Acute exposure to boron in Nile tilapia (Oreochromis niloticus): Median-lethal concentration (LC50), blood parameters, DNA fragmentation of blood and sperm cells

Acar, Ümit; İnanan, Burak Evren; Zemheri, Fahriye; Kesbiç, Osman Sabri; Yılmaz, Sevdan

doi:10.1016/j.chemosphere.2018.09.063

Cited by 25 publications

(9 citation statements)

References 51 publications

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“…We observed increases in blood glucose level in the fish exposed to high temperature, possibly resulting from glycogenolysis to facilitate increased energy demands imposed by stressful conditions. Blood glucose level significantly increased under stress in Nile tilapia (Acar, Inanan, Zemheri, Kesbic, & Yılmaz, 2018) supported our findings. Cazenave et al.…”

Section: Discussionsupporting

confidence: 86%

Elevated temperature affects growth and hemato‐biochemical parameters, inducing morphological abnormalities of erythrocytes in Nile tilapiaOreochromis niloticus

et al. 2020

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Temperature is considered as an important environmental factor, and the increasing water temperature resulting from global warming is a great concern. The present study was conducted to examine the effects of elevated water temperature on growth, hemato‐biochemical parameters in Nile tilapia, Oreochromis niloticus acclimatized to three temperatures (31°C, 34°C and 37°C) for 60 days. Additionally, erythrocytic cellular abnormalities (ECA) and erythrocytic nuclear abnormalities (ENA) tests were assayed using peripheral erythrocytes after exposure to the three temperatures. Fish were sacrificed on days 7, 15, 30 and 60 of exposure. Growth performances viz., weight gain, % weight gain and specific growth rate (SGR) showed decreasing tendency at 34°C but significantly declined at 37°C compared to 31°C. The abundance of haemoglobin (Hb) and red blood cells (RBCs) significantly decreased in response to temperature increases, while white blood cells (WBCs) displayed the opposite response. At days 7 and 15, blood glucose levels significantly increased in response to the temperature increase, while at days 30 and 60 glucose declined. Frequencies of ECA and ENA were significantly enhanced at the highest temperature throughout the experimental period. Dissolved oxygen decreased and free CO2 increased significantly with increasing temperature throughout the study period. The present study revealed that temperatures higher than 34°C may be hazardous to O. niloticus.

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Section: Discussionsupporting

confidence: 86%

Elevated temperature affects growth and hemato‐biochemical parameters, inducing morphological abnormalities of erythrocytes in Nile tilapiaOreochromis niloticus

et al. 2020

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“…Her lamdan rastgele en az 25 hücre sayıldı ve her görüntü kuyruklu yıldız kuyruğundaki floresan yoğunluğuna göre sınıflandırıldı. Skorlamada 0, 1, 2, 3 veya 4 (Skor 0: hasar görmemiş, Skor 4: maksimum hasar görmüş) değerleri kullanıldı [20,21]. [22].…”

Section: Genotoksisite (Dna Hasarı) çAlışmalarıunclassified

MCF-7 ve PC-3 Hücre Hatlarında, Hücre Canlılığı ve DNA Hasarı Üzerine TRPV4 Antagonisti RN 1734'ün Etkileri

Çakır¹,

Erden²

2019

Bozok Tıp Dergisi

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Hücre içi kalsiyum (Ca 2 +) sinyali; hücre proliferasyonu ve farklılaşması, gen transkripsiyonu, apoptozis gibi birçok hücresel süreçte rol oynar. Yapılan çalışmalarda Ca 2 + sinyalinin kanser ile ilişkili olduğu gösterilmiştir. Transient receptor potential vanilloid 4 (TRPV4) kanalları hücre zarında bulunan Ca 2 + geçirgen non-selekif katyon kanalıdır. TRPV4 kanalları vücutta birçok dokuda yaygın olarak eksprese edilmektedir. Hücre içi Ca 2 + miktarının düzenlenmesinde etkisi olan TRPV4 kanallarının kanser hücrelerinin proliferasyonu, apoptozis, migrasyonu ve tümor anjiogenezi ile ilgili önemli rolleri olduğu görülmüştür. Biz bu çalışmada TRPV4 antagonisti olan RN 1734'ün, insan prostat (PC-3) ve insan meme (MCF-7) kanseri hücre hatlarında hücre canlılığı ve DNA hasarı üzerine etkilerini araştırdık. Gereç ve Yöntemler: Çalışmamızda insan prostat (PC-3) ve insan meme (MCF-7) kanseri hücre hatları kullanıldı. RN 1734 1, 5, 25, 50 ve 100 μM'lik konsantrasyonlarda, 24 saatlik PC-3 ve MCF-7 hücre canlılığına olan etkisi 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromid (MTT) yöntemiyle ölçüldü. RN 1734'ün DNA hasarına etkisi Comet yöntemine göre belirlendi. Bulgular: 24 saat süreyle RN 1734'ün özellikle 50 ve 100 μM'lik konsantrasyonlarıyla inkübe edilen PC-3 ve MCF-7 hücrelerde canlılık düzeyi önemli ölçüde azaldı (p<0.05). Ayrıca 100 μM'lik konsantrasyonda RN 1734 uygulaması sonrası hücre DNA hasarı düzeyinde anlamlı artışın olduğu görüldü (p<0.05). Sonuç: Biz bu çalışmada TRPV4 antagonisti olan RN 1734'ün, PC-3 ve MCF-7 hücre hatlarında hücre canlılığını azalttığını ve DNA hasarına neden olduğunu bulduk. Bizim bulgularımız, TRPV4 antagonisti uygulanmasının kanser hücrelerine karşı yeni bir tedavi yaklaşımı olabileceğini düşündürmektedir.

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“…Generally, the analytical grade imidacloprid showed no toxicity effects on the development of Zebrafish embryos even at 320 mg L −1 , on the contrary, the mortality rate of adult zebrafish ( Danio rerio ) exposed to the commercial formulation Confidor SL 200 was more influenced than the developmental larvae (Tišler et al, 2009). Also, the value of 96 h LC50 was 550 mg L −1 for L. rohita (Qadir et al, 2014) and 141.42 mg L −1 for Tilapia (Acar et al, 2018) treated with Imidacloprid. The median inhibitory concentrations of imidacloprid commercial product were informed in vitro in flounder ( Paralichthys olivaceus ), that is, 38.5 to 41.9 mg L −1 (Su et al, 2007), whereas other species such as rainbow trout ( Oncorhynchus mykiss ) and common carp ( Cyprinus carpio ) had 211 and 280 mg L −1 96‐h LC50 levels respectively (Tišler et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

The new aspects of using some safe feed additives on alleviated imidacloprid toxicity in farmed fish: a review

Naiel

Shehata

Negm

et al. 2020

Reviews in Aquaculture

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Intensive fish culturing systems depend on provides optimal water quality conditions. Hence, the most important aquaculture practices are the removal of contamination elements related not only to the water quality parameters unacceptable for aquatic species but also to the undesirable effect on the health of fish and consumers. During the last three decades, common applications and production of pesticides have amplified progressively. Due to the excessive use of imidacloprid as a highly effective insecticide, elevated accumulation is expected in an aquatic environment. A large part of applied pesticides emitted into the environment and only a small portion of them reach the final biological target, where it caused several hazards, such as accumulation and toxicity to non‐target creatures. Plant‐derived secondary metabolites are getting great attention due to their safety and therapeutic functions. Also, several feed additives in animal diets can act as chemopreventive agents, which have the potential to decrease the toxicity risk of many pollutants and detoxification of activated metabolites. Understanding the internal action mechanisms of various safe feed additives could lead to the novel therapeutic approaches for acute and chronic pesticide toxicity. This review intends to summarize how safe feed additives alter or mitigate the toxic effects of imidacloprid in farmed fish.

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Acute exposure to boron in Nile tilapia (Oreochromis niloticus): Median-lethal concentration (LC50), blood parameters, DNA fragmentation of blood and sperm cells

Cited by 25 publications

References 51 publications

Elevated temperature affects growth and hemato‐biochemical parameters, inducing morphological abnormalities of erythrocytes in Nile tilapiaOreochromis niloticus

Elevated temperature affects growth and hemato‐biochemical parameters, inducing morphological abnormalities of erythrocytes in Nile tilapiaOreochromis niloticus

MCF-7 ve PC-3 Hücre Hatlarında, Hücre Canlılığı ve DNA Hasarı Üzerine TRPV4 Antagonisti RN 1734'ün Etkileri

The new aspects of using some safe feed additives on alleviated imidacloprid toxicity in farmed fish: a review

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