Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar

Gonsalves, Camila Cristina; Borsoi, Anderlise; Perdoncini, Gustavo; Rodrigues, Laura Beatriz; Nascimento, Vladimir Pinheiro do

doi:10.1016/j.bjm.2016.04.025

Cited by 4 publications

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References 12 publications

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“…Wang and collaborators investigated the prevalence of Campylobacter from broiler chickens in slaughter houses in five Chinese provinces during 2008–2014, and isolated 977 C. jejuni (18.1%) and 1021 C. coli (19.0%) from 5,385 chickens ( Wang et al, 2016 ). In southern Brazil, samples from the broiler slaughtering process were analyzed to directly count Campylobacter and results showed that 72 and 38% were C. jejuni and C. coli , respectively ( Gonsalves et al, 2016 ). Similar results were demonstrated in the European Union, where 60.8% of broiler samples tested positive for C. jejuni , and 41.5% tested positive for C. coli ( European Food Safety Authority [EFSA], 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Characteristics of Campylobacter Throughout the Slaughter Process of Different Broiler Batches

et al. 2018

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Handling and consumption of chicken meat are risk factors for human campylobacteriosis. This study was performed to describe the Campylobacter population in broiler carcasses and environmental samples throughout the slaughter process. Moreover, the genetic diversity and antimicrobial resistance of the Campylobacter strains were evaluated. Cloacal swabs, samples from carcasses at different stages, and environmental samples were collected thrice from the different flocks at the same abattoir located in Central Jiangsu, China. Campylobacter isolated from the three batches (n = 348) were identified as Campylobacter jejuni (n = 117) and Campylobacter coli (n = 151) by multiplex PCR. Characterization by multilocus sequence typing revealed a specific genotype of Campylobacter for each batch. Antimicrobial sensitivity to 18 antibiotics were analyzed for all selected strains according to the agar diffusion method recommended by the Clinical and Laboratory Standards Institute. Antibiotic susceptibility tests indicated that the majority of the tested isolates were resistant to quinolones (>89.7%). Less resistance to macrolide (59.8%), gentamicin (42.7%), amikacin (36.8%) was observed. Results showed that 94.0% of the tested strains demonstrated multidrug resistance.

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Section: Discussionmentioning

confidence: 99%

Prevalence and Characteristics of Campylobacter Throughout the Slaughter Process of Different Broiler Batches

et al. 2018

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Prevalence and levels of Campylobacter in broiler chicken batches and carcasses in Ireland in 2017–2018

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Franklin-Hayes

Koolman

et al. 2022

International Journal of Food Microbiology

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Evaluation of different Campylobacter jejuni isolates to colonize the intestinal tract of commercial turkey poults and selective media for enumeration

et al. 2018

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Consumption of contaminated poultry products is the main source of human campylobacteriosis, for which Campylobacter jejuni is responsible for 90% of human cases. Although chickens are believed to be a main source of human exposure to C. jejuni, turkeys also contribute to cases of human infection. Little is known about the kinetics of C. jejuni intestinal colonization in turkeys, or best selective media for their recovery. Enumeration of C. jejuni from intestinal samples can be challenging because most selective Campylobacter media support the growth of non-Campylobacter organisms. In this study, we sought to compare a) C. jejuni isolates that persistently colonize different compartments of the poult intestinal tract, and b) selective media to enumerate C. jejuni from turkey intestinal samples. Three-week-old poults were orally colonized with C. jejuni isolates NCTC 11168 or NADC 20827 (isolated from a turkey flock). Mock-colonized poults were orally gavaged with uninoculated media. Poults were euthanized at d 3, 7, and 21 post colonization and direct plated on different selective Campylobacter media [Campy Line agar with sulfamethoxazole (CLA-S), CHROMagar Campylobacter (CAC) and Campy Cefex] for enumeration. Isolates NCTC 11168 and NADC 20827 poorly colonized the distal ileum. Both isolates colonized the colon, but the number of NADC 20827 significantly decreased at d 21. Isolates NCTC 11168 and NADC 20827 persistently colonized the cecum for up to 21 days. There was no significant difference in the Campylobacter amount recovered on CLA-S and CAC. Campy Cefex failed to prevent growth of background microbes to enumerate C. jejuni from turkey samples. Two independent PCR assays (multiplex PCR and qPCR) confirmed that colonies grown on CLA-S or CAC were C. jejuni. Data from this study demonstrated that isolates NCTC 11168 and NADC 20827 persistently colonized the cecum, and CLA-S or CAC were successful to enumerate Campylobacter from intestinal samples. These findings will be useful to evaluate the host response by C. jejuni in turkeys, and test pre-harvest strategies to reduce its colonization and promote food safety.

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Campylobacter in broiler slaughter samples assessed by direct count on mCCDA and Campy-Cefex agar

Cited by 4 publications

References 12 publications

Prevalence and Characteristics of Campylobacter Throughout the Slaughter Process of Different Broiler Batches

Prevalence and Characteristics of Campylobacter Throughout the Slaughter Process of Different Broiler Batches

Prevalence and levels of Campylobacter in broiler chicken batches and carcasses in Ireland in 2017–2018

Evaluation of different Campylobacter jejuni isolates to colonize the intestinal tract of commercial turkey poults and selective media for enumeration

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