-Volume Spectrophotometer System (BioTek Inc., Winooski, VT, USA) at 280 nm, and the identities of the expressed proteins were confirmed by intact MS. Overexpression and purification of the active site mutants, CjGH74(D70A) and CjGH74(D483A), were conducted with the same protocol used for the wild-type enzyme. The fidelity of protein production was confirmed by intact protein MS [84].…”
Section: Gene Expression and Protein Purificationmentioning
confidence: 97%