Surveillance of bacteria Pseudomonas aeruginosa and MRSA associated with chronic suppurative otitis media

Rath, Sibanarayan; Das, Sharmistha; Padhy, Rabindra N.

doi:10.1016/j.bjorl.2016.03.008

Cited by 17 publications

(13 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The finding of this study regarding the P.aeruginosa predominance in CSOM was in agreement with local studies; Alsaimary, et al (19.4%)27Al-Hilli,201528, Aldhaher andHassan,2018(Aldhaher &Hassan, 2018;Arwa Hammodi Karim, 2005). Similarly, the finding of this study regarding the predominance of P.aeruginosa was also in agreement with other global studies (Chirwa et al, 2015;Madana et al, 2011;Rath, Das, & Padhy, 2017). The predominance of P.aeruginosa in CSOM can be accounted to their minimal nutritional requirement and potential ability to resist antibiotics (Pollock .M, 1996).…”

Section: Discussionsupporting

confidence: 92%

Molecular Characterization of Virulence and Antibiogram profile of Pseudomonas aeruginosa Isolated from Chronic Suppurative Otitis Media Patients, Al-Nasiriyah City, Iraq.

2021

University of Thi-Qar Journal of Science

View full text Add to dashboard Cite

This study was conducted to characterize the virulence of P.aeruginosa isolated from Chronic Suppurative Otitis Media (CSOM) in a particular area at the south of Iraq, Al-Nasiryah City. Conventional bacteriological methods were used to isolate and identify P.aeruginosa. Polymerase chain reaction (PCR) with a specific primer that targeting outer membrane Liporpotien L gene (OprL) was used to detect P.aeruginosa. The following virulence genes were screened; ToxA, LasB and LasA. Results indicated that the majority of CSOM was attributed to bacterial infection(83.4%) in comparison with only 2(5.6%) were fungal infections. P.aeruginosa was the most predominant species (36.7%) followed by S.aurues, Proteus Spp. and E.coli. Virulence profile revealed that all strains of P.aeruginosa were harboring at least one virulence gene. ToxA gene was found in most strains (90.1%) followed by LasB gene (81.8%) and LasA gene (63.6%). According to different combination of virulence genes, P.aeruginosa can be categorized into four genotypes with high prevalence (63.6%) of the most virulent genotype (ToxA+ LasA+ Las B+) followed by genotype (ToxA+ LasA-Las B+) (18.6%) while the genotypes (ToxA+ LasA-LasB and ToxA+ LasA-LasB-) were found in lowest percentage (9.1%). Additionally, all strains were able to form a biofilm with varying propensity however, the strong biofilm strains were found in a high percentage (72.7%) in genotype (ToxA+ LasA+ Las B+). Antibiogram analysis revealed that high resistance rate against otototic antibiotics including; Erythromycine, Ciprofloxacin(63%), Gentamycin, Ofloxacin and Ceftriaxone were(47.4%, 47.3% and 42.1% respectively). In conclusions, the P.aeruginosa causing CSOM in Al-Nasiriyah city is highly virulent and have a great ability to form biofilm, which could protect it from most commonly used antibiotics especially those used in Al-Nasiriyah, so it is highly recommended to seek for alternative treatment.

show abstract

Section: Discussionsupporting

confidence: 92%

Molecular Characterization of Virulence and Antibiogram profile of Pseudomonas aeruginosa Isolated from Chronic Suppurative Otitis Media Patients, Al-Nasiriyah City, Iraq.

2021

University of Thi-Qar Journal of Science

View full text Add to dashboard Cite

show abstract

“…As a pathogen with extremely high prevalence, S. aureus causes various clinical infections such as skin and soft tissue infections (SSTIs) [19], invasive community-acquired MRSA (CA-MRSA) infections [21], and pneumonia [22]. Few studies about MRSA and MSSA isolates contemporaneously circulating among age-specific groups of children attending otolaryngology clinics have been examined [23, 24]. According to the previous report [11], hospital-acquired MRSA (HA-MRSA) is usually detected with SCC mec type I, II and III, while CA-MRSA is usually detected with SCC mec type IV, IVa and V. In this study, all of the MRSA isolates carried SCC mec IV, IVa and V, which confirmed that these MRSA isolates belonged to CA-MRSA.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and antimicrobial susceptibility of Staphylococcus aureus isolated from children with acute otitis media in Liuzhou, China

Ding

Chen

et al. 2018

BMC Pediatr

View full text Add to dashboard Cite

BackgroundThere have been few studies focused on the prevalence, bacterial etiology, antibiotic resistance, and genetic background of Staphylococcus aureus (S. aureus) in children with acute otitis media (AOM) in China.MethodsA retrospective study was conducted in Liuzhou Maternity and Child Healthcare Hospital. Patients younger than 18 years diagnosed with AOM were enrolled in the study. Middle ear fluid specimens were collected and cultured for bacterial pathogens. The antibiotic susceptibility, virulence genes, macrolide resistant genes and sequence types of S. aureus were identified.ResultsFrom January 1, 2013 to December 31, 2015, a total of 228 cases of AOM were identified. Pathogenic bacteria were positive in 181 (79.4%) of 228 specimens. Streptococcus pneumoniae was the most common bacteria (36.4%), followed by S. aureus (16.2%). Among the 37 S. aureus isolates, 12 (23.5%) were methicillin-resistant S. aureus (MRSA), and 25 (77.5%) were methicillin-susceptible S. aureus (MSSA). A total of 23 isolates (62.2%) were resistant to erythromycin, 40.5% of isolates were resistant to clindamycin, and 37.8% isolates were resistant to tetracycline. Twenty-three isolates were multi-drug resistant (MDR) S. aureus. Eighteen isolates carried the pvl gene. Up to 22 (59.4%) isolates expressed ermA gene, 8 (21.6%) isolates expressed both ermA and ermC genes, and only 8.1% expressed ermB. Among all S.aureus isolates, 7 sequence types (STs) were identified by multilocus sequence typing (MLST). The most common ST was ST59 (16/37, 43.2%), followed by ST45 (7/37, 18.9%) and ST30 (7/37, 18.9%). The predominant MSSA isolates were ST59-t437-MSSA (5/25, 20.0%), the prevailing MRSA isolates were Taiwan related strains ST59-SCCmec-IVa/V (5/12, 41.6%).ConclusionsS. aureus was the second most common cause for AOM in children in Liuzhou. Most of the S. aureus was MDR which carried a high proportion of ermA and ermC gene. CA-MRSA (ST59-SCCmec-IV/V-t437) is circulating in children with AOM. These findings support continued surveillance of S. aureus infections in children with AOM in both communities and hospitals.

show abstract

“…Both, bacterial cells; Gram-negative MDR-PA and Gram-positive MRSA, and C. albicans fungi are well known for their biofilm producing ability and chronic nosocomial infections spread in hospital and associated settings [35,36]. Although, several metallic nanoantibiotics were found having great potential either to cease or eradicate biofilm adherence [37].…”

Section: Antibiofilm Studies Of Liv-agnpsmentioning

confidence: 99%

Biofabricated Fatty Acids-Capped Silver Nanoparticles as Potential Antibacterial, Antifungal, Antibiofilm and Anticancer Agents

et al. 2021

View full text Add to dashboard Cite

The current study demonstrates the synthesis of fatty acids (FAs) capped silver nanoparticles (AgNPs) using aqueous poly-herbal drug Liv52 extract (PLE) as a reducing, dispersing and stabilizing agent. The NPs were characterized by various techniques and used to investigate their potent antibacterial, antibiofilm, antifungal and anticancer activities. GC-MS analysis of PLE shows a total of 37 peaks for a variety of bio-actives compounds. Amongst them, n-hexadecanoic acid (21.95%), linoleic acid (20.45%), oleic acid (18.01%) and stearic acid (13.99%) were found predominately and most likely acted as reducing, stabilizing and encapsulation FAs in LIV-AgNPs formation. FTIR analysis of LIV-AgNPs shows some other functional bio-actives like proteins, sugars and alkenes in the soft PLE corona. The zone of inhibition was 10.0 ± 2.2–18.5 ± 1.0 mm, 10.5 ± 2.5–22.5 ± 1.5 mm and 13.7 ± 1.0–16.5 ± 1.2 against P. aeruginosa, S. aureus and C. albicans, respectively. LIV-AgNPs inhibit biofilm formation in a dose-dependent manner i.e., 54.4% ± 3.1%—10.12% ± 2.3% (S. aureus), 72.7% ± 2.2%–23.3% ± 5.2% (P. aeruginosa) and 85.4% ± 3.3%–25.6% ± 2.2% (C. albicans), and SEM analysis of treated planktonic cells and their biofilm biomass validated the fitness of LIV-AgNPs in future nanoantibiotics. In addition, as prepared FAs rich PLE capped AgNPs have also exhibited significant (p < 0.05 *) antiproliferative activity against cultured HCT-116 cells. Overall, this is a very first demonstration on employment of FAs rich PLE for the synthesis of highly dispersible, stable and uniform sized AgNPs and their antibacterial, antifungal, antibiofilm and anticancer efficacy.

show abstract

Surveillance of bacteria Pseudomonas aeruginosa and MRSA associated with chronic suppurative otitis media

Abstract: Multidrug resistant strains of P. aeruginosa were more prevalent than those of S. aureus in ear discharges. Tobramycin and cloxacillin may be included in the formulatory antibiotic regimen to overcome bacterial infections in chronic suppurative otitis media.

Cited by 17 publications

References 15 publications

Molecular Characterization of Virulence and Antibiogram profile of Pseudomonas aeruginosa Isolated from Chronic Suppurative Otitis Media Patients, Al-Nasiriyah City, Iraq.

Molecular Characterization of Virulence and Antibiogram profile of Pseudomonas aeruginosa Isolated from Chronic Suppurative Otitis Media Patients, Al-Nasiriyah City, Iraq.

Molecular characterization and antimicrobial susceptibility of Staphylococcus aureus isolated from children with acute otitis media in Liuzhou, China

Biofabricated Fatty Acids-Capped Silver Nanoparticles as Potential Antibacterial, Antifungal, Antibiofilm and Anticancer Agents

Contact Info

Product

Resources

About