Alternariol 9-methyl ether from the endophytic fungus Alternaria sp. Samif01 and its bioactivities

Lou, Jingfeng; Rong, Yu; Wang, Xiaohan; Mao, Ziling; Fu, Linyun; Liu, Yang; Zhou, Ligang

doi:10.1016/j.bjm.2015.11.004

Cited by 45 publications

(20 citation statements)

References 26 publications

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“…2). Although the nematicidal activity of secondary compounds from Alternaria species has been explored (Lou et al 2016), our study is the first to illustrate the potential ecological significance of specific Alternaria strains on nematodes in planta using live plant assays, with both positive and negative effects on root-knot nematode galling. Similarly, some Cladosporium strains have been shown to produce secondary metabolites with nematicidal or insecticidal properties (Qureshi et al 2012;Singh et al 2016), but our results provide the first examples of negative in planta effects of multiple strains on nematodes.…”

Section: Discussionmentioning

confidence: 99%

Tapping Into the Cotton Fungal Phytobiome for Novel Nematode Biological Control Tools

Zhou

Verma

Wheeler

et al. 2020

Phytobiomes Journal

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A number of fungi have been shown to have negative effects on plant-parasitic nematodes. Most of these fungi have been isolated from soil, plant roots, or nematodes themselves. Fungi associated with crops can provide a diverse pool of candidates to test for antagonistic effects against plant parasites and other stressors. We used a hierarchical two-tiered approach to evaluate the efficacy and repeatability of 55 strains of fungi originally isolated as foliar facultative endophytes from upland cotton (Gossypium hirsutum) along with one commercial isolate of Beauveria bassiana for in planta antagonistic effects on root-knot nematodes (Meloidogyne incognita). All fungi were inoculated to cotton using a seed treatment. The number of root galls was quantified 3 weeks after egg inoculation of cotton seedlings. The majority of the fungi tested reduced the number of root galls relative to those on untreated control plants. To assess repeatability, 22 strains that exhibited the strongest reductions in gall numbers were further tested in replicate follow-up assays. Ninety-five percent (21/22) of these retested strains significantly reduced galling in the follow-up assay. Strains that reduced galling the most belonged to the genera Alternaria, Chaetomium, Cladosporium, Diaporthe, Epicoccum, Gibellulopsis, and Purpureocillium. On the contrary, three strains in the genera Alternaria and Curvularia significantly increased gall numbers. Our results indicate that a large proportion of the fungal strains originally isolated from cotton as naturally occurring foliar facultative endophytes are capable of reducing root-knot nematode infection when applied back to the plant as a seed treatment. These findings help establish a rich pool of candidate fungi for further evaluation as novel biological control tools against root-knot nematodes in cotton and other plants.

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Section: Discussionmentioning

confidence: 99%

Tapping Into the Cotton Fungal Phytobiome for Novel Nematode Biological Control Tools

Zhou

Verma

Wheeler

et al. 2020

Phytobiomes Journal

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“…The pure compound (AME) from the strain MGTMMP031 exhibited potential activity against B. cereus and K. pneumoniae and inhibited the growth of S. typhi , E. coli and S. aureus . Studies have shown that AME from endophytic fungus has potential bioactivity against micro‐organisms (Lou et al ). The effect of purified compound AME on cell cycle progression was determined in exponentially dividing cultures of cell lines.…”

Section: Discussionmentioning

confidence: 99%

Purification, crystallization and anticancer activity evaluation of the compound alternariol methyl ether from endophytic fungiAlternaria alternata

et al. 2019

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Aims: Medicinal plant-associated endophytic fungi are important sources of precious bioactive compounds, contributing more than 80% of the natural drugs for various ailments. The present study was aimed at evaluating the anticancer activity of the crystallized compound alternariol methyl ether (AME) against hepatocellular carcinoma (HCC) both in vitro and in vivo from an endophytic fungus residing in the medicinal plant Vitex negundo. Methods and Results: The secondary metabolites from the endophytic fungus Alternaria alternata MGTMMP031 were isolated. Purification and characterization of the compound was performed and the potential compound was identified as AME. The crystal structure of AME was unambiguously confirmed by X-ray analysis. AME has been checked for its antibacterial and anticancer properties which showed its effectiveness against various bacteria and demonstrated marked anti-proliferative activity against the human HCC cells (HUH-7) both in vitro and in vivo. Mode of actions included cell cycle arrest, reducing the level of markers enzymes of liver cancer and preventing tumour growth. Conclusions: Alternariol methyl ether acts as a potential therapeutic target against HCC. The compound was isolated and the crystal structure was obtained for the first time from the endophytic fungus A. alternata MGTMMP031. In the present study, the crystallized structure of AME was obtained by slow evaporation technique. It can be concluded that AME acts as a potential therapeutic target against HCC. Significance and Impact of the Study: Endophytic fungi residing in the medicinal plants have strong biological significance and bioactive compounds from these fungi provide better therapeutic targets against diseases.

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“…Thin layer chromatography (TLC) was carried out with hexane: ethyl acetate (2:3) solvent system in a pre-coated silica gel TLC plates of grade F274 (E-Merck, Germany) [11]. The eluted plates were dried completely and the chromatograms were visualized under UV at 365 nm and the movement of the metabolites along with solvent was measured (R f value).…”

Section: Characterization Of Alternariol Like Compoundmentioning

confidence: 99%

Bioactive potential of secondary metabolites derived from medicinal plant endophytes

Palanichamy

Krishnamoorthy

Kannan

et al. 2018

Egyptian Journal of Basic and Applied Sciences

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Medicinal plants have been used as an alternative medicine to promote human health and longevity in many regions of the world since ancient times. In recent years, many novel secondary metabolites from higher fungi have been isolated and reported to provide lead compounds for new drug discovery like antimicrobial, antioxidant, and anticancer agents, etc. A total of 42 endophytic fungi were isolated from 11 different medicinal plants collected from Tamil Nadu, India. The capability of medicinal plant endophytic fungi for the production of bioactive secondary metabolites was evaluated under in vitro conditions. The most frequently isolated fungi were Alternaria sp. Fusarium sp. and mycelia sterilia. The bioactive secondary metabolites of these endophytes were obtained by liquid-liquid extraction with ethyl acetate. Among which 15 showed antimicrobial activity towards potential human pathogens such as Escherichia coli, Salmonella typhi, Proteus mirabilis, Staphylococcus aureus, and Corynebacterium diphtheriae with variable zone of inhibition. Maximum secondary metabolite production with good mycelial growth and antimicrobial activity was observed in Alternaria sp. MGTMMP031. Moreover, in-vitro antioxidant and anticancer activity were also evaluated. The crude secondary metabolite was characterized by ultraviolet spectrum (UV), infrared spectrum (FT-IR) and thin layer chromatography (TLC) with R f value of 0.35 and the bioactive secondary metabolite was identified as alternariol methyl ether. The results concluded that secondary metabolite from Alternaria sp. may represent a potential ingredient for pharmaceutical application and are worthy of future study.

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Alternariol 9-methyl ether from the endophytic fungus Alternaria sp. Samif01 and its bioactivities

Cited by 45 publications

References 26 publications

Tapping Into the Cotton Fungal Phytobiome for Novel Nematode Biological Control Tools

Tapping Into the Cotton Fungal Phytobiome for Novel Nematode Biological Control Tools

Purification, crystallization and anticancer activity evaluation of the compound alternariol methyl ether from endophytic fungiAlternaria alternata

Bioactive potential of secondary metabolites derived from medicinal plant endophytes

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