Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana

Abstract

Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition. However, while the bacterial community was rather stable, pronounced shifts were observed in the abundance of bacteria carrying plasmids. A PCR-Southern blot hybridization analysis indicated an increase in the abundance of IncP-9 catabolic plasmids. Denaturing gradient gel electrophoresis of naphthalene dioxygenase (ndo) genes amplified from cDNA (RNA) indicated the dominance of a specific ndo gene in the inoculated petroleum amendment treatment. The petroleum hydrocarbon degrading consortium characterization indicated the prevalence of bacteria assigned to Pseudomonas spp., Comamonas spp. and Ochrobactrum spp. IncP-9 plasmids were detected for the first time in Comamonas sp. and Ochrobactrum spp., which is a novelty of this study.

Synergistic PAHs biodegradation by a mixed bacterial community: Based on a multisubstrate enrichment approach

“…Ralstonia (Di Gioia et al, 2002) and Achromobacter (Pradeep et al, 2015) have been reported as showing the ability of degrading catechol and phthalic acid, respectively, and Delftia have not previously been reported with biodegradation of salicylic acid. The contribution of the other genera with increasing percentages associated with PAHs biodegradation are supported by previous studies for Stenotrophomonas (Zang et al, 2020), Pseudacidovorax (Dealtry et al, 2018), Novosphingobium (Fida et al, 2017), Enterobacter (Lors et al, 2012), Comamonas (Qin et al, 2019), Chryseobacterium (Xiao et al, 2019), and Burkholderia (Morya et al, 2020). After the acclimation that phenanthrene and pyrene were added as carbon source, the dominant genus was Pseudomonas again in sample MBF.…”

Isolation and Identification of Hydrocarbon-Degrading Bacteria that Tolerant to Saponin of Sapindus Rarak Plant

“…Plasmid incP-9 plays to start the process of degradation of oil. Other research explain that the IncP-9 plasmids detected in the Genus of Ochrobactrum and Pseudomonas (Dealtry et al, 2018). From the results of this research obtained three isolates belonging to the Genus of Ochrobactrum and Pseudomonas.…”

Effect of green synthesized iron oxide nanoparticles on bacterial microbiome for clean up the crude oil

“…The total petroleum hydrocarbons content decreased within consortia inoculating with the three species by 18.3 (mg g −1 ), 83 % of crude oil residues (1%, v/v) during 22 days of treatment (Tanzadeh et al, 2020). Dealtry et al (2018) scanned the bacterial community of petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana for decontamination of artificial petroleum medium. They considered the bacterial 16S rRNA gene partial sequence and analyzed for the determination of incompatible plasmids as well as naphthalene dioxygenase genes via dot blot hybridization.…”

“…They considered the bacterial 16S rRNA gene partial sequence and analyzed for the determination of incompatible plasmids as well as naphthalene dioxygenase genes via dot blot hybridization. They expounded the prevalence of Bacillus infantis after 10 days on incubation, with other bacterial species assigned to Pseudomonas sp., Ochrobactrum sp, and etc (Dealtry et al, 2018). Joe et al ( 2019) (Joe et al, 2019) isolated, identified a rhamnolipidproducing bacterial species (e.g., Shewanella sp.…”