Antimicrobial activity evaluation and comparison of methods of susceptibility for Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacter spp. isolates

Rechenchoski, Daniele Zendrini; Dambrozio, Angélica Marim Lopes; Vivan, Ana Carolina Polano; Schuroff, Paulo Alfonso; Burgos, Tatiane das Neves; Pelisson, Marsileni; Perugini, Marcia Regina Eches; Vespero, Eliana Carolina

doi:10.1016/j.bjm.2017.01.008

Cited by 27 publications

(18 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A study has reported that the sensitivity profile of microorganisms to antimicrobials can range from 0% to 4% when tested on Klebsiella pneumoniae (24) . Similar data were also found in the Serratia marcescens and Enterobacter cloacae species (25)(26) . It is important to emphasize the fact that the therapeutic limitation is a problem due to the rapid dissemination of resistance genes among the community, negatively impacting the outcome of hospitalized patients (24) .…”

Section: Discussionsupporting

confidence: 83%

Epidemiological profile of healthcare-associated infections caused by Carbapenemase-producing Enterobacteriaceae

Alvim

Couto

Gazzinelli

2019

Rev. esc. enferm. USP

View full text Add to dashboard Cite

Objective: To study the epidemiological profile of Healthcare-associated Infections caused by Enterobacteria which carry the Klebsiella pneumoniae Carbapenemase gene (blaKPC) in the hospital environment. Method: A descriptive study was conducted in a private hospital in Belo Horizonte, MG, Brazil, which included all patients with infections caused by Enterobacteriaceae which carry the Klebsiella pneumoniae Carbapenemase gene. The data were collected by the Automated System of Hospital Infection Control and analyzed by descriptive statistics by the Epi Info 7 program. Results: Eighty-two (82) patients participated in the study. Klebsiella pneumoniae was the most frequent species (68%) isolated in blood (30%), bronchoalveolar lavage (22%) and urine (18%), while catheter-associated bloodstream infection (30%) predominated regarding topography. A case fatality rate of 62% is highlighted in evaluating the outcome. Conclusion: The resistance genes spread rapidly, limiting the antimicrobial options for treating infectious diseases. The epidemiological profile of Healthcare-Associated Infections found in this study can be prevented by prevention and infection control programs.

show abstract

Section: Discussionsupporting

confidence: 83%

Epidemiological profile of healthcare-associated infections caused by Carbapenemase-producing Enterobacteriaceae

Alvim

Couto

Gazzinelli

2019

Rev. esc. enferm. USP

View full text Add to dashboard Cite

show abstract

“…Determining resistance and susceptibility for imipenem-relebactam with disks and both strips for K. pneumoniae isolates was a challenge in some cases, because colonies grew within the zones of inhibition; the MIC was measured to the higher value or zone to the lower value. This has been described in other studies testing carbapenem resistance in K. pneumoniae carbapenemase (KPC)-producing Enterobacter isolates (21).…”

Section: Discussionsupporting

confidence: 54%

Imipenem-Relebactam Susceptibility Testing of Gram-Negative Bacilli by Agar Dilution, Disk Diffusion, and Gradient Strip Methods Compared with Broth Microdilution

et al. 2020

View full text Add to dashboard Cite

This study aimed to determine whether agar dilution, and research use only disk diffusion (Mast Group Ltd., Bootle Merseyside, UK), Etest (bioMérieux, Inc., Durham, NC), and MIC test strip (MTS) (Liofilchem, Inc., Waltham, MA) methods yield equivalent results to those of broth microdilution (BMD) for imipenem-relebactam susceptibility testing using a collection of 297 Gram-negative bacilli, including members of the order Enterobacterales and Pseudomonas aeruginosa, enriched for drug-resistance. MIC and disk diameter results were interpreted using United States Food and Drug Administration breakpoints. Overall, 76.8% of the isolates tested were susceptible to imipenem-relebactam by BMD. MIC values of agar dilution, Etest and MTS were not significantly different compared to BMD, although they tended to be 1 to 2 dilutions higher. Essential agreement was 95.6% for agar dilution, 90.6% for Etest, and 85.2% for MTS. Categorical agreement was 98.0% for agar dilution, 73.1% for disk diffusion, 96.3% for Etest, and 96.6% for MTS. In conclusion, agar dilution and Etest yielded comparable results to BMD for imipenem-relebactam.

show abstract

“…On par with the lower categorical agreement, the prevalence of mE's was higher than the other tests (11.7%). In contrast with some studies investigating different bacterial strains and antimicrobial agents (Biedenbach et al, 1993;Lehtopolku et al, 2012;Rechenchoski et al, 2017), the results of this study strengthen the validity of using the disk diffusion method for identifying resistance of APEC strains.…”

Section: Discussioncontrasting

confidence: 51%

“…Next to the paucity in gradient strip efficacy information, knowledge on the agreement between the other different AST methods is lacking for the specific APEC and ENRO combination. Most studies evaluating agreement between different AST methodologies have focused on human bacteria and fungi and antimicrobial agents frequently used in human medicine (Matar et al, 2003;Esteban et al, 2005;Rechenchoski et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Agreement of Quantitative and Qualitative Antimicrobial Susceptibility Testing Methodologies: The Case of Enrofloxacin and Avian Pathogenic Escherichia coli

et al. 2020

View full text Add to dashboard Cite

Avian pathogenic Escherichia coli (APEC) is the causal agent of colibacillosis, one of the most common bacterial infections in the poultry sector. Antimicrobial susceptibility testing (AST) is essential for rational and prudent antimicrobial therapy. Subsequently, uniformity in test results from the various testing methodologies used in diagnostic laboratories is pivotal. The aim of this study was therefore to evaluate the agreement between different AST methods in determining fluoroquinolone resistance in APEC. Twenty APEC isolates were selected and subjected to four different susceptibility tests: the quantitative microbroth dilution, agar dilution and gradient strip tests, and the qualitative disk diffusion method. The experiments were performed in triplicate. Categorical agreement, essential agreement and different errors were assessed. Moreover, agreement was also evaluated by calculating intraclass correlation coefficients (ICCs) for the quantitative tests and determining the Pearson correlation coefficients for the agreement between the disk diffusion method and the quantitative tests. Categorical agreement and essential agreement when compared with the microbroth technique ranged from 85-95% and 85-100%, respectively. No very major errors (false susceptible) and only one major error (false resistant) and minor errors (results involving an intermediary category) were detected. The calculated ICC values of the three quantitative tests fluctuated around 0.970 (range 0.940-0.988). There was a high negative correlation between the disk diffusion method and the other tests (correlation coefficients ranging from −0.979 to −0.940), indicating a clear inverse relationship between the minimum inhibitory concentration value and the zone diameter of growth inhibition. In conclusion, the overall agreement between the four different testing methodologies was very high. These results confirm the reliability of the disk diffusion and gradient strip test methods as substantiated alternatives, next to the gold standard agar and microbroth dilution, for fluoroquinolone susceptibility testing of APEC isolates.

show abstract

Antimicrobial activity evaluation and comparison of methods of susceptibility for Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacter spp. isolates

Cited by 27 publications

References 24 publications

Epidemiological profile of healthcare-associated infections caused by Carbapenemase-producing Enterobacteriaceae

Epidemiological profile of healthcare-associated infections caused by Carbapenemase-producing Enterobacteriaceae

Imipenem-Relebactam Susceptibility Testing of Gram-Negative Bacilli by Agar Dilution, Disk Diffusion, and Gradient Strip Methods Compared with Broth Microdilution

Agreement of Quantitative and Qualitative Antimicrobial Susceptibility Testing Methodologies: The Case of Enrofloxacin and Avian Pathogenic Escherichia coli

Contact Info

Product

Resources

About