Streptomyces globosus DK15 and Streptomyces ederensis ST13 as new producers of factumycin and tetrangomycin antibiotics

Abstract

Fifty seven soil-borne actinomycete strains were assessed for the antibiotic production. Two of the most active isolates, designed as Streptomyces ST-13 and DK-15 exhibited a broad range of antimicrobial activity and therefore they were selected for HPLC fractionation against the most suppressed bacteria Staphylococcus aureus (ST-13) and Chromobacterium violaceum (DK-15). LC/MS analysis of extracts showed the presence of polyketides factumycin (DK15) and tetrangomycin (ST13). The taxonomic position of the antibiotic-producing actinomycetes was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolates matched those described for members of the genus Streptomyces. DK-15 strain exhibited the highest 16S rRNA gene sequence similarity to Streptomyces globosus DSM-40815 (T) and Streptomyces toxytricini DSM-40178 (T) and ST-13 strain to Streptomyces ederensis DSM-40741 (T) and Streptomyces phaeochromogenes DSM-40073 (T). For the proper identification, MALDI-TOF/MS profile of whole-cell proteins led to the identification of S. globosus DK-15 (accession number: KX527570) and S. ederensis ST13 (accession number: KX527568). To our knowledge, there is no report about the production of these antibiotics by S.globosus and S. ederensis, thus isolates DK15 and ST13 identified as S. globosus DK-15 and S.ederensis ST-13 can be considered as new sources of these unique antibacterial metabolites.

A Study on actinobacterial diversity of Hampoeil cave and screening of their biological activities

“…The seeding material was prepared by inoculation of the isolates into ISP2 broth and incubated at 180 rpm, 28 °C, 48 h. The seeding material (10% v/v) was transferred into the 1000 ml Erlenmeyer containing 250 ml of fermentation medium and the fermentation flasks were incubated at 28 °C for 7 days, 180 rpm. After removing the biomass by centrifugation of the fermentation broth at 2500 g for 10 min, the antimicrobial activity of the supernatant were assayed against several test strain microorganisms including, Escherichia coli UTMC 1465, E. coli TolC UTMC 1462, Pseudomonas aeruginosa UTMC 1463, Micrococcus luteus UTMC 1461, Mucor hiemalis UTMC 5057, Chromobacterium violaceum UTMC 1466, Candida albicans UTMC 5055, Staphylococcus aureus UTMC 1467, Pichia anomala UTMC 5056, and Bacillus subtilis UTMC 1464 by well agar diffusion method (Charousová et al, 2018). All test strains were obtained from University of Tehran Microorganisms Collection.…”

A Novel Insight Into the Fabrication of Polyhydroxyalkanoates from Actinobacteria Streptomyces toxytricini D2: Screening, Optimization, and Biopolymer Characterization

“…The percentage of inoculum applied on the microbiological fermentation process received more attention as it determine the volume of yield and time duration of production process (Charousová et al, 2018). In this study, about 8% of inoculum of S. toxytricini D2 produced a reasonable quantity (66.7%) of PHAs (12.34 g L − 1 of PHAs from 18.5 g L − 1 of cell biomass) in a short duration of incubation than other dosages (Fig.…”

A novel insight into the fabrication of Polyhydroxyalkanoates from actinobacteria Streptomyces toxytricini D2: screening, optimization, and biopolymer characterization

“…2). The results were directly compared with the previous ndings (Charousová et al, 2018;Salgaonkar & Bragança, 2017). They isolated commercially valuable Streptomyces sp.…”

“…The favorable growth conditions are the most signi cant factor in attaining the expression of the maximum potential of all organisms (Charousová et al, 2018). Similarly, the bacteria also required the most suitable growth conditions for more human and environmental welfare products such as PHA (Krishnan et al, 2017).…”