Temporal assessment of microbial communities in soils of two contrasting mangroves

Abstract

Variations in microbial communities promoted by alterations in environmental conditions are reflected in similarities/differences both at taxonomic and functional levels. Here we used a natural gradient within mangroves from seashore to upland, to contrast the natural variability in bacteria, cyanobacteria and diazotroph assemblages in a pristine area compared to an oil polluted area along a timespan of three years, based on ARISA (bacteria and cyanobacteria) and nifH T-RFLP (diazotrophs) fingerprinting. The data presented herein indicated that changes in all the communities evaluated were mainly driven by the temporal effect in the contaminated area, while local effects were dominant on the pristine mangrove. A positive correlation of community structure between diazotrophs and cyanobacteria was observed, suggesting the functional importance of this phylum as nitrogen fixers in mangroves soils. Different ecological patterns explained the microbial behavior in the pristine and polluted mangroves. Stochastic models in the pristine mangrove indicate that there is not a specific environmental factor that determines the bacterial distribution, while cyanobacteria and diazotrophs better fitted in deterministic model in the same area. For the contaminated mangrove site, deterministic models better represented the variations in the communities, suggesting that the presence of oil might change the microbial ecological structures over time. Mangroves represent a unique environment threatened by global change, and this study contributed to the knowledge of the microbial distribution in such areas and its response on persistent contamination historic events.

Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka

“…Tembelok mangroves include mangroves with minimal human activity around them, while the coastal areas around the mangrove Sukal are used for cultivation of blood clams and Peltim Mangroves adjacent to the tin smelting industry. Rigonato (2017) shows that pristine mangroves have a pronounced effect on microbial community composition and contaminated mangroves that have different microbial communities at different times. Chemical pollution, particularly accumulation and bio-transformation of toxic metals could be a significant factor for reduction of mangrove biodiversity (Maiti and Chowdhury, 2013).…”

Técnicas aplicadas en la investigación agrícola para cuantificar la fijación de nitrógeno: una revisión sistemática

“…Los genes nifH se encuentran diversificados y su transcripción está controlada por la ARN polimerasa, que es una holoenzima alternativa, y también necesita una proteína activadora nifA (Satyanarayana, Krishna, Pavan, & Jeereddy, 2018). Otros genes identificados por PCR son 16S rRNA (Akemi et al, 2017;Jaiswal, Msimbira, & Dakora, 2017;Tahon & Willems, 2017), porque permite una clasificación filogenética más detallada a nivel de especie (Osei, Abaidoo, Ahiabor, & Boddey, 2018;Ribeiro et al, 2018), genes rec A, atpD, gln II y nod C con análisis de secuencia multilocus (MLSA) (Leite et al, 2017;Mwenda, Hara, De Meyer, Howieson, & Terpolilli, 2018;Rigonato et al, 2017). Con esta técnica, se ha logrado identificar el gen nifH en géneros Paenibacillus, Enterobacter, Klebsiella y Pantoea (Ndungu et al, 2018), que no son reconocidos por fijar nitrógeno, de modo que es posible que hayan obtenido la capacidad de fijación de nitrógeno a través de la transferencia lateral de genes; sin embargo, las fuerzas evolutivas de la transferencia lateral de genes no son bien conocidas (Khalifa & AlMalki, 2018).…”

Identifikasi Molekuler Isolat Bakteri Selulolitik Dari Mangrove Sungailiat Dan Tukak Sadai Di Pulau Bangka

“…Mangrove adalah ekosistem unik yang bermanfaat bagi ekosistem seperti perlindungan bahaya tsunami, habitat organisme muara dan penyuplay oksigen. Mangrove adalah ekosistem dengan kepentingan ekologis yang besar di daerah pesisir, di mana mereka menyediakan sumber makanan dan tempat hidup benih bagi banyak hewan laut dan spesies laut yang disesuaikan dengan lingkungan payau / garam (Rigonato et al, 2017). Mikroba pada mangrove memainkan peran penting dalam siklus siklus karbon organik.…”