Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain

Abstract

Bacillus anthracis strain SPV842_15 was isolated from bovine fetus, while B. anthracis strain Brazilian vaccinal was recovered from a commercial vaccine. We report here the genome sequences of both strains. The SPV842_15 genome is composed of a single circular chromosome with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn, the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Both genomes have an overall C + G content of 35.4%, and 11 genes encoding the ribosomal RNAs (rRNAs) 5S, 16S and 23S. Only the plasmid pX01 sequence, which carries genes for toxins synthesis, was detected and completely assembled for both strains. These plasmids have a length of 181,684 base pairs and a C + G content of 32.5%. These genomic data generate insights about vaccinal B. anthracis virulence.

Bovine abortion by a vaccine strain of Bacillus anthracis

“…The complete genome and pX01 plasmid sequences of B. anthracis strain SPV842_15 have the accession numbers CP019588.1 and CP019589.1, respectively. The B. anthracis Brazilian vaccinal strain genome (as the shotgun genome) has the accession MVOA00000000 and its pX01 plasmid under number CM007716.1 (SIQUEIRA et al, 2017).…”