Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement

Akbar, Shamsa; Sultan, Sikander

doi:10.1016/j.bjm.2016.04.009

Cited by 140 publications

(70 citation statements)

References 34 publications

(42 reference statements)

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“…resinovorans (12), Ps. fluorescens (13), Bacillus subtilis (13,14,15,16), Bacillus sp and Micrococcus sp (17,18), B. firmis (19), B. cereus (20), Stenotrophomonas (21), Serratia marcescens, Klebsiella oxytoca (22), Achromobacter sp, Ochrobactrum sp (23), Alcaligenes faecalis (24), Mesorhizobium (25), cellulomonas fimi (26), Gordonia (27), B. polymyxa (28), Kocuria sp (29), Staphylococcus sp, Streptococcus sp, azomonas sp, Flavobacterium sp (30) were extensively studied in degradation of chlorpyrifos.…”

Section: Issn: 2320-5407mentioning

confidence: 99%

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Isolation and Identification of Chlorpyrifos Degrading Bacteria From Agricultural Soil.

Hamsavathani¹,

Aysha²,

Ajith³

2017

IJAR

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The extensive use of pesticides in agricultural fields for pest control pose a serious problem in contaminating soil and water ecosystems. Chlorpyrifos is the major broad spectrum organophosphorus insecticide used in paddy fields against sucking, chewing, boring insects. Due to its toxicity and persistence in the environment, there is an immediate need to eliminate them from contaminated sites by biodegradation. Two pesticide degrading bacteria were screened and isolated from chlorpyrifos contaminated soil by enrichment culture technique and were identified as Kocuria kristinae and Staphylococcus aureus. The growth response and degradation of chlorpyrifos by the isolates in MSM broth supplemented with 0.5% chlorpyrifos was monitored every 48-72 hrs in spectrophotometer at 600nm. Kocuria sp showed maximum growth in 7 days than Staphylococcus aureus. The degradation efficiency of the strains were determined and estimated by the removal percentage of chlorpyrifos from the liquid culture. Both the isolate showed the degrading capability of chlorpyrifos in MSM. The isolate, S. aureus was more potent in degrading the 80% of the total compound from the media in 2 weeks of incubation than K. kristinae which shows 35 % of degradation. These results were further confirmed by GCMS, in which S. aureus has degraded 82.06% and K. kristinae has degraded 30.78% of chlorpyrifos in the medium. This study indicates that the isolate, Staphylococcus aureus is more potent in degrading chlorpyrifos in liquid culture and can also be used in bioremediation of chlorpyrifos contaminated soils.

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Section: Issn: 2320-5407mentioning

confidence: 99%

“…Growth response of the isolates and degradation were observed at every 48-72 hrs interval in spectrophotometer at 600 nm (23). The content of the flasks were checked by taking 5 ml of culture drawn from mineral based medium and centrifuged at 5000 rpm for 10 minutes.…”

Section: Degradation Of Chlorpyrifos:-mentioning

confidence: 99%

Isolation and Identification of Chlorpyrifos Degrading Bacteria From Agricultural Soil.

Hamsavathani¹,

Aysha²,

Ajith³

2017

IJAR

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“…strain C1, probably requiring only few days more to achieve complete CHL elimination. A study reported that A. xylosoxidans JCp4 was able to mineralize 100 mg L −1 CHL completely after ten days with only a transient accumulation of TCP [40]. Our work constitutes one of the few reports of Achromobacter as CHL-degraders.…”

Section: Discussionmentioning

confidence: 64%

Biopurification system as a source of pesticide-tolerant bacteria able to degrade the commonly used pesticides chlorpyrifos and iprodione

Díez

Lamilla

Leiva

et al. 2018

Preprint

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Intensive use of pesticides applied simultaneously in field to improve the effectiveness of pest control increase the environmental contamination, affecting the soil and water quality. Some of the commonly used pesticides are the insecticide chlorpyrifos and the fungicide iprodione; being thus critically essential to develop bioremediation methods to remove these contaminants by tolerant-bacteria. In this study we selected and characterized different pesticides-tolerant bacteria isolated from a biomixture of a biopurification system that had received continuous applications of a mixture of the pesticides chlorpyrifos and iprodione. Out of the 10 isolated bacterial colonies, only six strains presented adequate growth in presence of the both pesticides at 100 mg L-1. Biochemical and enzymatic characterization using API ZYM showed that all isolates (100%) were positive for esterase, leucine aminopeptidase, acid phosphatase, and naphthol-AS-BI-phosphohydrolase. According to the molecular level study of the 16S ribosomal gene and MALDI TOF/TOF MS, it was possible to determine that the isolated bacteria belong to the genera Pseudomonas, Rhodococcus and Achromobacter. Bacterial growth decreased proportionally (R2 > 0.96) as been as both pesticide concentrations increased from 10 to 100 mg L-1. Achromobacter sp. strain C1 showed the best chlorpyrifos removal (between 56–29%) after 120 h of incubation. On the other hand, the highest iprodione removal (between 91.2–98.9%) was observed for the Pseudomonas sp. strain C9, which was not detected after 48 h of incubation. According with their identification and ability to remove the contaminants, Achromobacter sp. strain C1 and Pseudomonas sp. strain C9 appear as promising microorganisms for their use in the treatment of matrices contaminated with chlorpyrifos, iprodione or their mixture. The results of this study will help to improve current technologies for the biodegradation of this commonly used insecticide and fungicide, in order to give a response to the problem of contamination by pesticides.

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“…Biodegradation of chlorpyrifos and the bacteria involved in this process are well studied [2,14,31]. However, biodegradation of chlorpyrifos in relation to the metabolic nature of bacterial community is not explored.…”

Section: Discussionmentioning

confidence: 99%

“…Chlorpyrifos and other insecticides (carbofuran, thiomethoxam, imidacloprid) inhibit phosphate solubilising bacteria [28]. Chlorpyrifos also inhibits beneficial plant growth promoting microorganisms in soil [2]. In natural ecosystem chlorpyrifos is degraded to diethylthiophosphoric acid (DETP) and 3,5,6-trichloro-2-pyridinol (TCP) [25].…”

Section: Introductionmentioning

confidence: 99%

Chlorpyrifos biodegradation in relation to metabolic attributes and 16S rRNA gene phylogeny of bacteria in a tropical vertisol

et al. 2019

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It is predicted that use of insecticide chlorpyrifos will increase in near future due to intensive agricultural practice and climate change. The present study envisages establishing linkages among biodegradation potential, carbon metabolism and enzymatic attributes of bacteria involved in chlorpyrifos biodegradation. Soil samples from an experimental field were incubated with chlorpyrifos (10 µg g −1 soil). Chlorpyrifos biodegradation rate was 0.34 ± 0.04 ug g −1 soil d −1 . Culturable bacteria were isolated and identified by 16S rRNA gene sequencing. DNA sequences were 96-100% similar to the GenBank bacterial DNA sequences. The bacterial community was predominated by Arthrobacter phenanthrenivorans (23%). Followed by Arthrobacter globiformis and Bacillus flexus represented 15% each. Microbacterium paraoxydans, Bacillus megaterium, Bacillus soli strain R, Bacillus drentensis, and Bacillus koreensis represented 8% of total bacteria. The carbon source utilization pattern and enzymatic activities of the bacterial isolates were estimated. The isolates preferred to metabolize trehalose and mannose over other C sources. These bacteria exhibited decarboxylase, oxidase, catalase, cellulose, deaminase and nitrate reductase activities. A PCA analysis based on various metabolic attributes explained 32.49% variance by PC1 and 16.46% variance by PC2. PCA highlights that the bacterial isolates placed distantly from those of simple sugar utilizers, probably use chlorpyrifos as C substrate. Correspondence analysis outlaid decarboxylase, catalase, and oxidase as the key enzymatic activities of the bacterial isolates for chlorpyrifos biodegradation.

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Soil bacteria showing a potential of chlorpyrifos degradation and plant growth enhancement

Cited by 140 publications

References 34 publications

Isolation and Identification of Chlorpyrifos Degrading Bacteria From Agricultural Soil.

Isolation and Identification of Chlorpyrifos Degrading Bacteria From Agricultural Soil.

Biopurification system as a source of pesticide-tolerant bacteria able to degrade the commonly used pesticides chlorpyrifos and iprodione

Chlorpyrifos biodegradation in relation to metabolic attributes and 16S rRNA gene phylogeny of bacteria in a tropical vertisol

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