Modulation of drug resistance and biofilm formation of Staphylococcus aureus isolated from the oral cavity of Tunisian children

Zmantar, Tarek; Slama, Rihab Ben; Fdhila, Kais; Kouidhi, Bochra; Bakhrouf, Amina; Chaieb, Kamel

doi:10.1016/j.bjid.2016.10.009

Cited by 12 publications

(3 citation statements)

References 30 publications

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“…aureus strain with the developed assay. The observed antimicrobial activity (Table , entries 14–17) is comparable to that described in the literature, i.e., MIC = 0.5–2 μg/mL for vancomycin ( 7 ), − 2–16 μg/mL for benzalkonium chloride ( 8 ), − 3.91–16 μg/mL for chloramphenicol ( 9 ), , and 25–32 μg/mL for sulfamethoxazole ( 10 ). , In our assay, the S. aureus strain is thus not susceptible but intermediate and even resistant to these commercial agents, while it is susceptible to Cu 2 L P 60% (Figure ) and all other microgels (Table ).…”

Section: Resultssupporting

confidence: 85%

Antimicrobial Activity of Microgels with an Immobilized Copper(II) Complex Linked to Cross-Linking and Composition

Sharma

Clem

Perez

et al. 2020

ACS Appl. Bio Mater.

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The resistance of many bacteria against currently available antimicrobial agents is increasing worldwide at an alarming pace. The described structure–activity relationship study was prompted by the extraordinary ability of water-dispersed microgels to hydrolyze glycosidic bonds similar to building blocks of the peptidoglycan layer of Gram-positive bacteria. The results establish polyacrylate microgels with embedded copper(II) complex as antimicrobial agents. The systematic study reveals that Staphylococcus aureus is susceptible to the microgels, while common commercial agents are found intermediate or resistant. In particular, a microgel with 60 mol % of cross-linking, Cu 2 LP60%, shows intriguing bactericidal activity at 1 μg/mL, while vancomycin requires a 4-fold higher dose, i.e., 4 μg/mL, for the same effect. The minimum inhibitory concentration of Cu 2 LP60% was determined as low as 0.64 μg/mL. Excellent stability of the poly(acrylate) microgels was observed by negative zeta potentials in nanopure water and aqueous sodium dodecyl sulfate solution. The composition of the microgel matrix with embedded binuclear metal complex was shown to be responsible for the antimicrobial activity, while the aqueous buffer–surfactant solution is not.

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Section: Resultssupporting

confidence: 85%

Antimicrobial Activity of Microgels with an Immobilized Copper(II) Complex Linked to Cross-Linking and Composition

Sharma

Clem

Perez

et al. 2020

ACS Appl. Bio Mater.

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“…under aerobic conditions for 24 h. Quantification of biofilms was performed as described above. The percentage of inhibition of biofilm formation was determined using the following formula as described by Zmantar et al (2017) . Each experiment was performed in triplicate.…”

Section: Evaluation Of Anti-biofilm Efficacy Of Antimicrobial Agents ...mentioning

confidence: 99%

Antibacterial and antibiofilm effects of essential oil components, EDTA and HLE disinfectant solution on Enterococcus, Pseudomonas and Staphylococcus sp. multiresistant strains isolated along the meat production chain

Gómez

Manetsberger²,

Benomar³

et al. 2022

Front. Microbiol.

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The spread of multidrug resistant (MDR) bacteria and resistance genes along the food chain and the environment has become a global, but silent pandemic. To face this challenge, it is of outmost importance to develop efficient strategies to reduce potential contamination by these agents. In the present study, 30 strains of Enterococcus sp., Staphylococcus sp. and Pseudomonas sp. isolated from various surfaces throughout the meat production chain in a goat and lamb slaughterhouse were characterized as MDR bacteria harboring several antibiotic resistance genes (ARGs). The antimicrobial efficacy of natural essential oil components “EOCs” (carvacrol “CA,” cinnamaldehyde “CIN,” eugenol “EU,” geraniol “GE,” limonene “LI” and thymol “TH”), HLE disinfectant solution (3–6% H2O2; 2.2–4.4% lactic acid and 12.5–25 mM EDTA in water) and EDTA was tested against these MDR bacteria. Results showed that Minimum Inhibitory Concentrations (MIC) were compound and strain dependent. In addition, the synergistic effect of these antimicrobials was evaluated at 1/2 MIC. Here our study showed particularly promising results regarding the inhibitory effect at sub-inhibitory concentrations, which were confirmed by the analysis of bacterial growth dynamics over 72 h. Furthermore, the inhibitory effect of EOCs, HLE disinfectant solution and EDTA or their combinations was studied in developing and established biofilms of MDR bacteria obtaining variable results depending on the morphological structure of the tested strain and the phenolic character of the EOCs. Importantly, the combination of EOCs with HLE or EDTA showed particularly positive results given the effective inhibition of biofilm formation. Moreover, the synergistic combinations of EU and HLE/EDTA, TH, CA, GE, LI or CIN + EDTA/HLE caused log reductions in established biofilms of several strains (1–6 log10 CFU) depending on the species and the combination used, with Pseudomonas sp. strains being the most susceptible. Given these results, we propose novel antimicrobial formulations based on the combination of sub-inhibitory concentrations of EOCs and HLE or EDTA as a highly promising alternative to currently used approaches. This novel strategy notably shows great potential to efficiently decrease the emergence and spread of MDR bacteria and ARGs in the food chain and the environment, thus supporting the decrease of resistomes and pathogenesis in clinical and industrial areas while preserving the antibiotic therapeutic action.

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“…Then, 200 µl PBS were added to the wells, and the absorbance at 590 nm was determined using a microplate reader (iMark Microplate Absorbance Reader, Bio-Rad instrument). The percentage of inhibition of biofilm formation was determined using the following formula as described by Zmantar et al (2017):…”

Section: Determining the Effect Of Hle On Biofilm Developmentmentioning

confidence: 99%

Efficacy of “HLE”—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria

Abriouel

Lerma

Montoro

et al. 2018

Environmental Research

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We evaluated the efficacy of a new disinfectant product, HLE, to inhibit multiple species of planktonic and biofilm bacterial cultures. The HLE disinfectant comprised of EDTA, lactic acid and hydrogen peroxide, and our data indicated that the disinfectant had effective antimicrobial and anti-biofilm activity even at low concentrations (0.15% to 0.4% HLE, v/v). Furthermore, the HLE disinfectant destabilized biofilm structures eradicated them due to the synergistic effect of EDTA and both antimicrobials (lactic acid and hydrogen peroxide), as revealed by confocal laser scanning microscopy. Additionally, sub-inhibitory concentrations of HLE disinfectant, with EDTA as an efflux pump inhibitor, inhibited the expression of multidrug EfrAB, NorE and MexCD efflux pumps in both planktonic and biofilm cultures. This could provide an alternative way to disinfect surfaces to avoid spreading multi-drug resistant strains in the food chain and the environment by decreasing efflux pump expression and consequently reducing the antibiotic selective pressure caused by systemic antibiotics and disinfectant use.

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Modulation of drug resistance and biofilm formation of Staphylococcus aureus isolated from the oral cavity of Tunisian children

Abstract: LPE may be considered to have resistance-modifying activity. A more detailed investigation is necessary to determine the active compound responsible for therapeutic and disinfectant modulation.

Cited by 12 publications

References 30 publications

Antimicrobial Activity of Microgels with an Immobilized Copper(II) Complex Linked to Cross-Linking and Composition

Antimicrobial Activity of Microgels with an Immobilized Copper(II) Complex Linked to Cross-Linking and Composition

Antibacterial and antibiofilm effects of essential oil components, EDTA and HLE disinfectant solution on Enterococcus, Pseudomonas and Staphylococcus sp. multiresistant strains isolated along the meat production chain

Efficacy of “HLE”—a multidrug efflux-pump inhibitor—as a disinfectant against surface bacteria

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