2018
DOI: 10.1016/j.bjm.2017.04.009
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Q fever is a worldwide zoonosis caused by Coxiella burnetii—a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specificity of testing while offering a suitable amplicon size for sequencing. Serial dilutions were performed tenfold to te… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

0
8
0

Year Published

2018
2018
2023
2023

Publication Types

Select...
10

Relationship

2
8

Authors

Journals

citations
Cited by 11 publications
(8 citation statements)
references
References 24 publications
0
8
0
Order By: Relevance
“…For amplification, the reaction contained 1X PCR buffer 10 X, 0.2 μM of each primer (Invitrogen, Life Technologies Brazil), 1.5 mM MgCl 2 , 200 μM dNTP mixture (20 mM of each deoxynucleotide triphosphate), 0.5 U Platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA), 2 μL of sample DNA, and nuclease-free water (Promega, Madison, WI, USA) in a volume of 25 μL. The amplification was performed in a thermocycler (Applied Biosystem Veriti 96) using the previously described cycling conditions [ 29 ]. To confirm the amplification, the generated products were separated on a 1% agarose gel stained with 10 μL of RED Gel solution (10,000X) per 100 μL of agarose gel.…”
Section: Methodsmentioning
confidence: 99%
“…For amplification, the reaction contained 1X PCR buffer 10 X, 0.2 μM of each primer (Invitrogen, Life Technologies Brazil), 1.5 mM MgCl 2 , 200 μM dNTP mixture (20 mM of each deoxynucleotide triphosphate), 0.5 U Platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA), 2 μL of sample DNA, and nuclease-free water (Promega, Madison, WI, USA) in a volume of 25 μL. The amplification was performed in a thermocycler (Applied Biosystem Veriti 96) using the previously described cycling conditions [ 29 ]. To confirm the amplification, the generated products were separated on a 1% agarose gel stained with 10 μL of RED Gel solution (10,000X) per 100 μL of agarose gel.…”
Section: Methodsmentioning
confidence: 99%
“…The reaction mixture contained 5 μL of DNA, 10 μL of 2× Platinum™ SuperFi™ PCR Master Mix (Invitrogen, Carlsbad, CA), 2.0 μL of Is1Pri_f (10 pmol), 2.0 μL of primer Is1Pri_r (10 pmol), 0.3 μL of probe Tqpro_IS1, and water sufficient to make up the reaction volume. The PCR thermal profile used for optimized conventional PCR was applied, and reactions were carried out using a 7500 Fast Real-Time PCR system (Applied Biosystems, USA) [ 31 ].…”
Section: Methodsmentioning
confidence: 99%
“…Such a study was done in the Netherlands [ 19 ] after the largest reported Q-fever outbreak which occurred during 2007–2010 and was linked to dairy goat farms near densely populated areas in the southeast of the country. Q-fever, caused by the intracellular coccobacillus Coxiella burnetii , is a zoonosis (a disease caused by germs that are transmitted from animals to humans) that occurs worldwide [ 20 , 21 ]. It is presumed to have involved human exposure via a wind-borne route [ 21 ] and resulted in 4223 notifications of Q-fever [ 22 ].…”
Section: Introductionmentioning
confidence: 99%