Yellow fever outbreak in Brazil, 2017

Goldani, Luciano Zubaran

doi:10.1016/j.bjid.2017.02.004

Cited by 59 publications

(38 citation statements)

References 8 publications

(8 reference statements)

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“…In addition to ZIKV and DENV, the ongoing threat of a Yellow Fever outbreak in Brazil (Goldani, 2017) and the re-emergence of Ebola in West Africa (Dudas et al, 2017) could lead to substantial worldwide morbidity and mortality. Because our epitope selection method does not rely on any characteristics intrinsic to ZIKV or flaviviruses but instead leverages well-defined structural metrics, we believe that it can be applied to any virus assembly, which can then guide appropriate antibody engineering efforts regardless of the platforms that might be used to actually generate the antibody.…”

Section: Discussionmentioning

confidence: 99%

Rational Engineering and Characterization of an mAb that Neutralizes Zika Virus by Targeting a Mutationally Constrained Quaternary Epitope

Tharakaraman

Watanabe

Chan

et al. 2018

Cell Host & Microbe

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Following the recent emergence of Zika virus (ZIKV), many murine and human neutralizing anti-ZIKV antibodies have been reported. Given the risk of virus escape mutants, engineering antibodies that target mutationally constrained epitopes with therapeutically relevant potencies can be valuable for combating future outbreaks. Here, we applied computational methods to engineer an antibody, ZAb_FLEP, that targets a highly networked and therefore mutationally constrained surface formed by the envelope protein dimer. ZAb_FLEP neutralized a breadth of ZIKV strains and protected mice in distinct in vivo models, including resolving vertical transmission and fetal mortality in infected pregnant mice. Serial passaging of ZIKV in the presence of ZAb_FLEP failed to generate viral escape mutants, suggesting that its epitope is indeed mutationally constrained. A single-particle cryo-EM reconstruction of the Fab-ZIKV complex validated the structural model and revealed insights into ZAb_FLEP’s neutralization mechanism. ZAb_FLEP has potential as a therapeutic in future outbreaks.

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Section: Discussionmentioning

confidence: 99%

Rational Engineering and Characterization of an mAb that Neutralizes Zika Virus by Targeting a Mutationally Constrained Quaternary Epitope

Tharakaraman

Watanabe

Chan

et al. 2018

Cell Host & Microbe

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“…Broad‐spectrum robust PRT represents an attractive alternative to blood screening using individual or even multiplex NAT assays in areas where arboviruses are often cocirculating and is a proactive strategy that seems more sustainable as pathogens emerge or reemerge . In addition for DENV, due to its mode of action, the PRT system using amotosalen/UVA was shown efficient at inactivating DENV, regardless of the serotype .…”

Section: Discussionmentioning

confidence: 99%

Pathogen inactivation of Dengue virus in red blood cells using amustaline and glutathione

et al. 2017

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BACKGROUND Dengue virus (DENV) is an arbovirus primarily transmitted through mosquito bite; however, DENV transfusion‐transmitted infections (TTIs) have been reported and asymptomatic DENV RNA–positive blood donors have been identified in endemic countries. DENV is considered a high‐risk pathogen for blood safety. One of the mitigation strategies to prevent arbovirus TTIs is pathogen inactivation. In this study we demonstrate that the amustaline and glutathione (S‐303/GSH) treatment previously found effective against Zika virus in red blood cells (RBCs) is also effective in inactivating DENV. STUDY DESIGN AND METHODS Red blood cells were spiked with high levels of DENV. Viral RNA loads and infectious titers were measured in the untreated control and before and after pathogen inactivation treatment of RBC samples. DENV infectivity was also assessed over five successive cell culture passages to detect any potential residual replicative virus. RESULTS The mean ± SD DENV titer in RBCs before inactivation was 6.61 ± 0.19 log 50% tissue culture infectious dose (TCID50)/mL and the mean viral RNA load was 8.42 log genome equivalents/mL. No replicative DENV was detected either immediately after completion of treatment using S‐303/GSH or after cell culture passages. CONCLUSION Treatment using S‐303/GSH inactivated high levels of DENV in RBCs to the limit of detection. In combination with previous studies showing the effective inactivation of DENV in plasma and platelets using the licensed amotosalen/UVA system, this study demonstrates that high levels of DENV can be inactivated in all blood components.

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“…Most yellow fever entries occurred in 2016 and early 2017, corresponding to the outbreaks in Angola and the Democratic Republic of the Congo, and Brazil, respectively ( Figure 6) (Kraemer et al, 2017;Goldani, 2017). EI activities first picked up a ProMED report about yellow fever in Angola on January 22, 2016, followed by a report from a NOR the same day.…”

Section: Yellow Fevermentioning

confidence: 99%

Identifying potential emerging threats through epidemic intelligence activities—looking for the needle in the haystack?

Wilburn¹,

O’Connor²,

Walsh³

et al. 2019

International Journal of Infectious Diseases

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A B S T R A C TBackground: Epidemic intelligence (EI) for emerging infections is the process of identifying key information on emerging infectious diseases and specific incidents. Automated web-based infectious disease surveillance technologies are available; however, human input is still needed to review, validate, and interpret these sources. In this study, entries captured by Public Health England's (PHE) manual event-based EI system were examined to inform future intelligence gathering activities. Methods: A descriptive analysis of unique events captured in a database between 2013 and 2017 was conducted. The top five diseases in terms of the number of entries were described in depth to determine the effectiveness of PHE's EI surveillance system compared to other sources. Results: Between 2013 and 2017, a total of 22 847 unique entries were added to the database. The top three initial and definitive information sources varied considerably by disease. Ebola entries dominated the database, making up 23.7% of the total, followed by Zika (11.8%), Middle East respiratory syndrome (6.7%), cholera (5.5%), and yellow fever and undiagnosed morbidity (both 3.3%). Initial reports of major outbreaks due to the top five disease agents were picked up through the manual system prior to being publicly reported by official sources. Conclusions: PHE's manual EI process quickly and accurately detected global public health threats at the earliest stages and allowed for monitoring of events as they evolved.Crown

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Yellow fever outbreak in Brazil, 2017

Cited by 59 publications

References 8 publications

Rational Engineering and Characterization of an mAb that Neutralizes Zika Virus by Targeting a Mutationally Constrained Quaternary Epitope

Rational Engineering and Characterization of an mAb that Neutralizes Zika Virus by Targeting a Mutationally Constrained Quaternary Epitope

Pathogen inactivation of Dengue virus in red blood cells using amustaline and glutathione

Identifying potential emerging threats through epidemic intelligence activities—looking for the needle in the haystack?

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