Rat thyroid graft transplantation after cryopreservation with scintigraphic standardization for an experimental study

Schanaider, Alberto; Barboza, Thiago; Vasconcellos, Marcel; Gutfilen-Schlesinger, Gabriel; Souza, Sérgio Augusto Lopes de

doi:10.1016/j.clinsp.2022.100065

Cited by 1 publication

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“…Studies were clustered according to their geographical distribution, half of which were published by US and European research groups [ 18 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 67 ] ( Figure 6 ), while the animals enrolled in the studies were mostly murine and canine [ 28 , 29 , 30 , 31 , 33 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 67 , 68 ], among which only one study discussed orthotopic autotransplantation into the remaining thyroid lobe [ 33 ]. In all the other concerned heterotopic autotransplantations, the preferred site was the muscle (posterior limb, neck, and dorsal-abdominal region) [ 18 , 32 , 36 , 37 , 38 , 41 , 42 , 43 , 44 , 68 ]. Autotransplantation has been immediate in more than half of the studies [ 18 , 27 , 29 , 30 , 31 , 32 , 33 , 34 , 37 ,…”

Section: Resultsmentioning

confidence: 99%

“…In all the other concerned heterotopic autotransplantations, the preferred site was the muscle (posterior limb, neck, and dorsal-abdominal region) [ 18 , 32 , 36 , 37 , 38 , 41 , 42 , 43 , 44 , 68 ]. Autotransplantation has been immediate in more than half of the studies [ 18 , 27 , 29 , 30 , 31 , 32 , 33 , 34 , 37 , 38 , 39 , 42 , 43 , 44 , 45 , 67 , 68 ], while post-cryopreservation autotransplantation entailed a minority of cases [ 27 , 36 , 38 , 41 , 44 , 68 ]. The follow-up of these studies lasted on average two months (range 0.25–12 months) and the animals were monitored at different intervals following surgery, assessing levels of T3, fT3, T4, fT4, TSH, scintigraphy and histological examination.…”

Section: Resultsmentioning

confidence: 99%

“…In 2021, Vasconcellos et al performed a study on post-cryopreservation thyroid autotransplantation in rats, because they have similar thyroid anatomy, histology and physiology to those of humans, suggesting to investigate the relationship between the duration of cryopreservation and the results of the autograft [ 41 ]. In 2022, Schanaider et al performed a successful study on post-cryopreservation thyroid autotransplantation in rats using Scintigraphy with 99mTcO4 to evaluate the functionality of thyroid grafts [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

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Cryopreserved Thyroid Tissue Autotransplant in Pediatric Age Patients: A Feasibility Study and Literature Review

Spinelli,

Ghionzoli,

Sahli

et al. 2024

Cancers

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Background and aims: This paper aims to study an alternative solution to hormonal replacement therapy in specific groups of patients who underwent thyroidectomy during childhood or adulthood. After cryopreservation, thyroid autotransplantation could be an alternative solution which would allow us to use the ability of the thyroid tissue of producing hormones according to the physiological needs of the body. Materials and methods: A feasibility study about the effects of the most modern cryopreservation techniques on the structural and functional integrity of the follicular cells of the thyroid tissue has been carried out. Patients who could benefit from the treatment have been found for both autotransplant techniques. Additionally, a literature review has been conducted. Results: The histological analysis has shown that cryopreservation does not alter the original architecture, and the culture examination that cell viability is successfully preserved. Moreover, both thyroid autotransplantation studies on animals and those on humans that were found in the literature have shown good results regarding the viability and functionality of the transplant. Conclusions: The viability of cryopreserved thyroid tissue found in this study is encouraging. Further studies to evaluate the levels of FT3, FT4 and thyroglobulin in thyroid tissue after cryopreservation are needed to verify that the secretory properties of the thyrocytes have been maintained intact. Furthermore, autotransplanted cases found in the literature do not have a long-term follow-up.

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