Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis

Mirhosseini, Seyed Ali; Fooladi, Abbas Ali Imani; Amani, Jafar; Sedighian, Hamid

doi:10.1016/j.bjm.2016.04.033

Cited by 24 publications

(14 citation statements)

References 27 publications

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“…Such setups are reported to be 2–5 times more sensitive than direct and other ELISA types [ 119 ]. 10 3 CFU mL −1 of food-borne pathogenic Salmonella could be detected in indirect ELISA by targeting its membrane protein, bacterial flagellin FliC [ 120 ], while whole bacterial cell sandwich ELISA allowed detecting 10 3 CFU mL −1 of Brucella abortus and Brucella melitensis [ 121 ] and Bacillus cerus [ 122 ]. Further specificity and sensitivity of analysis can be controlled by a proper choice of Abs and Ab-replacing aptamers and of aptamer-reporter labels and nanomaterial-based labels [ 123 ].…”

Section: Immunoassays and Aptamer-based Assays With Optical Detectmentioning

confidence: 99%

Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

Jamal

Shipovskov

Ferapontova

2020

Sensors

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Microbiological safety of the human environment and health needs advanced monitoring tools both for the specific detection of bacteria in complex biological matrices, often in the presence of excessive amounts of other bacterial species, and for bacteria quantification at a single cell level. Here, we discuss the existing electrochemical approaches for bacterial analysis that are based on the biospecific recognition of whole bacterial cells. Perspectives of such assays applications as emergency-use biosensors for quick analysis of trace levels of bacteria by minimally trained personnel are argued.

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Section: Immunoassays and Aptamer-based Assays With Optical Detectmentioning

confidence: 99%

Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

Jamal

Shipovskov

Ferapontova

2020

Sensors

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“…human and animal hosts after the uptake of contaminated water and food such as poultry meat and eggs (Mirhosseini, Fooladi, Amani, & Sedighian, 2017;Tarabees, Elsayed, Shawish, Basiouni, & Shehata, 2017). The most commonly reported infectious disease caused by S. enteritidis is acute gastroenteritis (Muvhali, Smith, Rakgantso, & Keddy, 2017).…”

Section: S Enteritidis Induces Gastrointestinal and Systemic Diseasementioning

confidence: 99%

“…Salmonella enteritidis is a Gram‐negative bacterial pathogen that exists in the intestinal tract, skin, and feathers of living birds. S. enteritidis induces gastrointestinal and systemic diseases in human and animal hosts after the uptake of contaminated water and food such as poultry meat and eggs (Mirhosseini, Fooladi, Amani, & Sedighian, ; Tarabees, Elsayed, Shawish, Basiouni, & Shehata, ). The most commonly reported infectious disease caused by S. enteritidis is acute gastroenteritis (Muvhali, Smith, Rakgantso, & Keddy, ).…”

Section: Introductionmentioning

confidence: 99%

Increased growth rate and amikacin resistance of Salmonella enteritidis after one‐month spaceflight on China’s Shenzhou‐11 spacecraft

et al. 2019

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China launched the Tiangong‐2 space laboratory in 2016 and will eventually build a basic space station by the early 2020s. These spaceflight missions require astronauts to stay on the space station for more than 6 months, and they inevitably carry microbes into the space environment. It is known that the space environment affects microbial behavior, including growth rate, biofilm formation, virulence, drug resistance, and metabolism. However, the mechanisms of these alternations have not been fully elucidated. Therefore, it is beneficial to monitor microorganisms for preventing infections among astronauts in a space environment. Salmonella enteritidis is a Gram‐negative bacterial pathogen that commonly causes acute gastroenteritis in humans. In this study, to better understand the effects of the space environment on S. enteritidis, a S. enteritidis strain was taken into space by the Shenzhou‐11 spacecraft from 17 October 2016 to 18 November 2016, and a ground simulation with similar temperature conditions was simultaneously performed as a control. It was found that the flight strain displayed an increased growth rate, enhanced amikacin resistance, and some metabolism alterations compared with the ground strain. Enrichment analysis of proteome revealed that the increased growth rate might be associated with differentially expressed proteins involved in transmembrane transport and energy production and conversion assembly. A combined transcriptome and proteome analysis showed that the amikacin resistance was due to the downregulation of the oppA gene and oligopeptide transporter protein OppA. In conclusion, this study is the first systematic analysis of the phenotypic, genomic, transcriptomic, and proteomic variations in S. enteritidis during spaceflight and will provide beneficial insights for future studies on space microbiology.

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“…Enteritidis because of the shared O9 antigen in the three serotypes [8]. ELISA methods were subsequently developed to detect antibodies against LPS or FliC in avian sera [8–11], but problems of cross-reactivity could not be resolved because of antigen conservation in the different serotypes. Therefore, developing an ELISA assay specific to S .…”

Section: Introductionmentioning

confidence: 99%

Purification of recombinant IpaJ to develop an indirect ELISA-based method for detecting Salmonella enterica serovar Pullorum infections in chickens

Yue

Yin

et al. 2019

BMC Vet Res

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BackgroundSalmonella enterica serovar Pullorum is a host-restricted serotype causing infection in poultry. The pathogen can not only cause acute infection in young chicks with high mortality and morbidity, but also persist in adult chickens without evident clinical symptoms and lead to vertical transmission. To eradicate S. Pullorum in poultry farms, it is necessary to establish an efficient method to monitor the prevalence of the pathogen in adult chickens. The protein IpaJ is a specific immunogen in S. Pullorum and is not detected in closely related serotypes, such as S. Gallinarum and S. Enteritidis.ResultsIn the present study, IpaJ was expressed as a recombinant fusion protein MBP-IpaJ in E. coli. The purified MBP-IpaJ was used as a coating antigen to develop an indirect ELISA assay, which was applied to the detection of S. Pullorum infection in chickens. The indirect ELISA assay demonstrated that antibodies produced against IpaJ were detectable in antisera of chickens infected with S. Pullorum in the second week, stably increased until the tenth week, and persisted at a high level in the following two weeks. Furthermore, the ELISA method detected four positive samples out of 200 clinical antiserum samples collected from a poultry farm, and the positive samples were confirmed to be reacted with S. Pullorum using the standard plate agglutination test.ConclusionsThe established indirect ELISA using the IpaJ protein is a novel method for specific detection of S. Pullorum infection, and contribute to eradication of pullorum disease in the poultry industry.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1753-0) contains supplementary material, which is available to authorized users.

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Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis

Cited by 24 publications

References 27 publications

Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

Increased growth rate and amikacin resistance of Salmonella enteritidis after one‐month spaceflight on China’s Shenzhou‐11 spacecraft

Purification of recombinant IpaJ to develop an indirect ELISA-based method for detecting Salmonella enterica serovar Pullorum infections in chickens

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