Genotypes of Epstein–Barr virus (EBV1/EBV2) in individuals with infectious mononucleosis in the metropolitan area of Belém, Brazil, between 2005 and 2016

Monteiro, Talita Antônia Furtado; Costa, Iran Barros; Costa, Igor Brasil; Corrêa, Thaís Letícia dos Santos; Coelho, Beatriz Monteiro Rodrigues; Silva, Amanda Emanuelle Santos; Ramos, Francisco Lúzio de Paula; Filho, Arnaldo Jorge Martins; Monteiro, Jacqueline Cortinhas; Siqueira, Jones Anderson Monteiro; Gabbay, Yvone Benchimol; Sousa, Rita Catarina Medeiros

doi:10.1016/j.bjid.2020.06.004

Cited by 16 publications

(10 citation statements)

References 23 publications

(25 reference statements)

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“…The studies included a total number of 3,924 patients. Five studies evaluated only adult population [4][5][6][7][8] ; ten studies were on paediatric population (defined as under 18 years) [9][10][11][12][13][14][15][16][17][18] ; ten studies included both adult and children [19][20][21][22][23][24][25][26][27][28] ; eight studies did not report on the participants' age [29][30][31][32][33][34][35][36] .…”

Section: Resultsmentioning

confidence: 99%

The utility of liver function tests and abdominal ultrasound in infectious mononucleosis -- A systematic review

Tan¹,

Wilkinson

Edafe

2022

Preprint

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Introduction: A large proportion of patients with infectious mononucleosis (IM) have abnormal liver function tests (LFT) at presentation. There is no guideline regarding the management and follow-up of these patients. Some patients also have abdominal ultrasound due to deranged LFT, the need for this practice is unclear. The aim of this systematic review was to evaluate the evidence base on LFT assessment in IM, time to resolution of derangement, and the role of abdominal ultrasound. Methods: A systematic search of PubMed, EMBASE and the Cochrane library was done. Two authors independently screened records for eligibility using pre-defined criteria. We included both adult and paediatric populations. Quality assessment of included studies was done. Results: A total of 3924 patients were included from 32 studies. A combination of typical clinical features, heterophile antibodies and EBV-specific antibodies were used to ascertain diagnosis. The following proportion of patients had abnormal LFTs: AST (57%); ALT (62%); ALP (65%); Bilirubin (16%); GGT (41%). Reported median (i.q.r.) time to resolution of LFT was 8 (6–12) weeks. Maximum time to resolution was >6 months. Clinical hepatomegaly and splenomegaly were found in 35% and 44% of patients respectively. Enlarged liver and spleen on ultrasound were seen in 16/29 (55%) and 38/38 (100%) of patients respectively. There were no reports of decompensated liver disease. Conclusion: Derangement in LFTs can persist over six months from initial presentation in IM. However, this is self-limiting. The evidence suggests serial liver function assessments and ultrasound abdomen are not required in immunocompetent patients with subclinical derangement in LFTs.

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Section: Resultsmentioning

confidence: 99%

The utility of liver function tests and abdominal ultrasound in infectious mononucleosis -- A systematic review

Tan¹,

Wilkinson

Edafe

2022

Preprint

View full text Add to dashboard Cite

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“…For all PCR analysis, water was used as negative control, and B958 and P3HR1 viral DNA were used as positive controls. The B958 and P3HR1 viral strains were kindly provided by Dr. Talita A. F. Monteiro (Federal University of Pará, PA, Brazil) and were described elsewhere [47]. Samples were defined as negative if the C T values exceeded 40 cycles.…”

Section: Methodsmentioning

confidence: 99%

Impact of Epstein-Barr virus co-infection on natural acquired Plasmodium vivax antibody response

Dias

Guimarães

Barcelos

et al. 2022

Preprint

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Background: The simultaneous infection of Plasmodium falciparum and Epstein-Barr virus (EBV) could promote the development of the aggressive endemic Burkitt's Lymphoma (eBL) in children living in P. falciparum holoendemic areas. While it is well-established that eBL is not related to other human malaria parasites, the impact of EBV infection on the generation of human malaria immunity remains largely unexplored. Considering that this highly prevalent herpesvirus establishes a lifelong persistent infection on B-cells with possible influence on malaria immunity, we hypothesized that EBV co-infection could have impact on the naturally acquired antibody responses to P. vivax, the most widespread human malaria parasite. Methodology/Principal Findings: The study design involved three cross-sectional surveys at six-month intervals (baseline, 6 and 12 months) among long-term P. vivax exposed adults living in the Amazon rainforest. The approach focused on a group of malaria-exposed individuals whose EBV-DNA (amplification of balf-5 gene) was persistently detected in the peripheral blood (PersV DNA, n=27), and an age-matched malaria-exposed group whose EBV-DNA could never be detected during the follow-up (NegV DNA, n=29). During the follow-up period, the serological detection of EBV antibodies to lytic/latent viral antigens showed that IgG antibodies to viral capsid antigen (VCA-p18) were significantly different between groups (PersV DNA > NegV DNA ). A panel of blood-stage P. vivax antigens covering a wide range of immunogenicity confirmed that in general PersV DNA group showed low levels of antibodies as compared with NegV DNA. Interestingly, more significant differences were observed to a novel DBPII immunogen, named DEKnull-2, which has been associated with long-term neutralizing antibody response. Differences between groups were less pronounced with blood-stage antigens (such as MSP1-19) whose levels can fluctuate according to malaria transmission. Conclusions/Significance: In a proof-of-concept study we provide evidence that a persistent detection of EBV DNA in peripheral blood of adults in a P. vivax semi-immune population may impact the long-term immune response to major malaria vaccine candidates.

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“…For all PCR analyses, water was used as negative control, and B958 and P3HR1 viral DNA were used as positive controls. The B958 and P3HR1 viral strains were kindly provided by Dr. Talita A. F. Monteiro (Federal University of Pará, PA, Brazil) and were described elsewhere [ 53 ]. Samples were defined as negative if the CT values exceeded 40 cycles.…”

Section: Methodsmentioning

confidence: 99%

Impact of Epstein-Barr virus co-infection on natural acquired Plasmodium vivax antibody response

et al. 2022

View full text Add to dashboard Cite

Background The simultaneous infection of Plasmodium falciparum and Epstein-Barr virus (EBV) could promote the development of the aggressive endemic Burkitt’s Lymphoma (eBL) in children living in P. falciparum holoendemic areas. While it is well-established that eBL is not related to other human malaria parasites, the impact of EBV infection on the generation of human malaria immunity remains largely unexplored. Considering that this highly prevalent herpesvirus establishes a lifelong persistent infection on B-cells with possible influence on malaria immunity, we hypothesized that EBV co-infection could have impact on the naturally acquired antibody responses to P. vivax, the most widespread human malaria parasite. Methodology/Principal findings The study design involved three cross-sectional surveys at six-month intervals (baseline, 6 and 12 months) among long-term P. vivax exposed individuals living in the Amazon rainforest. The approach focused on a group of malaria-exposed individuals whose EBV-DNA (amplification of balf-5 gene) was persistently detected in the peripheral blood (PersVDNA, n = 27), and an age-matched malaria-exposed group whose EBV-DNA could never be detected during the follow-up (NegVDNA, n = 29). During the follow-up period, the serological detection of EBV antibodies to lytic/ latent viral antigens showed that IgG antibodies to viral capsid antigen (VCA-p18) were significantly different between groups (PersVDNA > NegVDNA). A panel of blood-stage P. vivax antigens covering a wide range of immunogenicity confirmed that in general PersVDNA group showed low levels of antibodies as compared with NegVDNA. Interestingly, more significant differences were observed to a novel DBPII immunogen, named DEKnull-2, which has been associated with long-term neutralizing antibody response. Differences between groups were less pronounced with blood-stage antigens (such as MSP1-19) whose levels can fluctuate according to malaria transmission. Conclusions/Significance In a proof-of-concept study we provide evidence that a persistent detection of EBV-DNA in peripheral blood of adults in a P. vivax semi-immune population may impact the long-term immune response to major malaria vaccine candidates.

show abstract

Genotypes of Epstein–Barr virus (EBV1/EBV2) in individuals with infectious mononucleosis in the metropolitan area of Belém, Brazil, between 2005 and 2016

Cited by 16 publications

References 23 publications

The utility of liver function tests and abdominal ultrasound in infectious mononucleosis -- A systematic review

The utility of liver function tests and abdominal ultrasound in infectious mononucleosis -- A systematic review

Impact of Epstein-Barr virus co-infection on natural acquired Plasmodium vivax antibody response

Impact of Epstein-Barr virus co-infection on natural acquired Plasmodium vivax antibody response

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