Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen

Song, Yun-Hee; Lee, Kyung-Tai; Baek, Jin-Young; Kim, Min-Ju; Kwon, Mi-Ra; Kim, Young-Joo; Park, Mi-Rim; Ko, Haesu; Lee, Jin Sung; Kim, Keun-Sung

doi:10.1016/j.bjm.2017.03.006

Cited by 22 publications

(11 citation statements)

References 35 publications

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“…Similarly, there are reports for the recombinant EG with a pH range of 4.0 to 9.0 and a temperature range of 20–70°C ( Furtado et al, 2011 ). The rumen metagenome-derived EG showed a pH range of 4.0–10.0 and a temperature range of 20–70°C ( Song et al, 2017 ). The EG from Thermotoga maritima showed a pH range of 4.0–8.0 (optimum pH 5.0) and a temperature range of 30–100°C (optimum temperature 95°C; Wang et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

A thermo-alkali stable and detergent compatible processive β-1,4-glucanase from Himalayan Bacillus sp. PCH94

Thakur

Singh

2022

Front. Microbiol.

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Present study reports a novel and robust GH9 processive endoglucanase β-1,4-glucanase from Bacillus sp. PCH94 (EGaseBL) with thermo-alkali stable properties. The EGaseBL gene was cloned in pET-28b(+) and expressed in Escherichia coli BL21(DE3) cells. The recombinant protein was purified 94-fold with a yield of 67.8%. The biochemical characterization revealed an active enzyme at a wide pH (4.0–10.0) and temperature (4–100°C). It showed a Km and Vmax of 1.10 mg/ml and 208.24 IU/mg, respectively, using β-glucan as a substrate. The EGaseBL showed dual activities for endoglucanase (134.17 IU/mg) and exoglucanase (28.76 IU/mg), assayed using substrates β-glucan and Avicel, respectively. The enzyme is highly stable in neutral and alkaline pH and showed a half-life of 11.29 h, and 8.31 h in pH 7.0 and 9.0, respectively. The enzyme is also compatible with commercial detergents (Tide, Surf, Ghadi, Raj, and Healing tree) of the Indian market and retained > 85% enzyme activity. Concisely, robustness, extreme functionality, and detergent compatibility endorse EGaseBL as a potential bioresource for the detergent industry, in addition to its implications for the bioethanol industry.Highlights– Cloning, expression, and purification of putative novel GH9 family β-1,4-glucanase.– Processive endoglucanase with CBM3 domain and bi-functional (endo/exo) activity.– Broad pH-temperature active and stable enzyme.– Compatible with commercial detergent powders.

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Section: Resultsmentioning

confidence: 99%

A thermo-alkali stable and detergent compatible processive β-1,4-glucanase from Himalayan Bacillus sp. PCH94

Thakur

Singh

2022

Front. Microbiol.

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“…Song et al . [ 31 ] reported that the cellulases from rumen bacteria retain 70% of its activity in the pH range from 5 to 7 and in a temperature range from 30°C to 50°C. pH of ruminants ranges from 5 to 7, under normal dietary conditions and the ruminal temperature can vary from 38°C to 41°C.…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of a cellulolytic Enterobacter from rumen of Aceh cattle

Sari¹,

Safika²,

Darmawi³

et al. 2017

Vet World

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Aims:The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid of Aceh’s cattle. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria that act to degrade feed fibrous material so as to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzymes.Materials and Methods:Samples were collected from rumen fluid of Aceh’s cattle in Abattoirs (RPH) of Banda Aceh city, Indonesia, isolation, and screening of cellulolytic bacteria were done in Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.Results:The S1 isolates showed ±2.5 cm of clear zone diameter. Microscopically, this strain was found to be a Gram-negative, Bacillus. Homology and phylogenetic tree analysis of 16S rRNA showed that S1 isolate has 91% of sequence similarity with that of Enterobacter cloacae. 91% sequence homology shown in this study proved that the S1 isolate is probably either a new species or another genus of Enterobacteriaceae.Conclusion:Current study suggests that cellulose hydrolytic bacteria isolated from rumen fluid of Aceh cattle on Bushnell Haas medium-carboxymethylcellulose agar, and some potent cellulose degrading bacteria have been identified.

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“…For example, Song et al extracted high-molecular-weight DNA from the contents of the rumen and fragmented these DNAs to sizes ranging from 10 to 50 kbp before creating fosmid libraries. The clones were screened for cellulolytic activity, and those with positive cellulolytic activity were pooled for DNA extraction and sequencing ( 10 ). Further gene annotation revealed a novel glycosyl hydrolase family 5 cellulase gene with endo-β-1,4-glucanase.…”

Section: Functional Metagenomicsmentioning

confidence: 99%

Metagenomics as a Tool To Monitor Reclaimed-Water Quality

Hong

Mantilla‐Calderon

Wang

2020

Appl Environ Microbiol

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Many biological contaminants are disseminated through water, and their occurrence has potential detrimental impacts on public and environmental health. Conventional monitoring tools rely on cultivation and are not robust in addressing modern water quality concerns. This review proposes metagenomics as a means to provide a rapid, nontargeted assessment of biological contaminants in water. When further coupled with the appropriate methods (e.g., quantitative PCR and flow cytometry) and bioinformatic tools, metagenomics can provide information concerning both the abundance and diversity of biological contaminants in reclaimed waters. Further correlation between the metagenomic-derived data of selected contaminants and the measurable parameters of water quality can also aid in devising strategies to alleviate undesirable water quality. Here, we reviewed metagenomic approaches (i.e., both sequencing platforms and bioinformatic tools) and studies that demonstrated their use for reclaimed water quality monitoring. We also provide recommendations on areas of improvement that will allow metagenomics to significantly impact how the water industry performs reclaimed water quality monitoring in the future.

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Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen

Cited by 22 publications

References 35 publications

A thermo-alkali stable and detergent compatible processive β-1,4-glucanase from Himalayan Bacillus sp. PCH94

A thermo-alkali stable and detergent compatible processive β-1,4-glucanase from Himalayan Bacillus sp. PCH94

Isolation and identification of a cellulolytic Enterobacter from rumen of Aceh cattle

Metagenomics as a Tool To Monitor Reclaimed-Water Quality

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