Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi

Colla, Luciane Maria; Primaz, Andreiza Lazzarotto; Benedetti, Silvia; Loss, Raquel Aparecida; Lima, Marieli de; Reinehr, Christian Oliveira; Bertolin, Telma Elita; Costa, Jorge Alberto Vieira

doi:10.1016/j.bjm.2016.01.028

Cited by 87 publications

(52 citation statements)

References 29 publications

(37 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, microbial lipases form the most widely used class of enzymes due to their selectivity, lower production cost, ease of genetic manipulation, high productivity, regiospecificity, stereospecificity, broad substrate specificity and ability to catalyze heterogeneous reactions at the interface of water soluble and water insoluble systems [8,[11][12][13]. Microorganisms such as bacteria, yeast, fungi and actinomycetes have been reported as potential producers of lipase [14][15][16][17]. They are found in different habitats, including industrial wastes, vegetable oil processing industries, dairy industries and oil-contaminated sites, among others [18].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, RSM has been successfully applied to evaluate and optimize the effect of process parameters in the production of lipase [28,31,32]. A central composite design (CCD) is the most commonly used response surface design when the experimental design is defined by the upper and lower limits of each parameter [16,33]. Several bioprocess parameters have been optimized for enhanced lipase production using CCD [15,16,33,34].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of culture conditions for enhanced lipase production by an indigenousBacillus aryabhattaiSE3-PB using response surface methodology

Adetunji

Olaniran

2018

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Lipases are enzymes that hydrolyze fats into fatty acids and glycerol at the water-lipid interface and are also involved in a variety of bioconversion reactions in non-aqueous and micro-aqueous environments. In this study, we optimized the culture conditions for extracellular lipase production by an indigenous lipase-producing bacterial strain isolated from lipid-rich wastewater, using response surface methodology. The studied isolate was identified as Bacillus aryabhattai SE3-PB by polymerase chain reaction and analysis of 16S rDNA. Sunflower oil was found to induce maximum lipase production. Face centered central composite design revealed that temperature (40 C), pH (7.6), inoculum volume (2.8%, v/v), agitation (193 rpm) and inducer oil concentration (2%, v/v) significantly influenced lipase production at the respective optimum conditions. The coincidence of observed lipase production (264.02 ± 1.94 U/mL) with predicted lipase yield (265.82 U/mL) coupled with a high correlation coefficient (R 2 ¼ 0.9919, P < 0.01) confirmed the validity of the model. A 7.2-fold increase in lipase production was obtained in the optimized medium compared to the basal medium. These findings provide the first report on lipase production and optimization by B. aryabhattai SE3-PB and suggest a rational choice of optimum processing conditions for commercial lipase production by B. aryabhattai SE3-PB.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of culture conditions for enhanced lipase production by an indigenousBacillus aryabhattaiSE3-PB using response surface methodology

Adetunji

Olaniran

2018

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

show abstract

“…Interactions among the three factors are illustrated (Figure 6). Many authors applied Plackett–Burman experimental design for the enhancement of enzyme production (Collaa et al, 2016; Kai and Peisheng, 2016; Vasiee et al, 2016; Ebrahimipour et al, 2017; Mazhar et al, 2017). …”

Section: Resultsmentioning

confidence: 99%

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

2018

View full text Add to dashboard Cite

Lipases are enzymes that have the potential to hydrolyze triacylglycerol to free fatty acids and glycerol and have various applications. The aim of the present study was to isolate and screen marine bacteria for lipase production, optimize the production, and treat wastewater. A total of 20 marine bacterial isolates were obtained from the Mediterranean Sea and were screened for lipase production. All isolates were found to have lipolytic ability. The differences between the isolates were studied using RAPD-PCR. The most promising lipase producer (isolate 3) that exhibited the highest lipolytic hydrolysis (20 mm) was identified as Bacillus cereus HSS using 16S rDNA analysis and had the accession number MF581790. Optimization of lipase production was carried out using the Plackett–Burman experimental design with cotton seed oil as the inducer under shaking conditions at 10°C. The most significant factors that affected lipase production were FeSO4, KCl, and oil concentrations. By using the optimized culture conditions, the lipase activity increased by 1.8-fold compared with basal conditions. Immobilization by adsorption of cells on sponge and recycling raised lipase activity by 2.8-fold compared with free cells. The repeated reuse of the immobilized B. cereus HSS maintained reasonable lipase activity. A trial for the economic treatment of oily wastewater was carried out. Removal efficiencies of biological oxygen demand, total suspended solids, and oil and grease were 87.63, 90, and 94.7%, respectively, which is promising for future applications.

show abstract

“…; Colla et al . ). The need for micro‐organisms to utilize water‐immiscible substrates prompts them to synthesize biosurfactants along with lipases.…”

Section: Promising Strategiesmentioning

confidence: 97%

Biosurfactant production: emerging trends and promising strategies

2018

View full text Add to dashboard Cite

Biosurfactants are economically most sought after biotechnological compounds of the 21st century. However, inefficient bioprocessing has mitigated the economical commercial production of these compounds. Although much work is being done on the use of low-cost substrates for their production, a paucity of literature exists on the upcoming bioprocess optimization strategies and their successes and potential for economical biosurfactant production. This review discusses some of the latest developments and most promising strategies to enhance and economize the biosurfactant production process. Recent market analysis, developments in the field of optimally formulated cost credit substrates for enhanced product formation and subsequent process economization are few of the critical aspects highlighted here. Use of nanoparticles and coproduction of biosurfactant along with other commercially important compounds like enzymes, are other upcoming bioprocess intensification strategies. The recent developments discussed here would not only give an overview of pertinent parameters for economic biosurfactant production but would also bring to fore multiple strategies that would open up new avenues of research on biosurfactant production. This would go a long way in making biosurfactants a commercially successful compound of the current century.

show abstract

Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi

Cited by 87 publications

References 29 publications

Optimization of culture conditions for enhanced lipase production by an indigenousBacillus aryabhattaiSE3-PB using response surface methodology

Optimization of culture conditions for enhanced lipase production by an indigenousBacillus aryabhattaiSE3-PB using response surface methodology

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

Biosurfactant production: emerging trends and promising strategies

Contact Info

Product

Resources

About