Direct electrochemistry of glucose oxidase assembled on graphene and application to glucose detection

Wu, Ping; Shao, Qian; Yao, Hang; Jin, Juan; Yin, Yaozu; Zhang, Hui; Cai, Chenxin

doi:10.1016/j.electacta.2010.07.079

Cited by 236 publications

(120 citation statements)

References 56 publications

(72 reference statements)

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“…This electrocatalytic process toward the reduction of dissolved oxygen may be expressed as follows: [6,35,37] GOx(FAD) + 2e…”

Section: Electrocatalytical Behavior Of the Fementioning

confidence: 99%

Magnetic Fe3O4-Reduced Graphene Oxide Nanocomposites-Based Electrochemical Biosensing

et al. 2014

Nano-Micro Lett

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An electrochemical biosensing platform was developed based on glucose oxidase (GOx)/Fe3O4-reduced graphene oxide (Fe3O4-RGO) nanosheets loaded on the magnetic glassy carbon electrode (MGCE). With the advantages of the magnetism, conductivity and biocompatibility of the Fe3O4-RGO nanosheets, the nanocomposites could be facilely adhered to the electrode surface by magnetically controllable assembling and beneficial to achieve the direct redox reactions and electrocatalytic behaviors of GOx immobilized into the nanocomposites. The biosensor exhibited good electrocatalytic activity, high sensitivity and stability. The current response is linear over glucose concentration ranging from 0.05 to 1.5 mM with a low detection limit of 0.15 μM. Meanwhile, validation of the applicability of the biosensor was carried out by determining glucose in serum samples. The proposed protocol is simple, inexpensive and convenient, which shows great potential in biosensing application.

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“…This electrocatalytic process toward the reduction of dissolved oxygen may be expressed as follows: [6,35,37] GOx(FAD) + 2e…”

Section: Electrocatalytical Behavior Of the Fementioning

confidence: 99%

Magnetic Fe3O4-Reduced Graphene Oxide Nanocomposites-Based Electrochemical Biosensing

et al. 2014

Nano-Micro Lett

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“…19 Several reports of carbon composite materials, such as graphene-gold nanoparticles-chitosan hybrid film and glucose oxidase-assembled graphene and graphene oxidePrussian blue hybrid film for glucose biosensing have been published. [19][20][21] Although graphene-based electrodes and other electrodes modified by composite materials have demonstrated their utility as electrochemical glucose biosensors in phosphate-buffered saline, and serum and urine samples, 3,[19][20][21][22] to the best of our knowledge, their use for detection of glucose in urine and serum samples from diabetic patients using a functionalized electrode has not yet been studied. Furthermore, the graphene-based composites utilized so far for electrochemical studies have been fabricated by physical modification 19 and are not chemically bonded or functionalized, suggesting lack of long-term binding affinity and stability problems between nanoparticles and graphene oxide during electrochemical analysis.…”

Section: Introductionmentioning

confidence: 99%

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Veerapandian¹,

Seo²,

Shin³

et al. 2012

IJN

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Abstract:A novel clinical glucose biosensor fabricated using functionalized metalloid-polymer (silver-silica coated with polyethylene glycol) hybrid nanoparticles on the surface of a graphene oxide nanosheet is reported. The cyclic voltammetric response of glucose oxidase modification on the surface of a functionalized graphene oxide electrode showed a surface-confined reaction and an effective redox potential near zero volts, with a wide linearity of 0.1-20 mM and a sensitivity of 7.66 µA mM. The functionalized graphene oxide electrode showed a better electrocatalytic response toward oxidation of H 2 O 2 and reduction of oxygen. The practical applicability of the functionalized graphene oxide electrode was demonstrated by measuring the peak current against multiple urine and serum samples from diabetic patients. This new hybrid nanoarchitecture combining a three-dimensional metalloid-polymer hybrid and two-dimensional graphene oxide provided a thin solid laminate on the electrode surface. The easy fabrication process and retention of bioactive immobilized enzymes on the functionalized graphene oxide electrode could potentially be extended to detection of other biomolecules, and have broad applications in electrochemical biosensing.

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“…Our electrode shows sensitivity and LOD similar to results by Palanisamy et al 18 which used thick GO hybridized with MWCNT and supported by glassy carbon electrode (GCE) that demonstrated high thermodynamic surface adsorption 59 and specific thermodynamics adsorption and redox reduction mechanism with GOx. 60 Although our electrode underperformed those with thick RGO, 11,18,28,29,53 it demonstrated the feasibility of immobilizing GOx onto monolayer RGO for glucose sensors. The LOD of our electrodes outperformed those reported previously for GOx immobilized hybrids of AuNPs-RGO 56 and CdS-RGO 57 on GCE and RGO-IL on modified Au.…”

Section: Resultsmentioning

confidence: 99%

“…The sensitivity and LOD of the fabricated electrode are compared to various electrodes as shown in Table II. 11,18,28,29,[53][54][55][56][57][58] Most of the electrodes in the review have high sensitivity and good LOD as they used thick GO prepared by a modified Hummers method. Our electrode shows sensitivity and LOD similar to results by Palanisamy et al 18 which used thick GO hybridized with MWCNT and supported by glassy carbon electrode (GCE) that demonstrated high thermodynamic surface adsorption 59 and specific thermodynamics adsorption and redox reduction mechanism with GOx.…”

Section: Resultsmentioning

confidence: 99%

“…In amperometric biosensors for glucose detection, GOx is immobilized on a nanomaterial surface and catalytically oxidizes glucose for high sensitivity and selectivity. [9][10][11] However, enzyme immobilization is complicated by poor electrical communication of the active sites of the enzyme and the electrode surface, and by enzyme leaching. [12][13][14][15] Recently, graphene has been used to modify biosensors, offering advantages for enzyme immobilization on graphene surfaces.…”

mentioning

confidence: 99%

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Functionalization of CVD Grown Graphene with Downstream Oxygen Plasma Treatment for Glucose Sensors

Hadish

Jou

Huang

et al. 2017

J. Electrochem. Soc.

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The development of graphene-based devices requires control over the functionalization and modification of the graphene surface. We studied the modification of the monolayer graphene surface with downstream oxygen plasma treatment to form graphene oxide (GO) for use in glucose biosensors. Amperometric and voltammetric methods were applied to GO for glucose oxidase (GOx) immobilization with a simultaneous reduction of GO to reduce graphene oxide. The fabricated glucose sensor shows a sensitivity of 0.118 μA mM −1 cm −2 and a detection limit of 0.0526 mM of glucose. This method provides fast and simultaneous immobilization of GOx and reduction of GO, and can be used in the fabrication of other electrochemical biosensors. Glucose oxidase has been recognized as an enzyme for monitoring glucose. In amperometric biosensors for glucose detection, GOx is immobilized on a nanomaterial surface and catalytically oxidizes glucose for high sensitivity and selectivity.9-11 However, enzyme immobilization is complicated by poor electrical communication of the active sites of the enzyme and the electrode surface, and by enzyme leaching. [12][13][14][15] Recently, graphene has been used to modify biosensors, offering advantages for enzyme immobilization on graphene surfaces. [16][17][18] Pristine graphene (PrG), a monolayer structure composed of one-atom thick two-dimensional honeycomb lattices of sp 2 carbon atoms, has extraordinary properties such as high intrinsic mobility and excellent thermal and electrical conductivity. [19][20][21][22] Although the bulk properties of graphene are very promising for a variety of applications, the development of graphene-based devices requires great control over the functionalization and modification of the graphene surface. Graphene oxide (GO) is one branch of functionalized graphene. GO is typically covalently functionalized with hydroxyl, epoxy, carbonyl, and carboxyl groups on its basal planes and at its edges, 23 resulting in a hybrid structure comprising a mixture of sp 2 and sp 3 hybridized carbon atoms. 24 GO sheets synthesized from graphite powders by the modified Hummers method 25 have been coated on carbon and Pt electrodes and used to immobilize GOx to develop glucose sensors. [26][27][28][29] On the other hand, monolayer graphene has been fabricated by chemical vapor deposition (CVD) on Cu and Ni substrates then transferred to other substrates for chemical and gas sensors. [30][31][32] Monolayer graphene has also been utilized for glucose sensor after immobilizing GOx to its surface through linker molecules. 33,34 With the aid of lithography and plasma etch techniques, monolayer-graphene-based devices can be patterned into arrays 32 and integrated with Si microelectronics technology. 35,36 In this work we synthesized GO from monolayer graphene by a downstream oxygen plasma. 37,38 The plasma-assisted technique has the advantages of short treatment time, an environmentally friendly manufacturing process, and the generation of numerous active species. 39 The immobilization of GOx en...

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Direct electrochemistry of glucose oxidase assembled on graphene and application to glucose detection

Cited by 236 publications

References 56 publications

Magnetic Fe3O4-Reduced Graphene Oxide Nanocomposites-Based Electrochemical Biosensing

Magnetic Fe3O4-Reduced Graphene Oxide Nanocomposites-Based Electrochemical Biosensing

Functionalized graphene oxide for clinical glucose biosensing in urine and serum samples

Functionalization of CVD Grown Graphene with Downstream Oxygen Plasma Treatment for Glucose Sensors

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